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SarA Positively Controls Bap-Dependent Biofilm Formation in Staphylococcus aureus

Trotonda, María Pilar, Manna, Adhar C., Cheung, Ambrose L., Lasa, Iñigo, Penadés, José R.·2005·Journal of Bacteriology
DOI 10.1128/jb.187.16.5790-5798.2005
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Abstract

The biofilm-associated protein Bap is a staphylococcal surface protein involved in biofilm formation. We investigated the influence of the global regulatory locus sarA on bap expression and Bap-dependent biofilm formation in three unrelated Staphylococcus aureus strains. The results showed that Bap-dependent biofilm formation was diminished in the sarA mutants by an agr -independent mechanism. Complementation studies using a sarA clone confirmed that the defect in biofilm formation was due to the sarA mutation. As expected, the diminished capacity to form biofilms in the sarA mutants correlated with the decreased presence of Bap in the bacterial surface. Using transcriptional fusion and Northern analysis data, we demonstrated that the sarA gene product acts as an activator of bap expression. Finally, the bap promoter was characterized and the transcriptional start point was mapped by the rapid amplification of cDNA ends technique. As expected, we showed that purified SarA protein binds specifically to the bap promoter, as determined by gel shift and DNase I footprinting assays. Based on the previous studies of others as well as our work demonstrating the role for SarA in icaADBC and bap expression (J. Valle, A. Toledo-Arana, C. Berasain, J. M. Ghigo, B. Amorena, J. R. Penades, and I. Lasa, Mol. Microbiol. 48:1075-1087), we propose that SarA is an essential regulator controlling biofilm formation in S. aureus .

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Metadata

Authors
Trotonda, M. P., Manna, A. C., Cheung, A. L., Lasa, I., & Penadés, J. R.
Affiliations
  • Departmento de Química, Bioquímica y Biología Molecular, Universidad Cardenal Herrera-C.E.U.
  • Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755
  • Instituto de Agrobiotecnología y Recursos Naturales and Departmento de Producción Agraria, Universidad Pública de Navarra-CSIC, Pamplona 31006, Spain
Venue
Journal of Bacteriology
Publisher
American Society for Microbiology
Published
2005-08-15
Volume
187
Issue
16
Pages
5790-5798
ISSN
  • 0021-9193
  • 1098-5530
DOI
10.1128/jb.187.16.5790-5798.2005
Cited by
92
References
0
Language
en
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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CoChat. (2026). Citation check: SarA Positively Controls Bap-Dependent Biofilm Formation in Staphylococcus aureus. https://app.cochat.ai/papers/10.1128/jb.187.16.5790-5798.2005
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Trotonda, M. P., Manna, A. C., Cheung, A. L., Lasa, I., & Penadés, J. R. (2005). SarA Positively Controls Bap-Dependent Biofilm Formation in Staphylococcus aureus. Journal of Bacteriology. https://doi.org/10.1128/jb.187.16.5790-5798.2005

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