Cited by 182Fungal and yeast genetics researchAntifungal resistance and susceptibilityFungal Infections and Studies

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Expression, cloning, and characterization of a Candida albicans gene, ALA1, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins

Open access

Gaur, N K, Klotz, S A·1997·Infection and Immunity

DOI 10.1128/iai.65.12.5289-5294.1997PMC PMC175761

Open this paper in CoChat — free account

Abstract

Adherence of Candida albicans to host tissues is a necessary step for maintenance of its commensal status and is likely a necessary step in the pathogenesis of candidiasis. The extracellular matrix (ECM) proteins are some of the host tissue and plasma proteins to which C. albicans adheres through adhesins located on the fungal cell surface. To isolate genes encoding ECM adhesins, an assay was developed based on the ability of yeast cells to adhere to magnetic beads coated with the ECM protein fibronectin, type IV collagen, or laminin. A C. albicans genomic library was constructed by cloning XbaI-partially-digested and size-selected fragments into pAUR112, an Escherichia coli-yeast low-copy-number shuttle vector. The C. albicans library was transformed into Saccharomyces cerevisiae YPH 499, and clones capable of adherence were selected by using ECM protein-coated magnetic beads. A plasmid containing an approximately 8-kb insert was isolated from 29 adherent clones. These clones exhibited adherence to all ECM protein-coated magnetic beads and to human buccal epithelial cells. The ALA1 gene (for agglutinin-like adhesin) was localized by subcloning it into a 5-kb XbaI fragment which retained the adherence phenotype in both orientations. The complete DNA sequence of the 5-kb insert was determined, and an open reading frame (ORF) encoding 1,419 amino acid residues was identified. Deletions from the 5' and 3' ends extending into the DNA sequence encoding the 1,419-amino-acid ORF product inactivated the adherence phenotype, suggesting that it is the coding region of the ALA1 gene. A database search identified ALA1 to be similar to the C. albicans ALS1 (for agglutinin-like sequence 1) protein and the S. cerevisiae agglutinin protein (AG alpha1), although the homology at the primary amino acid sequence level is limited to the first half of each of these proteins. ALA1 contains a central domain of six tandem repeats of 36 amino acids. We discuss the significance of various predicted ALA1 structural motifs and their relationships to function in the adherence process.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1128/iai.65.12.5289-5294.1997

Cites

0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

MSCRAMM-MEDIATED ADHERENCE OF MICROORGANISMS TO HOST TISSUES
Patti, J. M., Allen, B. L., McGavin, M. J., & Höök, M. (1994) · Annual Review of Microbiology · doi:10.1146/annurev.mi.48.100194.003101 · Cited by 1,191
Adherence and receptor relationships of Candida albicans
Calderone, R. A., & Braun, P. C. (1991) · Microbiological Reviews · doi:10.1128/mr.55.1.1-20.1991 · Cited by 427

Show all 0 references

Research works printed but not linked: 3

[2]2. Ballou, C. E. 1988. Organization of the Saccharomyces cerevisiae cell wall, p. 105-117. In J. E. Varner (ed.), Self-assembling architecture. Alan R. Liss, New York, N.Y.
[10]10. Gale, C., D. Finkel, N. Tao, M. Meinke, M. McClellan, J. Olson, K. Kendrick, and M. Hostetter. 1996. Cloning and expression of a gene encoding an integrin-like protein in Candida albicans . Proc. Natl. Acad. Sci. USA 93: 357361.
[19]19. Lipke, P. N. 1996. Cell adhesion proteins in the nonvertebrate eukaryotes, p. 119-157. In W. E. G. Muller and G. Csaba (ed.), Cell surface associated receptors and their ligands in invertebrates and protists. Springer-Verlag, New York, N.Y.

Sorted by citations

Virulence factors of Candida albicans
Calderone, R. A., & Fonzi, W. A. (2001) · Trends in Microbiology · doi:10.1016/s0966-842x(01)02094-7 · Cited by 1,331
Cell Wall and Secreted Proteins of Candida albicans: Identification, Function, and Expression
Chaffin, W. L., López-Ribot, J. L., Casanova, M., Gozalbo, D., & Martínez, J. P. (1998) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.62.1.130-180.1998 · Cited by 726
Als3 Is a Candida albicans Invasin That Binds to Cadherins and Induces Endocytosis by Host Cells
Phan, Q. T., Myers, C. L., Fu, Y., Sheppard, D. C., Yeaman, M. R., Welch, W. H., Ibrahim, A. S., Edwards, J. E., & Filler, S. G. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050064 · Cited by 641
Candida albicans: A molecular revolution built on lessons from budding yeast
Berman, J., & Sudbery, P. E. (2002) · Nature Reviews Genetics · doi:10.1038/nrg948 · Cited by 578
The ALS gene family of Candida albicans.
Hoyer, L. L. (2001) · Trends in Microbiology · doi:10.1016/s0966-842x(01)01984-9 · Cited by 540
An Adhesin of the Yeast Pathogen Candida glabrata Mediating Adherence to Human Epithelial Cells
Cormack, B. P., Ghori, N., & Falkow, S. (1999) · Science · doi:10.1126/science.285.5427.578 · Cited by 383
Oral colonization by Candida albicans.
Cannon, R. D., & Chaffin, W. (1999) · Critical Reviews in Oral Biology and Medicine · doi:10.1177/10454411990100030701 · Cited by 357
Discovering the secrets of the Candida albicans agglutinin-like sequence (ALS) gene family – a sticky pursuit
Hoyer, L. L., Green, C. B., Oh, S.-H., & Zhao, X. (2008) · Medical Mycology · doi:10.1080/13693780701435317 · Cited by 356
Candida albicans Als3, a multifunctional adhesin and invasin
Liu, Y., & Filler, S. G. (2011) · Eukaryotic Cell · doi:10.1128/ec.00279-10 · Cited by 354
“At the N terminus is a signal peptide followed by a 300-amino-acid immunoglobulin-like domain and a 104-amino-acid threonine-rich domain that contains -sheets (18, 22, 47, 55).”
Background
A Human-Curated Annotation of the Candida albicans Genome
Braun, B. R., van het Hoog, M., d'Enfert, C., Martchenko, M., Dungan, J., Kuo, A., Inglis, D. O., Uhl, M. A., Hogues, H., Berriman, M., et al. (2005) · PLoS Genetics · doi:10.1371/journal.pgen.0010001 · Cited by 342
“albicans genome project [38,52–54]) and were aided greatly by information that emerged as the genome sequencing effort progressed [55–57].”
Background
The cell wall architecture of Candida albicans wild‐type cells and cell wall‐defective mutants
Kapteyn, J. C., Hoyer, L. L., Hecht, J. E., Müller, W. H., Andel, A., Verkleij, A. J., Makarow, M., Van Den Ende, H., & Klis, F. M. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.01729.x · Cited by 328
“cerevisiae confers adherence to human cells (Gaur and Klotz, 1997; Fu et al., 1998), suggesting that ALS genes may encode adhesins.”
Background
Adhesins in human fungal pathogens: glue with plenty of stick.
de Groot, P. W. J., Bader, O., de Boer, A. D., Weig, M., & Chauhan, N. (2013) · Eukaryotic Cell · doi:10.1128/ec.00364-12 · Cited by 319
Human pathogens utilize host extracellular matrix proteins laminin and collagen for adhesion and invasion of the host
Singh, B., Fleury, C., Jalalvand, F., & Riesbeck, K. (2012) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2012.00340.x · Cited by 302
Functional and Structural Diversity in the Als Protein Family of Candida albicans
Sheppard, D. C., Yeaman, M. R., Welch, W. H., Phan, Q. T., Fu, Y., Ibrahim, A. S., Filler, S. G., Zhang, M., Waring, A. J., & Edwards, J. E. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m401929200 · Cited by 300
A Biochemical Guide to Yeast Adhesins: Glycoproteins for Social and Antisocial Occasions
Dranginis, A. M., Rauceo, J. M., Coronado, J. E., & Lipke, P. N. (2007) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00037-06 · Cited by 294
ALS3 and ALS8 represent a single locus that encodes a Candida albicans adhesin; functional comparisons between Als3p and Als1p
Zhao, X., Oh, S.-H., Cheng, G., Green, C. B., Nuessen, J. A., Yeater, K., Leng, R. P., Brown, A. J. P., & Hoyer, L. L. (2004) · Microbiology · doi:10.1099/mic.0.26943-0 · Cited by 254
“The emergence of initial functional data for Als1p and Als5p is important because they have the most similar N-terminal domain sequences (87% amino acid identity) within the ALS family (Hoyer et al., 1995; Gaur & Klotz, 1997; Hoyer & Hecht, 2000; Fu et al., 2002).”
Influential
Candida albicans binds human plasminogen: identification of eight plasminogen‐binding proteins
Crowe, J. D., Sievwright, I. K., Auld, G. C., Moore, N. R., Gow, N. A. R., & Booth, N. A. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03390.x · Cited by 246
“, 1995), collagen, fibronectin and laminin (Gaur and Klotz, 1997).”
Background
Candida albicans ALS3 and insights into the nature of the ALS gene family
Hoyer, L. L., Payne, T. L., Bell, M., Myers, A. M., & Scherer, S. (1998) · Current Genetics · doi:10.1007/s002940050359 · Cited by 242
“recently demonstrated lending support to this hypothesis (Gaur and Klotz 1997).”
ResultInfluential
The Cell Wall-Associated Glyceraldehyde-3-Phosphate Dehydrogenase of Candida albicans Is Also a Fibronectin and Laminin Binding Protein
Gozalbo, D., Gil-Navarro, I., Azorín, I., Renau-Piqueras, J., Martínez, J. P., & Gil, M. (1998) · Infection and Immunity · doi:10.1128/iai.66.5.2052-2059.1998 · Cited by 231
“(4) and for the ALA1 gene product (15).”
Background
Interaction of Candida albicans with host cells: virulence factors, host defense, escape strategies, and the microbiota
Hoefs, S., Mogavero, S., & Hube, B. (2016) · The Journal of Microbiology · doi:10.1007/s12275-016-5514-0 · Cited by 251
“The eight members that are encoded by the ALS gene family (ALS1-7 and ALS9) are cell surface proteins and are glycosylphosphatidylinositol (GPI)linked to the ß-1,6-glucans of the fungal cell wall (Gaur and Klotz, 1997; Hoyer, 2001; Sheppard et al., 2004).”
BackgroundInfluential
Interactions of Candida albicans with epithelial cells
Zhu, W., & Filler, S. G. (2010) · Cellular Microbiology · doi:10.1111/j.1462-5822.2009.01412.x · Cited by 223
“encodes eight glycosylphosphatidylinositol (GPI)-linked cell surface proteins that mediate binding to diverse host substrates (Gaur and Klotz, 1997; Hoyer, 2001; Sheppard et al., 2004; Zhao et al., 2004; 2005; Braun et al., 2005).”
BackgroundInfluential
Antifungal proteins: targets, mechanisms and prospective applications
Theis, T., & Stahl, U. (2004) · Cellular and Molecular Life Sciences (CMLS) · doi:10.1007/s00018-003-3231-4 · Cited by 222
Usefulness of Multilocus Sequence Typing for Characterization of Clinical Isolates of Candida albicans
Bougnoux, M., Morand, S., & d'Enfert, C. (2002) · Journal of Clinical Microbiology · doi:10.1128/jcm.40.4.1290-1297.2002 · Cited by 216
“c CaSYA1 is the ortholog of the ScALA1 gene encoding alanyl-tRNA synthetase; however, the designation CaALA1 could not be used because it corresponds to a gene encoding an agglutinin-like adhesin (11).”
Background
Origins of variation in the fungal cell surface
Verstrepen, K. J., Reynolds, T. B., & Fink, G. R. (2004) · Nature Reviews Microbiology · doi:10.1038/nrmicro927 · Cited by 204
Binding of Candida albicans enolase to plasmin(ogen) results in enhanced invasion of human brain microvascular endothelial cells
Jong, A. Y., Chen, S. H. M., Stins, M. F., Kim, K. S., Tuan, T.-L., & Huang, S.-H. (2003) · Journal of Medical Microbiology · doi:10.1099/jmm.0.05060-0 · Cited by 206
“Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Wed, 05 Dec 2018 12:28:50 ofC. albicans INT1 (Gale et al., 1998),ALS1 (Fu et al., 1998b), ALA1 (Gaur & Klotz, 1997) or CAD1/AAF1 (Fu et al., 1998a) genes in S. cerevisiae is sufficient to direct the adhesion of this normally non-adherent yeast to huma…”
BackgroundInfluential
EAP1 , a Candida albicans Gene Involved in Binding Human Epithelial Cells
Li, F., & Palecek, S. P. (2003) · Eukaryotic Cell · doi:10.1128/ec.2.6.1266-1273.2003 · Cited by 189
Candida albicans Agglutinin-Like Sequence (Als) Family Vignettes: A Review of Als Protein Structure and Function
Hoyer, L. L., & Cota, E. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00280 · Cited by 229
“Additional effort revealed the full nature of the ALS family in C. albicans (Gaur and Klotz, 1997; Hoyer et al., 1998b; Hoyer and Hecht, 2000, 2001; Zhao et al., 2007a), which proved to be essential for accurate assembly of the C. albicans genome sequence Abbreviations: AFR, amyloid-forming region;…”
Background
RT-PCR detection of Candida albicans ALS gene expression in the reconstituted human epithelium (RHE) model of oral candidiasis and in model biofilms
Green, C. B., Cheng, G., Chandra, J., Mukherjee, P. K., Ghannoum, M. A., & Hoyer, L. L. (2004) · Microbiology · doi:10.1099/mic.0.26699-0 · Cited by 159
“Among these is the ALS (agglutinin-like sequence) family that encodes cell-wall glycoproteins, some of which are involved in adherence to host surfaces ( Gaur & Klotz, 1997; Hoyer, 2001; Fu et al., 2002).”
Background
Aspects of Fungal Pathogenesis in Humans
van Burik, J. H., & Magee, P. T. (2001) · Annual Review of Microbiology · doi:10.1146/annurev.micro.55.1.743 · Cited by 176
Candida albicans-epithelial interactions and pathogenicity mechanisms: scratching the surface
Moyes, D. L., Richardson, J. P., & Naglik, J. R. (2015) · Virulence · doi:10.1080/21505594.2015.1012981 · Cited by 197
Analysis of the Candida albicans Als2p and Als4p adhesins suggests the potential for compensatory function within the Als family.
Zhao, X.-M., Oh, S.-H., Yeater, K., & Hoyer, L. (2005) · Microbiology · doi:10.1099/mic.0.27763-0 · Cited by 166
“Overproduction of other Als proteins in Saccharomyces cerevisiae produced an adherent phenotype for this normally non-adherent organism, suggesting they may also play a role in C. albicans adhesion (Gaur & Klotz, 1997; Sheppard et al., 2004).”
Background
Micromechanical cantilever array sensors for selective fungal immobilization and fast growth detection.
Nugaeva, N., Gfeller, K. Y., Backmann, N., Lang, H., Düggelin, M., & Hegner, M. (2005) · Biosensors & bioelectronics · doi:10.1016/j.bios.2005.02.004 · Cited by 164
“Pathogenic yeast and mycelial fungi adhere to Fn molecules at different sites: the 40-kDa GAG-binding domain, the 120-kDa domain and the RGD sequence ( Guar and Klotz, 1997; Lima et al., 2001).”
Background
Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells
Fu, Y., Rieg, G., Fonzi, W. A., Belanger, P. H., Edwards, J. E., & Filler, S. G. (1998) · Infection and Immunity · doi:10.1128/iai.66.4.1783-1786.1998 · Cited by 159
“Recently, Gaur and Klotz (4) described another member of the ALS gene family, ALA1.”
Background
Modular domain structure in the Candida glabrata adhesin Epa1p, a β1,6 glucan‐cross‐linked cell wall protein
Frieman, M. B., McCaffery, J. M., & Cormack, B. P. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.03166.x · Cited by 150
“A particularly well-characterized family of GPI-CWPs in C. albicans is encoded by the ALS genes (Hoyer, 2001), at least two members of which, ALS1 and ALA1, encode adhesins (Gaur and Klotz, 1997; Fu et al., 1998).”
BackgroundInfluential
Yeast Cell Adhesion Molecules Have Functional Amyloid-Forming Sequences
Ramsook, C. B., Tan, C., Garcia, M. C., Fung, R., Soybelman, G., Henry, R., Litewka, A., O'Meally, S., Otoo, H., Khalaf, R. A., et al. (2009) · Eukaryotic Cell · doi:10.1128/ec.00068-09 · Cited by 156
“Aggregation assays for Als adhesins were carried out as previously described (15).”
MethodSelf-citation
Adhesins in Candida albicans
Sundstrom, P. (1999) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(99)80062-9 · Cited by 134
Eap1p, an Adhesin That Mediates Candida albicans Biofilm Formation In Vitro and In Vivo
Li, F., Svarovsky, M. J., Karlsson, A. J., Wagner, J. P., Marchillo, K., Oshel, P. E., Andes, D. R., & Palecek, S. P. (2007) · Eukaryotic Cell · doi:10.1128/ec.00049-07 · Cited by 154
“albicans genes encoding GPI-CWP adhesins, including ALS1, ALS2, ALS4, ALS5 (ALA1), HWP1, and EAP1, mediate adhesion to organic and inorganic surfaces, extracellular matrix proteins, human endothelial cells, and epithelial cells (15, 17, 32, 51, 61).”
Background
Glycan microarray analysis of Candida glabrata adhesin ligand specificity
Zupancic, M. L., Frieman, M., Smith, D., Alvarez, R. A., Cummings, R. D., & Cormack, B. P. (2008) · Molecular Microbiology · doi:10.1111/j.1365-2958.2008.06184.x · Cited by 143
“…of several of the Als proteins have been characterized – for example,Als1,Als3 andAls5 have been found to adhere, to varying degrees, to both epithelial and endothelial cell types, as well as to extracellular matrix proteins (Gaur and Klotz, 1997; Fu et al., 1998; Sheppard et al., 2004).”
Background
Candida albicans Als Adhesins Have Conserved Amyloid-Forming Sequences
Otoo, H., Lee, K., Qiu, W.-G., & Lipke, P. (2007) · Eukaryotic Cell · doi:10.1128/ec.00309-07 · Cited by 139
“These cell wall-bound adhesins bind to diverse mammalian peptide ligands, cause cellular aggregation through homotypic adhesion (8, 16, 17), and also coaggregate with other microbial pathogens to mediate polymicrobial infections (15).”
Background
Adherence mechanisms in human pathogenic fungi
Tronchin, G., Pihet, M., Lopes-Bezerra, L. M., & Bouchara, J. (2008) · Medical Mycology · doi:10.1080/13693780802206435 · Cited by 139
A Role for Amyloid in Cell Aggregation and Biofilm Formation
Garcia, M. C., Lee, J. T., Ramsook, C. B., Alsteens, D., Dufrêne, Y. F., & Lipke, P. N. (2011) · PLoS ONE · doi:10.1371/journal.pone.0017632 · Cited by 128
“Generation of V5-tagged Als5p and Als5p V5-tagged Als5p was generated by restriction digestion of pGK114 with BamHI and XhoI which released the Als5p sequence from the vector backbone [39].”
BackgroundMethodResult
Candida albicans Als proteins mediate aggregation with bacteria and yeasts.
Klotz, S., Gaur, N., Armond, R. L. D., Sheppard, D., Khardori, N., Edwards, J., Lipke, P., & El-azizi, M. (2007) · Medical Mycology · doi:10.1080/13693780701299333 · Cited by 125
Self-citation
Medicinal genetics approach towards identifying the molecular target of a novel inhibitor of fungal cell wall assembly
Tsukahara, K., Hata, K., Nakamoto, K., Sagane, K., Watanabe, N.-A., Kuromitsu, J., Kai, J.-K., Tsuchiya, M., Ohba, F., Jigami, Y., et al. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03481.x · Cited by 117
“These mannoproteins comprise a family that is termed fungal adhesins, which include Ala1p, Als1p and Hwp1p in C. albicans , and Epa1p in Candida glabrata (Hoyer et al ., 1995; Gaur and Klotz, 1997; Cormack et al ., 1999; Staab et al ., 1999).”
Background
Threonine-Rich Repeats Increase Fibronectin Binding in the Candidaalbicans Adhesin Als5p
Rauceo, J. M., De Armond, R., Otoo, H., Kahn, P. C., Klotz, S. A., Gaur, N. K., & Lipke, P. N. (2006) · Eukaryotic Cell · doi:10.1128/ec.00120-06 · Cited by 115
“The TR region of Als5p consists of six copies of a 36-mer repeat that is rich in Thr and other -branched amino acids (8).”
BackgroundSelf-citation
Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain
Oh, S.-H., Cheng, G., Nuessen, J. A., Jajko, R., Yeater, K. M., Zhao, X., Pujol, C., Soll, D. R., & Hoyer, L. L. (2005) · Microbiology · doi:10.1099/mic.0.27680-0 · Cited by 108
“The ALS (agglutinin-like sequence) gene family of Candida albicans encodes eight large cell-surface glycoproteins, some of which function in adhesion to host cells ( Gaur & Klotz, 1997; Hoyer, 2001; Fu et al., 2002; Zhao et al., 2004).”
Background
Improved efficacy of antifungal drugs in combination with monoterpene phenols against Candida auris
Shaban, S., Patel, M., & Ahmad, A. (2020) · Scientific Reports · doi:10.1038/s41598-020-58203-3 · Cited by 107
Comparison between Candida albicans Agglutinin-Like Sequence Gene Expression Patterns in Human Clinical Specimens and Models of Vaginal Candidiasis
Cheng, G., Wozniak, K. L., Wallig, M. A., Fidel, P. L., Trupin, S. R., & Hoyer, L. L. (2005) · Infection and Immunity · doi:10.1128/iai.73.3.1656-1663.2005 · Cited by 93
“The eight ALS genes (ALS1 to ALS7 and ALS9) encode large, cell surface glycoproteins, some of which promote adhesion to host surfaces (6, 7, 10, 35, 36).”
Background
N-Glycosylation of yeast, with emphasis onCandida albicans
Cutler, J. E. (2001) · Medical Mycology · doi:10.1080/mmy.39.1.75.86 · Cited by 76
Strengthening relationships: amyloids create adhesion nanodomains in yeasts
Lipke, P. N., Garcia, M. C., Alsteens, D., Ramsook, C. B., Klotz, S. A., & Dufrêne, Y. F. (2012) · Trends in Microbiology · doi:10.1016/j.tim.2011.10.002 · Cited by 104
“Each TR domain is a 36 residue βsheet fold with large surface-exposed regions of aliphatic and aromatic amino acid side chains [24, 38-40].”
BackgroundInfluentialSelf-citation
Candida albicans Interactions with Mucosal Surfaces during Health and Disease
Nikou, S.-A., Kichik, N., Brown, R., Ponde, N., Ho, J., Naglik, J., & Richardson, J. (2019) · Pathogens · doi:10.3390/pathogens8020053 · Cited by 103
Identification of Candida albicans ALS2 and ALS4 and Localization of Als Proteins to the Fungal Cell Surface
Hoyer, L. L., Payne, T. L., & Hecht, J. E. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.20.5334-5343.1998 · Cited by 103
“Accession numbers for other sequences discussed here include L25902 (ALS1 [26]), U87856 (ALS3 [25]), and AF025429 (ALA1/ALS5 [18]).”
Background
The ω‐site sequence of glycosylphosphatidylinositol‐anchored proteins in Saccharomyces cerevisiae can determine distribution between the membrane and the cell wall
Frieman, M., & Cormack, B. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03722.x · Cited by 98
Unfolding Individual Als5p Adhesion Proteins on Live Cells
Alsteens, D., Duprès, V., Klotz, S. A., Gaur, N. K., Lipke, P. N., & Dufrêne, Y. F. (2009) · ACS Nano · doi:10.1021/nn900078p · Cited by 92
“The protocol that we used for bead adherence assays is similar to that described elsewhere.(10,12) Briefly, Als5p-expressing cells were mixed with fibronectin-coated magnetic beads at a cell-to-bead ratio of 100:1 in Tris-EDTA buffer, vortexed, and incubated at room temperature with gentle shaking for 30 to 45 min.”
BackgroundMethodSelf-citation
Distinct domains of the Candida albicans adhesin Eap1p mediate cell–cell and cell–substrate interactions
Li, F., & Palecek, S. P. (2008) · Microbiology · doi:10.1099/mic.0.2007/013789-0 · Cited by 91
“Many of these proteins are involved in mediating the adhesion of C. albicans cells to host materials and/or inert surfaces (Fu et al., 2002; Gaur & Klotz, 1997; Phan et al., 2007; Staab et al., 1999; Zhao et al., 2005, 2007).”
Background
Expression and Characterization of the Flocculin Flo11/Muc1, a Saccharomyces cerevisiae Mannoprotein with Homotypic Properties of Adhesion
Douglas, L., Li, L., Yang, Y., & Dranginis, A. (2007) · Eukaryotic Cell · doi:10.1128/ec.00284-06 · Cited by 94
“For example, Gaur and Klotz used microscopic magnetic beads coated with extracellular matrix proteins to isolate a C. albicans adhesin gene, ALS5, by expression cloning in S. cerevisiae ( 13 ).”
Flocculation protein structure and cell–cell adhesion mechanism in Saccharomyces cerevisiae
Goossens, K. V. Y., & Willaert, R. (2010) · Biotechnology Letters · doi:10.1007/s10529-010-0352-3 · Cited by 92
“2003), inert surfaces such as agar and plastic (Gaur and Klotz 1997; Lo and Dranginis 1998; Reynolds and Fink 2001) and mammalian tissues (Cormack et al.”
Background
Structural basis for the broad specificity to host-cell ligands by the pathogenic fungus Candida albicans
Salgado, P. S., Yan, R., Taylor, J. D., Burchell, L., Jones, R., Hoyer, L. L., Matthews, S., Simpson, P. J., & Cota, E. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1103496108 · Cited by 85
“Data were processed with NMRPipe (6) and analyzed with NMRview (7).”
BackgroundMethod
Overexpression of theCandida albicans ALA1Gene inSaccharomyces cerevisiaeResults in Aggregation following Attachment of Yeast Cells to Extracellular Matrix Proteins, Adherence Properties Similar to Those ofCandida albicans
Gaur, N. K., Klotz, S. A., & Henderson, R. L. (1999) · Infection and Immunity · doi:10.1128/iai.67.11.6040-6047.1999 · Cited by 84
“cerevisiae transformants for ECM-coated magnetic beads (9), and Fu et al.”
BackgroundResultInfluentialSelf-citation
Degenerate Peptide Recognition by Candida albicans Adhesins Als5p and Als1p
Klotz, S., Gaur, N., Lake, D., Chan, V., Rauceo, J. M., & Lipke, P. (2004) · Infection and Immunity · doi:10.1128/iai.72.4.2029-2034.2004 · Cited by 89
“this adherence assay; all are equivalent (6), and the adherence of the fungal cells”
BackgroundSelf-citation
Vaccination with recombinant N-terminal domain of Als1p improves survival during murine disseminated candidiasis by enhancing cell-mediated, not humoral, immunity
Ibrahim, A. S., Spellberg, B. J., Avenissian, V., Fu, Y., Filler, S. G., & Edwards, J. E. (2005) · Infection and Immunity · doi:10.1128/iai.73.2.999-1005.2005 · Cited by 83
“We and others have determined that Als proteins function as adhesins to biologically relevant substrates (13, 15 , 32, 54).”
Background
What We Do Not Know about Fungal Cell Adhesion Molecules
Lipke, P. N. (2018) · Journal of Fungi · doi:10.3390/jof4020059 · Cited by 99
“cerevisiae has been successfully used for over a decade [22,73,105].”
BackgroundMethod
Flocculation of hyphae is associated with a deletion in the putative CaHK1 two-component histidine kinase gene from Candida albicans
Calera, J. A., & Calderone, R. (1999) · Microbiology · doi:10.1099/13500872-145-6-1431 · Cited by 80
Candida Species: New Insights into Biofilm Formation
Cuéllar-Cruz, M., López-Romero, E., Villagómez-Castro, J. C., & Ruiz-Baca, E. (2012) · Future Microbiology · doi:10.2217/fmb.12.48 · Cited by 80
“In order to adhere, the Als proteins interact with amino acid residues (Ser/ Thr) of different host ligands such as laminin, fibronectin and collagen [101].”
Background
Proteomic analysis of Candida albicans yeast and hyphal cell wall and associated proteins
Ebanks, R. O., Chisholm, K. A., McKinnon, S., Whiteway, M. S., & Pinto, D. M. (2006) · PROTEOMICS · doi:10.1002/pmic.200500100 · Cited by 78
Contribution of Candida albicans ALS1 to the Pathogenesis of Experimental Oropharyngeal Candidiasis
Kamai, Y., Kubota, M., Kamai, Y., Hosokawa, T., Fukuoka, T., & Filler, S. (2002) · Infection and Immunity · doi:10.1128/iai.70.9.5256-5258.2002 · Cited by 80
“Several adherence genes have been characterized (6, 7, 12).”
Background
Fungal invasion of epithelial cells
Yang, W., Yan, L., Wu, C., Zhao, X., & Tang, J. (2014) · Microbiological Research · doi:10.1016/j.micres.2014.02.013 · Cited by 79
Sixty Alleles of the ALS7 Open Reading Frame in Candida albicans: ALS7 Is a Hypermutable Contingency LocusPreprint
Zhang, N., Harrex, A. L., Holland, B. R., Fenton, L. E., Cannon, R. D., & Schmid, J. (2003) · Genome Research · doi:10.1101/gr.1024903 · Cited by 76
“…between GPG cluster strains and other strains to identify physiological and genetic traits which are associated with their increased pathogenicity, as a means to identify pathogenicity factors relevant to interactions of C. albicanswith the human host (Schmid et al. 1995; Giblin et al. 2001).”
BackgroundMethod
Allelic variation in the contiguous loci encoding Candida albicans ALS5, ALS1 and ALS9
Zhao, X., Pujol, C., Soll, D. R., & Hoyer, L. L. (2003) · Microbiology · doi:10.1099/mic.0.26495-0 · Cited by 74
“Ironically, the coding sequence immediately downstream of ALS9 was ALA1 .I n this case, ALA1 was not a synonym for ALS5 ( Gaur & Klotz, 1997 ), but encoded alanyl-tRNA synthetase.”
Background
Expression of a cytoplasmic transhydrogenase in Saccharomyces cerevisiae results in formation of 2‐oxoglutarate due to depletion of the NADPH pool
Nissen, T. L., Anderlund, M., Nielsen, J., Villadsen, J., & Kielland‐Brandt, M. C. (2000) · Yeast · doi:10.1002/1097-0061(200101)18:1 · Cited by 125
Candida albicans : adherence, signaling and virulence
Calderone, R., Suzuki, S., Cannon, R. D., Cho, T., Boyd, D., Calera, J. A., Chibana, H., Herman, D., Holmes, A. R., Jeng, H.-L., et al. (2000) · Medical Mycology · doi:10.1080/mmy.38.s1.125.137 · Cited by 60
The O-Mannosyltransferase PMT4 Is Essential for Normal Appressorium Formation and Penetration in Ustilago maydis[W][OA]
Fernández-Álvarez, A., Elías-Villalobos, A., & Ibeas, J. (2009) · The Plant Cell Online · doi:10.1105/tpc.109.065839 · Cited by 71
“Cell wall glycoproteins have been identified as fungal adhesives and have been implicated in host cell adhesion in many organisms (Gaur and Klotz, 1997; Buck and Andrews, 1999; Frieman et al., 2002; Sheppard et al., 2004).”
Background
Interaction of pathogenic fungi with host cells: Molecular and cellular approaches
Mendes‐Giannini, M. J. S., Soares, C. P., da Silva, J. L. M., & Andreotti, P. F. (2005) · FEMS Immunology & Medical Microbiology · doi:10.1016/j.femsim.2005.05.014 · Cited by 77
“The latter was detected only in a cytoplasmic extract and it may be a precursor of the former [46].”
Background
Construction and real-time RT-PCR validation of Candida albicans PALS-GFP reporter strains and their use in flow cytometry analysis of ALS gene expression in budding and filamenting cells
Green, C. B., Zhao, X., Yeater, K. M., & Hoyer, L. L. (2005) · Microbiology · doi:10.1099/mic.0.27696-0 · Cited by 64
“One example is the ALS (agglutinin-like sequence) gene family that encodes cell-surface glycoproteins involved in adhesion to host surfaces and potentially other cellular processes ( Gaur K Hoyer,2001;Fuetal.,2002;Zhaoetal., 2004; Sheppard et al., 2004).”
Background
Fungal allergy and pathogenicity
Breitenbach, M., Crameri, R., & Lehrer, S. (2002) · doi:10.1590/s0036-46652002000600012 · Cited by 68
Molecular and cellular mechanisms for microbial entry into the CNS.
Zhang, J.-R., & Tuomanen, E. (1999) · Journal of Neurovirology · doi:10.3109/13550289909021288 · Cited by 64
“Other C. albicans proteins such as ALA1 (Gaur and Klotz, 1997) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Gozalbo et al, 1998) have been reported to mediate adherence of the fungus to various extracellular matrix proteins.”
Background
Identification and characterization of a laminin-binding protein of Aspergillus fumigatus: extracellular thaumatin domain protein (AfCalAp).
Upadhyay, S., Mahajan, L., Ramjee, S., Singh, Y., Basir, S., & Madan, T. (2009) · Journal of Medical Microbiology · doi:10.1099/jmm.0.005991-0 · Cited by 63
“ECM proteins of the host, namely fibronectin, laminin, collagens and proteoglycans, are implicated in attachment of a number of fungal pathogens (Gaur & Klotz, 1997; Gil et al., 1996; Gonzalez et al., 2005; Lopez-Ribot & Chaffin, 1994; Mendes-Giannini et al., 2006; Tronchin et al., 1997; Wasylnka &…”
Background
RGD-mediated adhesion in fungal pathogens of humans, plants and insects.
Hostetter, M. K. (2000) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(00)00101-6 · Cited by 63
Functional regions of Candida albicans hyphal cell wall protein Als3 that determine interaction with the oral bacterium Streptococcus gordonii
Bamford, C. V., Nobbs, A. H., Barbour, M. E., Lamont, R. J., & Jenkinson, H. F. (2014) · Microbiology · doi:10.1099/mic.0.083378-0 · Cited by 61
“…signal peptide, an immunoglobulin (Ig)-like region (~300 aa), a conserved Thr-rich region, a central Thr-rich repeat domain (~470 aa residues), a Ser/Thr-rich C-terminal domain and a Cterminal glycosylphosphatidylinositol modification (Gaur & Klotz, 1997; Sheppard et al., 2004; Phan et al., 2007).”
Background
Study of Candida albicans and its interactions with the host: A mini review
Tsai, P.-W., Chen, Y.-T., Hsu, P., & Lan, C. (2013) · Biomedicine · doi:10.1016/j.biomed.2012.12.004 · Cited by 53
“, fibronectin, laminin, and type IV collagen) and buccal and pharyngeal epithelial and vascular endothelial cells [77,78].”
Background
Differential invasion of Candida albicans isolates in an in vitro model of oral candidosis.
Bartie, K., Williams, D., Wilson, M., Potts, A. J. C., & Lewis, M. (2004) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2004.00155.x · Cited by 61
Analysis ofPRA1and Its Relationship toCandida albicans- Macrophage Interactions
Marcil, A., Gadoury, C., Ash, J., Zhang, J., Nantel, A., & Whiteway, M. S. (2008) · Infection and Immunity · doi:10.1128/iai.00588-07 · Cited by 57
“Overall, Candida albicans surface proteins can bind many host proteins, including fibrinogen (10), complement fragments (34), plasminogen (13), and extracellular matrix proteins such as collagen, fibronectin, and laminin (28).”
Background
A Genetic Code Alteration Is a Phenotype Diversity Generator in the Human Pathogen Candida albicans
Miranda, I., Rocha, R., Santos, M. C., Mateus, D. D., Moura, G. R., Carreto, L., & Santos, M. A. S. (2007) · PLoS ONE · doi:10.1371/journal.pone.0000996 · Cited by 59
“serines with leucines at CUG positions increases adhesion [36–41].”
Background
Purification and Partial Characterization of a Paracoccidioides brasiliensis Protein with Capacity To Bind to Extracellular Matrix Proteins
González, Á., Gómez, B., Díez, S., Hernández, O., Restrepo, A., Hamilton, A., & Cano, L. E. (2005) · Infection and Immunity · doi:10.1128/iai.73.4.2486-2495.2005 · Cited by 58
“Several fungi of clinical importance such as Candida albicans (10, 15, 16, 31); Aspergillus fumigatus (4, 11, 18, 37, 49); Histoplasma capsulatum (33); Cryptococcus neoformans (41); Pneumocystis carinii (27, 34); Sporothrix schenckii (29); and Penicillium marneffei (22, 23) are known to attach to ECM proteins.”
Background
Recognition of Fibronectin by Penicillium marneffei Conidia via a Sialic Acid-Dependent Process and Its Relationship to the Interaction Between Conidia and Laminin
Hamilton, A., Jeavons, L., Youngchim, S., & Vanittanakom, N. (1999) · Infection and Immunity · doi:10.1128/iai.67.10.5200-5205.1999 · Cited by 56
“The short peptides (RGD and YIGSR) had no detectable effect on the interaction between conidia and fibronectin.”
BackgroundInfluential
Amyloid-Like β-Aggregates as Force-Sensitive Switches in Fungal Biofilms and Infections
Lipke, P. N., Klotz, S. A., Dufrêne, Y. F., Jackson, D. N., & Garcia, M. C. (2017) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00035-17 · Cited by 64
“albicans Als5p in laboratory strains of Saccharomyces cerevisiae leads to the cells becoming adherent and aggregative (63, 68).”
BackgroundSelf-citation
Integrin-like proteins in Candida spp. and other microorganisms.
Hostetter, M. K. (1999) · Fungal Genetics and Biology · doi:10.1006/fgbi.1999.1165 · Cited by 55
Targeting the GPI biosynthetic pathway
Yadav, U., & Khan, M. A. (2018) · Pathogens and Global Health · doi:10.1080/20477724.2018.1442764 · Cited by 55
LL37 and hBD-3 elevate the β-1,3-exoglucanase activity of Candida albicans Xog1p, resulting in reduced fungal adhesion to plastic.
Chang, H.-T., Tsai, P.-W., Huang, H.-H., Liu, Y.-S., Chien, T.-S., & Lan, C.-Y. (2012) · Biochemical Journal · doi:10.1042/bj20111454 · Cited by 52
Analysis of ALS5 and ALS6 allelic variability in a geographically diverse collection of Candida albicans isolates.
Zhao, X.-M., Oh, S.-H., Jajko, R., Diekema, D., Pfaller, M., Pujol, C., Soll, D., & Hoyer, L. (2007) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2007.05.004 · Cited by 49
“Genomic DNA from generation zero and generation 3000 was amplified by PCR to determine the number of copies of the tandem repeat sequence as described above for ALS5 and ALS6.”
BackgroundInfluential
Unequal contribution of ALS9 alleles to adhesion between Candida albicans and human vascular endothelial cells
Zhao, X., Oh, S.-H., & Hoyer, L. L. (2007) · Microbiology · doi:10.1099/mic.0.2006/005017-0 · Cited by 46
“Isolation of ALS1 and ALS5 based on their ability to confer adhesive function on the relatively non-adherent S. cerevisiae supported this hypothesis ( Gaur & Klotz, 1997; Fu et al., 1998).”
Background
The Beer Brewing Process: Wort Production and Beer Fermentation
Willaert, R. (2006) · doi:10.1002/9780470113554.ch20 · Cited by 48
Fungal immunity and pathogenesis in mammals versus the invertebrate model organismGalleria mellonella
Smith, D. F. Q., & Casadevall, A. (2021) · Pathogens and Disease · doi:10.1093/femspd/ftab013 · Cited by 48
Interaction of α-Agglutinin and a -Agglutinin, Saccharomyces cerevisiae Sexual Cell Adhesion Molecules
Zhao, H. F., Shen, Z., Kahn, P. C., & Lipke, P. N. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.9.2874-2880.2001 · Cited by 53
Secreted Proteinases and Other Virulence Mechanisms of Candida albicans
Monod, M., & Zepelin, M. B. (2002) · KARGER eBooks · doi:10.1159/000058865 · Cited by 44
Fluidic Force Microscopy Demonstrates That Homophilic Adhesion by Candida albicans Als Proteins Is Mediated by Amyloid Bonds between Cells
Dehullu, J., Valotteau, C., Herman-Bausier, P., Garcia-Sherman, M. C., Mittelviefhaus, M., Vorholt, J., Lipke, P., & Dufrêne, Y. (2019) · Nano letters (Print) · doi:10.1021/acs.nanolett.9b01010 · Cited by 46
Candida albicans and Saccharomyces cerevisiae Expressing ALA1/ALS5 Adhere to Accessible Threonine, Serine, or Alanine Patches
Gaur, N., Smith, R., & Klotz, S. (2002) · Cell Communication & Adhesion · doi:10.1080/15419060212187 · Cited by 46
“Ala1/Als5p-mediated adherence includes such properties as stability to shear forces, activity over broad pH optima, and resistance to blocking by many biological molecules, and it occurs within seconds of exposure of the fungus to the substrate (4, 5).”
BackgroundMethodResultInfluentialSelf-citation
Expression cloning of the Candida albicans CSA1 gene encoding a mycelial surface antigen by sorting of Saccharomyces cerevisiae transformants with monoclonal antibody‐coated magnetic beads
Lamarre, C., Deslauriers, N., & Bourbonnais, Y. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.01715.x · Cited by 46
“During the course of this study, Gaur and Klotz (1997) reported the cloning of the C. albicans ALA1 gene through sorting of S. cerevisiae transformants with magnetic beads coated with extracellular matrix proteins.”
Background
Molecular Genetic and Genomic Approaches to the Study of Medically Important Fungi
Magee, P. T., Gale, C. A., Berman, J. G., & Davis, D. A. (2003) · Infection and Immunity · doi:10.1128/iai.71.5.2299-2309.2003 · Cited by 42
In vitro evaluation of virulence attributes of Candida spp. isolated from patients affected by diabetes mellitus
Manfredi, M., McCullough, M. J., Al‐Karaawi, Z. M., Vescovi, P., & Porter, S. R. (2006) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2006.00275.x · Cited by 37
Identification of Candida albicans Genes that Induce Saccharomyces cerevisiae Cell Adhesion and Morphogenesis
Li, F., & Palecek, S. P. (2005) · Biotechnology Progress · doi:10.1021/bp050236c · Cited by 32

Authors: Gaur, N. K., & Klotz, S. A.
Affiliations: United States Department of Veterans Affairs (US)
ORCID: Klotz, S A
Venue: Infection and Immunity
Publisher: American Society for Microbiology
Published: 1997-12
Volume: 65
Issue: 12
Pages: 5289-5294
ISSN: 0019-9567 (ISSN-L)
1098-5522
DOI: 10.1128/iai.65.12.5289-5294.1997
PMC: PMC175761
OpenAlex: W2119843708
Cited by: 182
References: 0
Citations by year: 2025: 1 · 2023: 2 · 2022: 1 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 4 · 2016: 5 · 2015: 3 · 2014: 13 · 2013: 5 · 2012: 7
FWCI: 2.73
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Infection and Immunity (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Health Sciences › Medicine › Infectious Diseases › Fungal Infections and Studies
Keywords: Candida albicans, adhesins, extracellular matrix proteins, fibronectin, type IV collagen, laminin, fungal adhesion, ALS1, tandem repeats, buccal epithelial cells, candidiasis, gene cloning
MeSH terms: Amino Acid Sequence, Candida albicans, Cell Adhesion, Cloning, Molecular, Fungal Proteins, Genes, Fungal, Humans, Molecular Sequence Data, Saccharomyces cerevisiae, Cell Adhesion Molecules, Extracellular Matrix Proteins, Sequence Analysis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Expression, cloning, and characterization of a Candida albicans gene, ALA1, that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Metadata

Record

About these checks