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A Role for a 70-Kilodalton Heat Shock Protein in Lysosomal Degradation of Intracellular Proteins
Abstract
A 73-kilodalton (kD) intracellular protein was found to bind to peptide regions that target intracellular proteins for lysosomal degradation in response to serum withdrawal. This protein cross-reacted with a monoclonal antibody raised to a member of the 70-kD heat shock protein (hsp70) family, and sequences of two internal peptides of the 73-kD protein confirm that it is a member of this family. In response to serum withdrawal, the intracellular concentration of the 73-kD protein increased severalfold. In the presence of adenosine 5′-triphosphate (ATP) and MgCl 2 , the 73-kD protein enhanced protein degradation in two different cell-free assays for lysosomal proteolysis.
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(2012) · PubMed
Metadata
- Authors
- Chiang, H.-L., Terlecky, S. R., Plant, C. P., & Dice, J. F.
- Venue
- Science
- Published
- 1989-10-20
- Volume
- 246
- Issue
- 4928
- Pages
- 382-385
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2013944211
- Cited by
- 981
- References
- 0
- FWCI
- 11.78
- Type
- journal article
- Language
- en
- Open access
- closed
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