Cited by 267Neuroscience and Neuropharmacology ResearchIon channel regulation and functionPI3K/AKT/mTOR signaling in cancer

Activity- and mTOR-dependent suppression of Kv1.1 channel mRNA translation in dendrites

Raab-Graham, Kimberly F., Haddick, Patrick C. G., Jan, Yuh Nung, Jan, Lily Yeh·2006·Science

Abstract

Mammalian target of rapamycin (mTOR) is implicated in synaptic plasticity and local translation in dendrites. We found that the mTOR inhibitor, rapamycin, increased the Kv1.1 voltage-gated potassium channel protein in hippocampal neurons and promoted Kv1.1 surface expression on dendrites without altering its axonal expression. Moreover, endogenous Kv1.1 mRNA was detected in dendrites. Using Kv1.1 fused to the photoconvertible fluorescence protein Kaede as a reporter for local synthesis, we observed Kv1.1 synthesis in dendrites upon inhibition of mTOR or the N-methyl-d-aspartate (NMDA) glutamate receptor. Thus, synaptic excitation may cause local suppression of dendritic Kv1 channels by reducing their local synthesis.

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Crossref · 10.1126/science.1131693

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28 of 28 references verified0 citation errors · 0 not checkable

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Authors
Raab-Graham, K. F., Haddick, P. C. G., Jan, Y. N., & Jan, L. Y.
Affiliations
  • Howard Hughes Medical Institute, Departments of Physiology and Biochemistry, University of California, San Francisco, CA 94158, USA.
  • Howard Hughes Medical Institute (US)
Corresponding author
  • Haddick, Patrick C. G.
  • Jan, Lily Yeh
Venue
Science
Published
2006-10-06
Volume
314
Issue
5796
Pages
144-148
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2028230396
Cited by
267
References
28
Citations by year
2026: 3 · 2025: 5 · 2024: 7 · 2023: 10 · 2022: 7 · 2021: 5 · 2020: 23 · 2019: 13 · 2018: 9 · 2017: 15 · 2016: 15 · 2015: 18 · 2014: 17 · 2013: 25 · 2012: 21
FWCI
5.06
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (1f32mh013010-01)
  • National Institute of Mental Health (MH13010, MH65334)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
Keywords
Kv1.1, voltage-gated potassium channels, mTOR, local translation, dendrites, hippocampal neurons, rapamycin, NMDA receptor, synaptic plasticity, mRNA translation, channel surface expression, synaptic excitation
MeSH terms
Animals, Cells, Cultured, Dendrites, Hippocampus, Neuronal Plasticity, Neurons, Phosphorylation, Protein Kinases, Recombinant Fusion Proteins, RNA, Messenger, Sindbis Virus, Synapses, Protein Biosynthesis, Receptors, N-Methyl-D-Aspartate, Rats, Sprague-Dawley, Excitatory Postsynaptic Potentials, Sirolimus, Oligonucleotide Array Sequence Analysis, 3' Untranslated Regions, Protein Kinase Inhibitors, Rats, Kv1.1 Potassium Channel, TOR Serine-Threonine Kinases, In Vitro Techniques

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