Nutritional stimulation of commensal oral bacteria suppresses pathogens: The prebiotic concept
Open accessAbstract
AIM: To identify potential oral prebiotics that selectively stimulate commensal, albeit beneficial bacteria of the resident oral microbial community while suppressing the growth of pathogenic bacteria. MATERIAL AND METHODS: Using Phenotype MicroArrays as a high-throughput method, the change in respiratory activity of 16 oral bacteria in response to 742 nutritional compounds was screened. Most promising prebiotic compounds were selected and applied in single species growth and biofilm formation assays, as well as dual species (beneficial-pathogen) competition assays. RESULTS: Increased respiratory activity could not always be related to an increase in growth or biofilm formation. Six compounds were used in dual species competition assays to directly monitor if selective nutritional stimulation of the beneficial bacterium results in the suppression of the pathogenic bacterium. Two compounds, beta-methyl-d-galactoside and N-acetyl-d-mannosamine, could be identified as potential oral prebiotic compounds, triggering selectively beneficial oral bacteria throughout the experiments and shifting dual species biofilm communities towards a beneficial dominating composition at in vitro level. CONCLUSION: Our observations support the hypothesis that nutritional stimulation of beneficial bacteria by prebiotics could be used to restore the microbial balance in the oral cavity and by this promote oral health.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In 40 patients with generalized gingivitis, probiotic products containing bacilli significantly reduced plaque and gingivitis indices after 8 weeks, but there were no statistically significant differences between the placebo and probiotic groups.
“Plaque and gingivitis indices were significantly reduced after 8 weeks of treatment but showed no statistically significant differences between the placebo and probiotic groups [141].”
From A Cross-Talk between Diet and the Oral Microbiome: Balance of Nutrition on Inflammation and Immune System’s Response during Periodontitis (2022) · cites this paper as [141]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A Multiple Comparison Procedure for Comparing Several Treatments with a Control
Dunnett, C. W. (1955) · Journal of the American Statistical Association · doi:10.1080/01621459.1955.10501294 · Cited by 5,930 · see citing works
Dewhirst, F. E., Chen, T., Izard, J., Paster, B. J., Tanner, A. C. R., Yu, W.-H., Lakshmanan, A., & Wade, W. G. (2010) · Journal of Bacteriology · doi:10.1128/jb.00542-10 · Cited by 3,352 · see citing works
Defining the normal bacterial flora of the oral cavity
Aas, J. A., Paster, B. J., Stokes, L. N., Olsen, I., & Dewhirst, F. E. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.11.5721-5732.2005 · Cited by 3,092 · see citing works
Rectangular Confidence Regions for the Means of Multivariate Normal Distributions
Šidák, Z. (1967) · Journal of the American Statistical Association · doi:10.1080/01621459.1967.10482935 · Cited by 2,904 · see citing works
Dietary modulation of the human colonic microbiota: updating the concept of prebiotics
Gibson, G. R., Probert, H. M., Loo, J. V., Rastall, R. A., & Roberfroid, M. B. (2004) · Nutrition Research Reviews · doi:10.1079/nrr200479 · Cited by 2,539 · see citing works
The keystone-pathogen hypothesis
Hajishengallis, G., Darveau, R. P., & Curtis, M. A. (2012) · Nature Reviews Microbiology · doi:10.1038/nrmicro2873 · Cited by 1,790 · see citing works
Socransky, S. S., & Haffajee, A. D. (2005) · Periodontology 2000 · doi:10.1111/j.1600-0757.2005.00107.x · Cited by 1,691 · see citing works
Prebiotics: The Concept Revisited1,
Roberfroid, M. (2007) · Journal of Nutrition · doi:10.1093/jn/137.3.830s · Cited by 1,502 · see citing works
Rectangular Confidence Regions for the Means of Multivariate Normal Distributions
Šidák, Z. (1967) · Journal of the American Statistical Association · doi:10.2307/2283989 · Cited by 1,455 · see citing works
Microbial ecology of dental plaque and its significance in health and disease
Marsh, P. D. (1994) · Advances in Dental Research · doi:10.1177/08959374940080022001 · Cited by 1,278 · see citing works
Are dental diseases examples of ecological catastrophes?
Marsh, P. D. (2003) · Microbiology · doi:10.1099/mic.0.26082-0 · Cited by 1,256 · see citing works
Griffen, A. L., Beall, C. J., Campbell, J. H., Firestone, N. D., Kumar, P., Yang, Z. K., Podar, M., & Leys, E. J. (2011) · The ISME Journal · doi:10.1038/ismej.2011.191 · Cited by 1,006 · see citing works
Näse, L., Hatakka, K., Savilahti, E., Saxelin, M., Pönkä, A., Poussa, T. A., Korpela, R., & Meurman, J. H. (2001) · Caries Research · doi:10.1159/000047484 · Cited by 581 · see citing works
Teughels, W., Durukan, A., Özçelik, O., Pauwels, M., Quirynen, M., & Haytaç, M. C. (2013) · Journal Of Clinical Periodontology · doi:10.1111/jcpe.12155 · Cited by 329 · see citing works
Self-citationKumar, P., Leys, E. J., Bryk, J., Martinez, F. J., Moeschberger, M. L., & Griffen, A. L. (2006) · Journal of Clinical Microbiology · doi:10.1128/jcm.00317-06 · Cited by 318 · see citing works
Burton, J. P., Chilcott, C. N., Moore, C. J., Speiser, G., & Tagg, J. R. (2006) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2006.02837.x · Cited by 307 · see citing works
Probiotics Reduce the Prevalence of Oral Candida in the Elderly—a Randomized Controlled Trial
Hatakka, K., Ahola, A. J., Yli‐Knuuttila, H., Richardson, M. D., Poussa, T. A., Meurman, J. H., & Korpela, R. (2007) · Journal of Dental Research · doi:10.1177/154405910708600204 · Cited by 306 · see citing works
Microbial complexes in supragingival plaque
Haffajee, A. D., Socransky, S. S., Patel, M., & Song, X. (2008) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2007.00411.x · Cited by 279 · see citing works
How is the development of dental biofilms influenced by the host?
Marsh, P. D., & DeVine, D. (2011) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.2010.01673.x · Cited by 268 · see citing works
Controlling the oral biofilm with antimicrobials
Marsh, P. (2010) · Journal of Dentistry · doi:10.1016/s0300-5712(10)70005-1 · Cited by 253 · see citing works
Unstimulated salivary flow rate, pH and buffer capacity of saliva in healthy volunteers
Fenoll-Palomares, C., Muñoz-Montagud, J. V., Sánchiz, V., Herreros, B., Hernández, V., Mínguez, M., & Benages, A. (2004) · Revista Española de Enfermedades Digestivas · doi:10.4321/s1130-01082004001100005 · Cited by 238 · see citing works
STECKSÉN‐BLICKS, C., Sjöström, I., & Twetman, S. (2009) · Caries Research · doi:10.1159/000235581 · Cited by 186 · see citing works
Contemporary perspective on plaque control
Marsh, P. D. (2012) · BDJ · doi:10.1038/sj.bdj.2012.524 · Cited by 160 · see citing works
Kolenbrander, P. E., & Andersen, R. N. (1989) · Infection and Immunity · doi:10.1128/iai.57.10.3204-3209.1989 · Cited by 152 · see citing works
How do non-differentiating bacteria adapt to starvation?
Kjelleberg, S. J., Albertson, N. H., Fl�rdh, K., et al. (1993) · Antonie van Leeuwenhoek · doi:10.1007/bf00871228 · Cited by 149 · see citing works
Laleman, I., Yılmaz, E., Özçelik, O., Haytac, C. M., Pauwels, M., Herrero, E. R., Slomka, V., Quirynen, M., Alkaya, B., & Teughels, W. (2015) · Journal Of Clinical Periodontology · doi:10.1111/jcpe.12464 · Cited by 144 · see citing works
Self-citationTransport of galactose, glucose and their molecular analogues by Escherichia coli K12
Henderson, P. J. F., Giddens, R. A., & Jones-Mortimer, M. C. (1977) · Biochemical Journal · doi:10.1042/bj1620309 · Cited by 142 · see citing works
Takahashl, N., Saito, K., Schachtele, C. F., & YAMADA, T. (1997) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.1997.tb00733.x · Cited by 133 · see citing works
Antibacterial Activity of Red and White Wine against Oral Streptococci
Daglia, M., Papetti, A., Grisoli, P., Aceti, C., Dacarro, C., & Gazzani, G. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf070352q · Cited by 117 · see citing works
Socransky, S. S., Haffajee, A. D., Ximenez‐Fyvie, L., Feres, M., & Mager, D. L. (1999) · Periodontology 2000 · doi:10.1111/j.1600-0757.1999.tb00165.x · Cited by 113 · see citing works
Antimicrobial activity of Streptococcus salivarius K12 on bacteria involved in oral malodour
Masdea, L., Kulik, E. M., Hauser‐Gerspach, I., Ramseier, A. M., Filippi, A., & Waltimo, T. M. T. (2012) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2012.02.011 · Cited by 101 · see citing works
Preliminary assessment of safety and effectiveness in humans of ProBiora3 ™, a probiotic mouthwash
Zahradnik, R. T., Magnusson, I., Walker, C., McDonell, E., Hillman, C. H., & Hillman, J. D. (2009) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2009.04243.x · Cited by 93 · see citing works
Finney, M., Smullen, J., Foster, H. A., Brokx, S., & Storey, D. M. (2007) · European Journal of Nutrition · doi:10.1007/s00394-007-0666-7 · Cited by 88 · see citing works
Microbial Interactions Influence Inflammatory Host Cell Responses
Sliepen, I., Damme, J. V., Essche, M. V., Loozen, G., Quirynen, M., & Teughels, W. (2009) · Journal of Dental Research · doi:10.1177/0022034509347296 · Cited by 81 · see citing works
Self-citationLoozen, G., Boon, N., Pauwels, M., et al. (2011) · Molecular Oral Microbiology · doi:10.1111/j.2041-1014.2011.00615.x · Cited by 75 · see citing works
Self-citationNishihara, T., Suzuki, N., Yoneda, M., et al. (2014) · BMC Oral Health · doi:10.1186/1472-6831-14-110 · Cited by 72 · see citing works
Inter‐bacterial correlations in subgingival biofilms: a large‐scale survey
Loozen, G., Özçelik, O., Boon, N., Mol, A. D., Schoen, C., Quirynen, M., & Teughels, W. (2013) · Journal Of Clinical Periodontology · doi:10.1111/jcpe.12167 · Cited by 70 · see citing works
Self-citationDistribution of SalivaryLactobacillusandBifidobacteriumSpecies in Periodontal Health and Disease
Hojo, K., Mizoguchi, C., Taketomo, N., Ohshima, T., Gomi, K., Arai, T., & Maeda, N. (2007) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.60420 · Cited by 69 · see citing works
Takahashi, N., & Sato, T. (2002) · Oral Microbiology and Immunology · doi:10.1046/j.0902-0055.2001.00089.x · Cited by 61 · see citing works
Assimilation of lactitol, an 'unabsorbed' disaccharide in the normal human colon.
GRIMBLE, G. K., Patil, D. H., & Silk, D. B. A. (1988) · Gut · doi:10.1136/gut.29.12.1666 · Cited by 56 · see citing works
Dysbiosis by neutralizing commensal mediated inhibition of pathobionts
Herrero, E. R., Slomka, V., Boon, N., Bernaerts, K., Hernandez‐Sanabria, E., Quirynen, M., & Teughels, W. (2016) · Scientific Reports · doi:10.1038/srep38179 · Cited by 56 · see citing works
Self-citationUtilization of Sialic Acid by Viridans Streptococci
Byers, H. L., Homer, K. A., & Beighton, D. (1996) · Journal of Dental Research · doi:10.1177/00220345960750080701 · Cited by 53 · see citing works
Bacterial Antagonism Against Periodontopathogens
van Essche, M., Loozen, G., Godts, C., Boon, N., Pauwels, M., Quirynen, M., & Teughels, W. (2013) · Journal of Periodontology · doi:10.1902/jop.2012.120261 · Cited by 42 · see citing works
Self-citationJiang, Q., Stamatova, I., Kari, K., & Meurman, J. H. (2014) · Beneficial Microbes · doi:10.3920/bm2014.0054 · Cited by 41 · see citing works
Regulation of neuraminidase expression in Streptococcus pneumoniae
Gualdi, L., Hayre, J. K., Gerlini, A., et al. (2012) · BMC Microbiology · doi:10.1186/1471-2180-12-200 · Cited by 40 · see citing works
Anomeric specificity of D-xylose isomerase
Collyer, C. A., Goldberg, J. D., Viehmann, H., et al. (1992) · Biochemistry · doi:10.1021/bi00163a034 · Cited by 34 · see citing works
Studies of the Dental Properties of Lactitol Compared with Five Other Bulk Sweeteners in vitro
Grenby, T. H., Phillips, A., & Mistry, M. (2009) · Caries Research · doi:10.1159/000261199 · Cited by 32 · see citing works
Wilson, D. B. (1976) · Journal of Bacteriology · doi:10.1128/jb.126.3.1156-1165.1976 · Cited by 18 · see citing works
Peptide utilization by oral streptococci
Andersson, C., Sund, M.-L., & Linder, L. K. B. (1984) · Infection and Immunity · doi:10.1128/iai.43.2.555-560.1984 · Cited by 18 · see citing works
Zimmer, W., Wilson, M. J., Marsh, P. D., et al. (1991) · Microbial Ecology in Health and Disease · doi:10.3402/mehd.v4i5.7716 · Cited by 15 · see citing works
Galactose transporters discriminate steric anomers at the cell surface in yeast
Fukasawa, T., Sakurai, H., Nogi, Y., et al. (2009) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2009.00517.x · Cited by 13 · see citing works
The effect of toothbrushing on secretion rate, pH and buffering capacity of saliva
Ligtenberg, A., Brand, H. S., Bots, C. P., et al. (2006) · International Journal of Dental Hygiene · doi:10.1111/j.1601-5037.2006.00170.x · Cited by 13 · see citing works
N-Acetylmannosamine Digestion by Human Oral Bacteria
AMIR, S. M., Barker, S. A., & WOODBURY, S. A. (1965) · Nature · doi:10.1038/207979a0 · Cited by 10 · see citing works
Digestion of N-acetylmannosamine and N-acetylneuraminic Acid by Human Oral Bacteria
How, M. J., Long, V., & WOODBURY, S. A. (1967) · Nature · doi:10.1038/2141249a0 · Cited by 4 · see citing works
Flagged first, then by citations
Research works printed but not linked: 4
- Dewhirst, F. E., Chen, T., Izard, J., Paster, B. J., Tanner, A. C., Yu, W. H., Lakshmanan, A. & Wade, W. G. (2010) The human oral micro- biome. Journal of Bacteriology 192, 5002-5017.
- Roberfroid, M. (2007) Prebiotics: the concept revisited. Journal of Nutrition 137, 830s-837s.
- Van Essche, M., Loozen, G., Godts, C., Boon, N., Pauwels, M., Quirynen, M. & Teughels, W. (2013) Bacterial antagonism against periodon- topathogens. Journal of Periodontology 84, 801-811.
- Wilson, M. (2005) Role of the Indigenous Micro- biota in Maintaining Human Health Microbial Inhabitants of Humans. New York, NY: Cam- bridge University Press.
Cited by
Sorted by citations
Santonocito, S., Giudice, A., Polizzi, A., Troiano, G., Merlo, E. M., Sclafani, R., Grosso, G., & Isola, G. (2022) · Nutrients · doi:10.3390/nu14122426 · Cited by 144 · see citing works
Baker, J. L., & Edlund, A. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.03323 · Cited by 118 · see citing works
Oral prebiotics and the influence of environmental conditions in vitro
Slomka, V., Herrero, E. R., Boon, N., Bernaerts, K., Trivedi, H. M., Daep, C., Quirynen, M., & Teughels, W. (2018) · Journal of Periodontology · doi:10.1002/jper.17-0437 · Cited by 54 · see citing works
Self-citationChapter 13: Diet and periodontal diseases
Woelber, J. P., & Tennert, C. (2020) · Monographs in Oral Science · doi:10.1159/000455380 · Cited by 54 · see citing works
- Authors
- Slomka, V., Hernandez‐Sanabria, E., Herrero, E. R., Zaidel, L., Bernaerts, K., Boon, N., Quirynen, M., & Teughels, W.
- Affiliations
- KU Leuven (BE)
- Ghent University (BE)
- Colgate-Palmolive (United States) (US)
- Corresponding author
- Teughels, Wim
- ORCID
- Venue
- Journal Of Clinical Periodontology
- Publisher
- Wiley
- Published
- 2017-02-18
- Volume
- 44
- Issue
- 4
- Pages
- 344-352
- ISSN
- 0303-6979 (ISSN-L)
- 1600-051X
- OpenAlex
- W2580776317
- Cited by
- 67
- References
- 54
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 5 · 2023: 7 · 2022: 9 · 2021: 13 · 2020: 6 · 2019: 7 · 2018: 9 · 2017: 4
- FWCI
- 9.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal Of Clinical Periodontology (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Lirias (KU Leuven) (submittedVersion, other-oa)
- Funders
- Fonds Wetenschappelijk Onderzoek (G.0944.13)
- Colgate-Palmolive Company
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- oral microbiota, pathogen inhibition, biofilm formation, respiratory activity, N-acetylmannosamine, oral health, microbial balance
- MeSH terms
- Humans, Mouth, Oral Health, Symbiosis, Bacterial Physiological Phenomena, Prebiotics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
