Cited by 4,173RNA Interference and Gene DeliveryVirus-based gene therapy researchIntramuscular injections and effects

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Direct Gene Transfer into Mouse Muscle in Vivo

Wolff, Jon A., Malone, Robert W., Williams, Phillip, Chong, Wang, Acsadi, Gyula, Jani, Agnes, Felgner, Philip L.·1990·Science

DOI 10.1126/science.1690918

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Abstract

RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and β-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for β-galactosidase activity was localized to muscle cells following injection of the β-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.

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Authors: Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L.
Affiliations: University of Wisconsin–Madison (US)
Vical (United States) (US)
Departments of Pediatrics and Genetics, Waisman Center, University of Wisconsin, Madison, WI 53706.
ORCID: Malone, Robert W.
Felgner, Philip L.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1990-03-23
Volume: 247
Issue: 4949
Pages: 1465-1468
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1690918
OpenAlex: W2014495927
Cited by: 4,173
References: 27
Citations by year: 2026: 96 · 2025: 151 · 2024: 186 · 2023: 201 · 2022: 188 · 2021: 187 · 2020: 81 · 2019: 74 · 2018: 62 · 2017: 66 · 2016: 56 · 2015: 77 · 2014: 68 · 2013: 93 · 2012: 90
FWCI: 26.43
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5k11hd000669-02)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 03352, HD00669-05, P30HD003352)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Health Sciences › Medicine › Emergency Medicine › Intramuscular injections and effects
Keywords: in vivo gene delivery, skeletal muscle, intramuscular injection, DNA vectors, chloramphenicol acetyltransferase, luciferase, beta-galactosidase, transgene expression, cytochemical staining, transfection
MeSH terms: Animals, Avian Sarcoma Viruses, Coleoptera, beta-Galactosidase, DNA, Escherichia coli, Genetic Vectors, Histocytochemistry, Luciferases, Muscles, RNA, Transfection, Chloramphenicol O-Acetyltransferase, Gene Expression, Mice

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Direct Gene Transfer into Mouse Muscle in Vivo

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