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Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Open accessAbstract
CRISPR Cas9 molecular scissors: The CRISPR-associated (Cas) protein Cas9 is a molecular scissor for cutting DNA. The first step in the cutting reaction is the RNA-guided unwinding of the DNA double helix. Jiang et al. determined the structures of Cas9 bound to DNA unwound by the targeting RNA (see the Perspective by Chen and Bailey). Cas9 bends the DNA to allow guide RNA infiltration into the double helix. The two separated DNA strands, one bound to RNA, are subsequently positioned in the dual active sites of the protein for cutting. Science , this issue p. 867 ; see also p. 811
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Metadata
- Authors
- Jiang, F., Taylor, D. W., Chen, J. S., Kornfeld, J. E., Zhou, K., Thompson, A. J., Nogales, E., & Doudna, J. A.
- Venue
- Science
- Published
- 2016-02-19
- Volume
- 351
- Issue
- 6275
- Pages
- 867-871
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2237671266
- Cited by
- 624
- References
- 11
- FWCI
- 33.41
- Type
- journal article
- Language
- en
- Open access
- green
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