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HIV-1 Entry Cofactor: Functional cDNA Cloning of a Seven-Transmembrane, G Protein-Coupled Receptor
Open accessAbstract
A cofactor for HIV-1 (human immunodeficiency virus-type 1) fusion and entry was identified with the use of a novel functional complementary DNA (cDNA) cloning strategy. This protein, designated “fusin,” is a putative G protein-coupled receptor with seven transmembrane segments. Recombinant fusin enabled CD4-expressing nonhuman cell types to support HIV-1 Env-mediated cell fusion and HIV-1 infection. Antibodies to fusin blocked cell fusion and infection with normal CD4-positive human target cells. Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line-tropic isolates, in comparison to its activity with macrophage-tropic HIV-1 isolates.
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Metadata
- Authors
- Feng, Y., Broder, C. C., Kennedy, P. E., & Berger, E. A.
- Venue
- Science
- Published
- 1996-05-10
- Volume
- 272
- Issue
- 5263
- Pages
- 872-877
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W82820957
- Cited by
- 4,172
- References
- 0
- FWCI
- 110.35
- Type
- journal article
- Language
- en
- Open access
- green
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