Cited by 49Neuroscience and Neuropharmacology ResearchCellular transport and secretionGenetics and Neurodevelopmental Disorders

AMPA-receptor activation regulates the diffusion of a membrane marker in parallel with dendritic spine motility in the mouse hippocampus

Richards, D. A., De Paola, V., Caroni, P., Gähwiler, B. H., McKinney, R. A.·2004·The Journal of Physiology

Abstract

Dendritic spines are the site of most excitatory connections in the hippocampus. We have investigated the diffusibility of a membrane-bound green fluorescent protein (mGFP) within the inner leaflet of the plasma membrane using Fluorescence Recovery After Photobleaching. In dendritic spines the diffusion of mGFP was significantly retarded relative to the dendritic shaft. In parallel, we have assessed the motility of dendritic spines, and found an inverse correlation between spine motility and the rate of diffusion of mGFP. We then tested the influence of glutamate receptor activation or blockade, and the involvement of the actin cytoskeleton (using latrunculin A) on spine motility and mGFP diffusion. These results show that glutamate receptors regulate the mobility of molecules in the inner leaflet of the plasma membrane through an action upon the actin cytoskeleton, suggesting a novel mechanism for the regulation of postsynaptic receptor density and composition.

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Registry

Crossref · 10.1113/jphysiol.2004.062091

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38 of 38 references verified0 citation errors · 0 not checkable

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  • Live-cell imaging techniques like FRAP and single molecule tracking changed the view that glutamate receptors are restricted to synapses.

    “Whereas reports based on static EM suggested that glutamate receptors are restricted to synapses (Nusser, 2000), live-cell imaging techniques like FRAP and single molecule tracking changed this view radically (Richards et al., 2004; Triller and Choquet, 2005).”

    From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Richards et al., 2004

  • Mushroom-shaped spines form a membrane compartment in which diffusion is slowed.

    “indicating that mushroom-shaped spines form a membrane compartment in which diffusion is slowed.”

    From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Richards et al., 2004

  • Fluorescently-tagged plasma membrane probes in spines showed a faster recovery rate in stubby than in mushroom-shaped spines.

    “Furthermore, fluorescently-tagged plasma membrane probes in spines of different morphologies showed a faster recovery rate in stubby than in mushroom-shaped spines (Richards et al., 2004; Ashby et al., 2006), indicating that mushroom-shaped spines form a membrane compartment in which diffusion is slowed.”

    From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Richards et al., 2004

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Authors
Richards, D. A., De Paola, V., Caroni, P., Gähwiler, B. H., & McKinney, R. A.
Affiliations
  • University of Wisconsin–Madison (US)
  • Friedrich Miescher Institute (CH)
  • University of Zurich (CH)
Corresponding author
Richards, D. A.
Venue
The Journal of Physiology
Publisher
Wiley
Published
2004-07
Volume
558
Issue
2
Pages
503-512
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
OpenAlex
W2117914181
Cited by
49
References
39
Citations by year
2023: 1 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 2
FWCI
2.58
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
Keywords
AMPA receptors, membrane diffusion, hippocampus, glutamate receptor activation, actin cytoskeleton, latrunculin A
MeSH terms
Actins, Animals, Cell Membrane, Diffusion, Hippocampus, Mice, Transgenic, Organ Culture Techniques, Receptors, AMPA, Fluorescence Recovery After Photobleaching, Dendritic Spines, Green Fluorescent Proteins, Mice

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