Probiotic gut effect prevents the chronic psychological stress‐induced brain activity abnormality in mice
Abstract
Background A probiotic formulation (Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 combination, Probio'Stick(®) ) displays anxiolytic-like activity and reduces apoptosis in the lymbic system in animal models of depression. Based on the hypothesis that modulation of gut microbiota by this probiotic formulation has beneficial effects on brain activity in stress conditions, we report a set of probiotic-evoked physiological, cellular, and molecular events in the brain of Probio'Stick(®) pretreated mice submitted to chronic psychological stress. Methods Water avoidance stress (WAS) was applied or not (sham). Hypothalamic-pituitary-adrenal (HPA) axis and autonomic nervous system (ANS) responses to the chronic stress were assessed through plasma corticosterone and catecholamine measurements. Specific markers for neuronal activity, neurogenesis, and synaptic plasticity were used to assess brain activity. In addition, gut permeability and tight junction (TJ) proteins levels were also determinated. Key results We observed that a pretreatment with the probiotic formulation attenuated HPA axis and ANS activities in response to WAS, and reduced cFos expression in different brain areas but Lactobacillus salivarius (a negative control) treatment was ineffective on these parameters. Moreover, probiotic pretreatment prevented the WAS-induced decrease hippocampal neurogenesis and expression changes in hypothalamic genes involved in synaptic plasticity. These central effects we…
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Combination treatment of B. longum (R0175) and L. helveticus (R0052) increased hypothalamic BDNF and decreased GAP43, GFAP, Vim, Syt4, Snap25, and cell adhesion markers relative to a stressed control cohort.
“The combination treatment of B. longum (R0175) and L. helveticus (R0052) on neural plasticity genes, hypothalamic BDNF was increased whereas Growth Associated Protein 43 (GAP43), GFAP (glial fibrillary acid protein), Vim (vimentine), Syt4 (synaptotagmin), Snap25 (synaptic-associated protein 25 kDa) and cell adhesion markers (Reln, Sema [semaphorine]) were all decreased relative to a stressed”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Ait-Belgnaoui et al., 2014
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- Authors
- Ait‐Belgnaoui, A., Colom, A., Braniste, V., Ramalho, L., Marrot, A., Cartier, C., Houdeau, E., Theodorou, V., & Tompkins, T.
- Affiliations
- Neuro‐Gastroenterologie et Nutrition team, TOXALIM, UMR 1331‐INRA/INP/UPS Toulouse France
- Université Toulouse III - Paul Sabatier (FR)
- Lallemand (Canada) (CA)
- Venue
- Neurogastroenterology & Motility
- Publisher
- Wiley
- Published
- 2013-12-30
- Volume
- 26
- Issue
- 4
- Pages
- 510-520
- ISSN
- 1350-1925 (ISSN-L)
- 1365-2982
- OpenAlex
- W2087418736
- Cited by
- 434
- References
- 7
- Citations by year
- 2026: 18 · 2025: 34 · 2024: 43 · 2023: 40 · 2022: 57 · 2021: 47 · 2020: 42 · 2019: 33 · 2018: 41 · 2017: 29 · 2016: 22 · 2015: 18 · 2014: 10
- FWCI
- 24.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neurogastroenterology & Motility (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- probiotics, chronic psychological stress, water avoidance stress, Lactobacillus helveticus R0052, gut microbiota, hypothalamic-pituitary-adrenal axis, autonomic nervous system, corticosterone, catecholamines, hippocampal neurogenesis, synaptic plasticity, c-fos, hypothalamus, tight junctions, intestinal permeability, gut-brain axis
- MeSH terms
- Animals, Bifidobacterium, Brain, Chronic Disease, Hypothalamo-Hypophyseal System, Male, Mice, Inbred C57BL, Neuronal Plasticity, Pituitary-Adrenal System, Stress, Psychological, Probiotics, Gastrointestinal Tract, Mice, Lactobacillus helveticus, Neurogenesis
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