Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 647

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Identification of a New Family of Tissue-Specific Basic Helix-Loop-Helix Proteins with a Two-Hybrid System

Open access
Hollenberg, Stanley M., Sternglanz, Rolf, Cheng, Pei Feng, Weintraub, Harold·1995·Molecular and Cellular Biology
DOI 10.1128/mcb.15.7.3813
Open this paper in CoChat — free account

Abstract

With modified two-hybrid technology, we have isolated a member of a new family of basic helix-loop-helix (bHLH) transcription factors. Thing1 (Th1) was identified in a screen of a mouse embryo cDNA library as a partner for the Drosophila E protein daughterless. RNA in situ hybridization and reverse transcriptase-PCR demonstrate a stage- and tissue-specific distribution for the expression of Th1. Although tissue specific, the expression pattern of Th1 is fairly complex. During development, Th1 mRNA is widely expressed in extraembryonic tissues, portions of the heart, autonomic ganglia, the gut, and pharyngeal arches. At embryonic day 7.5 (E7.5), extraembryonic derivatives show robust Th1 expression. By E8.5, expression in the embryonic heart becomes detectable. During the next 2 days of development, the signal also includes gut and pharyngeal arches. Predominant expression at E13.5 is in neural crest derivatives, especially the autonomic nervous system and adrenal medulla. Expression of Th1 persists in the adult, in which it is localized to the smooth muscle cells of the gut. In vitro, Th1 protein recognizes a set of DNA sites that are more degenerate than has been determined for other bHLH factors, indicating a reduced binding specificity. Transient transfection of NIH 3T3 cells with GAL4-Th1 fusions reveals a repression activity mediated by the Th1 bHLH domain. In combination, these properties define Th1 as a new bHLH protein with a unique set of properties.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1128/mcb.15.7.3813

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 2 references

Research works printed but not linked: 65

  1. 10.1016/S0022-2836(05)80360-2 · doi:10.1016/s0022-2836(05)80360-2 · Paper page
  2. Baer, R. 1993. TAL1, TAL2, and LYL1: a family of basic helix-loop-helix proteins implicated in T cell acute leukaemia. Semin. Cancer Biol. 4:341–347.
  3. Bartel, P., C. T. Chien, R. Sternglanz, and S. Fields. 1993. Elimination of false positives that arise in using the two-hybrid system. BioTechniques 14:920–924.
  4. Bartel, P. L., C. Chien, R. Sternglanz, and S. Fields. 1993. Using the two-hybrid system to detect protein-protein interactions, p. 153–179. In D. A. Hartley (ed.), Cellular interactions in development: a practical approach. IRL Press, Oxford. · DOI · Paper page
  5. 10.1073/pnas.88.3.869 · DOI
  6. 10.1016/0092-8674(90)90214-Y · DOI · Paper page
  7. 10.1128/mcb.13.9.5216-5224.1993 · doi:10.1128/mcb.13.9.5216-5224.1993 · Paper page
  8. 10.1126/science.2174572 · DOI · Paper page
  9. 10.1038/346663a0 · DOI
  10. 10.1128/mcb.14.2.1245-1255.1994 · doi:10.1128/mcb.14.2.1245-1255.1994
  11. 10.1073/pnas.89.18.8492 · DOI
  12. 10.1002/neu.480241005 · DOI
  13. 10.1016/0168-9525(92)90099-P · DOI · Paper page
  14. 10.1002/j.1460-2075.1990.tb08126.x · DOI · Paper page
  15. 10.1073/pnas.88.21.9578 · DOI · Paper page
  16. 10.1073/pnas.88.5.1815 · DOI · Paper page
  17. 10.1016/0968-0004(94)90172-4 · DOI · Paper page
  18. 10.1073/pnas.89.2.599 · DOI · Paper page
  19. 10.1101/gad.3.5.628 · DOI · Paper page
  20. 10.1101/gad.8.8.970 · DOI · Paper page
  21. 10.1038/363038a0 · DOI · Paper page
  22. 10.1038/340245a0 · DOI · Paper page
  23. 10.1016/0168-9525(90)90012-U · DOI · Paper page
  24. 10.1073/pnas.85.23.8998 · DOI · Paper page
  25. Gobel, V., S. Lipkowitz, C. A. Kozak, and I. R. Kirsch. 1992. NSCL-2: a basic domain helix-loop-helix gene expressed in early neurogenesis. Cell Growth Differ. 3:143–148.
  26. 10.1128/mcb.12.7.3006-3014.1992 · doi:10.1128/mcb.12.7.3006-3014.1992
  27. Green, A. R., T. Lints, J. Visvader, R. Harvey, and C. G. Begley. 1992. SCL is coexpressed with GATA-1 in hemopoietic cells but is also expressed in developing brain. Oncogene 7:653–660.
  28. 10.1126/science.257.5073.1089 · DOI · Paper page
  29. 10.1093/nar/19.20.5791 · DOI · Paper page
  30. 10.1073/pnas.90.17.8028 · DOI · Paper page
  31. 10.1016/0092-8674(89)90612-0 · DOI · Paper page
  32. 10.1128/mcb.14.2.1256-1265.1994 · DOI
  33. Hwang, L. Y., M. Siegelman, L. Davis, N. Oppenheimer-Marks, and R. Baer. 1993. Expression of the TAL1 proto-oncogene in cultured endothelial cells and blood vessels of the spleen. Oncogene 8:3043–3046.
  34. 10.1016/0092-8674(87)90636-2 · DOI
  35. 10.1111/j.1432-1033.1993.tb18075.x · DOI
  36. 10.1182/blood.V83.5.1200.1200 · DOI · Paper page
  37. 10.1016/0012-1606(87)90351-4 · DOI
  38. Lee J. S. M. Hollenberg L. Snider D. Turner N. Lipnick and H. Weintraub. NeuroD a new basic helix-loop-helix protein can convert Xenopus ectoderm into neurons. Science in press.
  39. 10.1038/338039a0 · DOI · Paper page
  40. 10.1016/0092-8674(94)90159-7 · DOI · Paper page
  41. 10.1016/0092-8674(89)90682-X · DOI · Paper page
  42. 10.1101/gad.8.1.1 · DOI · Paper page
  43. 10.1073/pnas.88.5.1943 · DOI · Paper page
  44. 10.1073/pnas.91.15.7066 · DOI
  45. 10.1128/mcb.13.2.792-800.1993 · DOI
  46. 10.1073/pnas.90.16.7583 · DOI
  47. 10.1016/0092-8674(91)90545-A · DOI · Paper page
  48. 10.1093/nar/17.18.7539 · DOI · Paper page
  49. 10.1007/BF00340712 · DOI · Paper page
  50. Seuss, D. 1957. The cat in the hat. Houghton Mifflin, Boston.
  51. 10.1128/mcb.6.12.4763-4766.1986 · doi:10.1128/mcb.6.12.4763-4766.1986
  52. 10.1016/S0021-9258(18)53440-2 · DOI
  53. 10.1016/0092-8674(91)90653-G · DOI · Paper page
  54. 10.1128/mcb.11.11.5603-5611.1991 · doi:10.1128/mcb.11.11.5603-5611.1991 · Paper page
  55. 10.1073/pnas.90.14.6483 · DOI
  56. 10.1073/pnas.89.19.9010 · DOI
  57. 10.1016/0968-0004(91)90137-K · DOI
  58. 10.1016/0092-8674(93)90307-C · DOI · Paper page
  59. 10.1073/pnas.88.24.11505 · DOI · Paper page
  60. 10.1073/pnas.87.15.5623 · DOI · Paper page
  61. 10.1016/0012-1606(91)90086-I · DOI · Paper page
  62. 10.1128/mcb.11.8.4104-4110.1991 · doi:10.1128/mcb.11.8.4104-4110.1991 · Paper page
  63. 10.1016/0092-8674(89)90583-7 · DOI · Paper page
  64. 10.1073/pnas.88.24.11416 · DOI · Paper page
  65. 10.1073/pnas.86.20.7989 · DOI

Cited by

See all 647

Metadata

Authors
Hollenberg, S. M., Sternglanz, R., Cheng, P. F., & Weintraub, H.
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
1995-07
Volume
15
Issue
7
Pages
3813-3822
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.15.7.3813
OpenAlex
W2168545181
Cited by
647
References
2
FWCI
15.87
Type
journal article
Language
en
Open access
green
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Identification of a New Family of Tissue-Specific Basic Helix-Loop-Helix Proteins with a Two-Hybrid System. https://app.cochat.ai/papers/10.1128/mcb.15.7.3813
The paper
Hollenberg, S. M., Sternglanz, R., Cheng, P. F., & Weintraub, H. (1995). Identification of a New Family of Tissue-Specific Basic Helix-Loop-Helix Proteins with a Two-Hybrid System. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.15.7.3813

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.