Role of LBPA and Alix in Multivesicular Liposome Formation and Endosome Organization
Open accessAbstract
What are the components that control the assembly of subcellular organelles in eukaryotic cells? Although membranes can clearly be distorted by cytosolic factors, very little is known about the intrinsic mechanisms that control the biogenesis, shape, and organization of organellar membranes. Here, we found that the unconventional phospholipid lysobisphosphatidic acid (LBPA) could induce the formation of multivesicular liposomes that resembled the multivesicular endosomes that exist where this lipid is found in vivo. This process depended on the same pH gradient that exists across endosome membranes in vivo and was selectively controlled by Alix. In turn, Alix regulated the organization of LBPA-containing endosomes in vivo.
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LBPA is functionally involved in membrane degradation by recruiting PDCD6IP.
“LBPA is also functionally involved in membrane degradation by recruiting the programmed cell death-6-interacting protein (PDCD6IP) (45, 46).”
From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [45]
Inward budding of the endosomal limiting membrane is driven by BMP, a cone-shaped lipid that induces spontaneous negative curvature, resulting in endovesiculation and ILV formation.
“This inward budding is driven by a unique negatively-charged lipid called BMP that accumulates in late endosomal membranes. Like ceramide, BMP is a cone-shaped lipid that induces spontaneous negative curvature in membranes, resulting in endovesiculation and ILV formation (Matsuo et al., 2004).”
From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Matsuo et al., 2004
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- Authors
- Matsuo, H., Chevallier, J., Mayran, N., Le Blanc, I., Ferguson, C., Fauré, J., Blanc, N. S., Matile, S., Dubochet, J., Sadoul, R., Parton, R. G., Vilbois, F., & Gruenberg, J.
- Affiliations
- University of Geneva (CH)
- Department of Biochemistry, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva 4, Switzerland.
- Corresponding author
- Matsuo, Hirotami
- Gruenberg, Jean
- Venue
- Science
- Published
- 2004-01-23
- Volume
- 303
- Issue
- 5657
- Pages
- 531-534
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2135859072
- Cited by
- 705
- References
- 26
- Citations by year
- 2026: 18 · 2025: 20 · 2024: 30 · 2023: 24 · 2022: 31 · 2021: 37 · 2020: 33 · 2019: 24 · 2018: 34 · 2017: 22 · 2016: 25 · 2015: 28 · 2014: 35 · 2013: 38 · 2012: 32
- FWCI
- 18.17
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- lysobisphosphatidic acid, Alix, multivesicular endosomes, pH gradient, membrane biogenesis
- MeSH terms
- Animals, Arylsulfonates, Calcium-Binding Proteins, Carrier Proteins, Cell Line, Cytosol, Coloring Agents, Endocytosis, HeLa Cells, Humans, Hydrogen-Ion Concentration, Lipid Bilayers, Liposomes, Lysophospholipids, Membrane Glycoproteins, Endosomes, Vesicular stomatitis Indiana virus, Viral Envelope Proteins, Molecular Structure, Annexin A2, Cell Cycle Proteins, RNA Interference, RNA, Small Interfering, Monoglycerides, Endosomal Sorting Complexes Required for Transport
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