Cited by 705Cellular transport and secretionAutophagy in Disease and TherapyLipid metabolism and biosynthesis

Role of LBPA and Alix in Multivesicular Liposome Formation and Endosome Organization

Open access
Matsuo, Hirotami, Chevallier, Julien, Mayran, Nathalie, Le Blanc, Isabelle, Ferguson, Charles, Fauré, Julien, Blanc, Nathalie Sartori, Matile, Stefan +5 more·2004·Science

Abstract

What are the components that control the assembly of subcellular organelles in eukaryotic cells? Although membranes can clearly be distorted by cytosolic factors, very little is known about the intrinsic mechanisms that control the biogenesis, shape, and organization of organellar membranes. Here, we found that the unconventional phospholipid lysobisphosphatidic acid (LBPA) could induce the formation of multivesicular liposomes that resembled the multivesicular endosomes that exist where this lipid is found in vivo. This process depended on the same pH gradient that exists across endosome membranes in vivo and was selectively controlled by Alix. In turn, Alix regulated the organization of LBPA-containing endosomes in vivo.

Integrity signals

Registry

Crossref · 10.1126/science.1092425

Cites

26 of 26 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • LBPA is functionally involved in membrane degradation by recruiting PDCD6IP.

    “LBPA is also functionally involved in membrane degradation by recruiting the programmed cell death-6-interacting protein (PDCD6IP) (45, 46).”

    From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [45]

  • Inward budding of the endosomal limiting membrane is driven by BMP, a cone-shaped lipid that induces spontaneous negative curvature, resulting in endovesiculation and ILV formation.

    “This inward budding is driven by a unique negatively-charged lipid called BMP that accumulates in late endosomal membranes. Like ceramide, BMP is a cone-shaped lipid that induces spontaneous negative curvature in membranes, resulting in endovesiculation and ILV formation (Matsuo et al., 2004).”

    From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Matsuo et al., 2004

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Matsuo, H., Chevallier, J., Mayran, N., Le Blanc, I., Ferguson, C., Fauré, J., Blanc, N. S., Matile, S., Dubochet, J., Sadoul, R., Parton, R. G., Vilbois, F., & Gruenberg, J.
Affiliations
  • University of Geneva (CH)
  • Department of Biochemistry, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva 4, Switzerland.
Corresponding author
  • Matsuo, Hirotami
  • Gruenberg, Jean
Venue
Science
Published
2004-01-23
Volume
303
Issue
5657
Pages
531-534
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2135859072
Cited by
705
References
26
Citations by year
2026: 18 · 2025: 20 · 2024: 30 · 2023: 24 · 2022: 31 · 2021: 37 · 2020: 33 · 2019: 24 · 2018: 34 · 2017: 22 · 2016: 25 · 2015: 28 · 2014: 35 · 2013: 38 · 2012: 32
FWCI
18.17
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • IRIS (submittedVersion)
  • SERVAL (Université de Lausanne) (submittedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
lysobisphosphatidic acid, Alix, multivesicular endosomes, pH gradient, membrane biogenesis
MeSH terms
Animals, Arylsulfonates, Calcium-Binding Proteins, Carrier Proteins, Cell Line, Cytosol, Coloring Agents, Endocytosis, HeLa Cells, Humans, Hydrogen-Ion Concentration, Lipid Bilayers, Liposomes, Lysophospholipids, Membrane Glycoproteins, Endosomes, Vesicular stomatitis Indiana virus, Viral Envelope Proteins, Molecular Structure, Annexin A2, Cell Cycle Proteins, RNA Interference, RNA, Small Interfering, Monoglycerides, Endosomal Sorting Complexes Required for Transport

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review