Specialized DNA Polymerases, Cellular Survival, and the Genesis of Mutations
Abstract
Cell death caused by arrested replication of damaged or structurally altered DNA can be avoided in prokaryotic and eukaryotic cells by multiple DNA polymerases that are specialized to bypass DNA damage. Some of these polymerases perform such translesion DNA synthesis of specific types of damage with high genetic fidelity. However, they exhibit greatly reduced fidelity when they operate on undamaged DNA or on DNA with lesions that are (apparently) not cognate substrates. The low fidelity of some of these specialized polymerases when copying undamaged DNA may be physiologically functional, including generating immunoglobulin diversity.
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Translesion synthesis (TLS) polymerases copy past lesions in DNA that block the major replicative polymerases.
“Translesion synthesis (TLS) polymerases copy past lesions in DNA that block the major replicative polymerases (7)(8)(9)(10)(11).”
From DNA Replication Fidelity (2004) · cites this paper as [9]
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- Authors
- Friedberg, E. C., Wagner, R., & Radman, M.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Gene Check (United States) (US)
- Inserm (FR)
- Corresponding author
- Friedberg, Errol C.
- ORCID
- Radman, Miroslav
- Venue
- Science
- Published
- 2002-05-31
- Volume
- 296
- Issue
- 5573
- Pages
- 1627-1630
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2102334286
- Cited by
- 443
- References
- 54
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 3 · 2023: 9 · 2022: 3 · 2021: 5 · 2020: 9 · 2019: 8 · 2018: 13 · 2017: 13 · 2016: 11 · 2015: 15 · 2014: 22 · 2013: 9 · 2012: 17
- FWCI
- 4.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- translesion synthesis, DNA damage bypass, replication fidelity, mutagenesis, cell survival, immunoglobulin diversity, V(D)J recombination, prokaryotic and eukaryotic cells
- MeSH terms
- Animals, Bacteria, Cell Survival, DNA, DNA Damage, DNA-Directed DNA Polymerase, DNA Replication, Genes, Immunoglobulin, Humans, Templates, Genetic, Yeasts, Mutagenesis, Evolution, Molecular, Somatic Hypermutation, Immunoglobulin
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