Cited by 161Fungal and yeast genetics researchProtein Kinase Regulation and GTPase SignalingBacterial Genetics and Biotechnology

Signaling in the Yeast Pheromone Response Pathway: Specific and High-Affinity Interaction of the Mitogen-Activated Protein (MAP) Kinases Kss1 and Fus3 with the Upstream MAP Kinase Kinase Ste7

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Bardwell, Lee, Cook, Jeanette G., Chang, Ernie C., Cairns, Bradley R., Thorner, Jeremy·1996·Molecular and Cellular Biology

Abstract

Kss1 and Fus3 are mitogen-activated protein kinases (MAPKs or ERKs), and Ste7 is their activating MAPK/ERK kinase (MEK), in the pheromone response pathway of Saccharomyces cerevisiae. To investigate the potential role of specific interactions between these enzymes during signaling, their ability to associate with each other was examined both in solution and in vivo. When synthesized by in vitro translation, Kss1 and Fus3 could each form a tight complex (Kd of approximately 5 nM) with Ste7 in the absence of any additional yeast proteins. These complexes were specific because neither Hog1 nor Mpk1 (two other yeast MAPKs), nor mammalian Erk2, was able to associate detectably with Ste7. Neither the kinase catalytic core of Ste7 nor the phosphoacceptor regions of Ste7 and Kss1 were necessary for complex formation. Ste7-Kss1 (and Ste7-Fus3) complexes were present in yeast cell extracts and were undiminished in extracts prepared from a ste5delta-ste11delta double mutant strain. In Ste7-Kss1 (or Ste7-Fus3) complexes isolated from naive or pheromone-treated cells, Ste7 phosphorylated Kss1 (or Fus3), and Kss1 (or Fus3) phosphorylated Ste7, in a pheromone-stimulated manner; dissociation of the high-affinity complex was shown to be required for either phosphorylation event. Deletions of Ste7 in the region required for its stable association with Kss1 and Fus3 in vitro significantly decreased (but did not eliminate) signaling in vivo. These findings suggest that the high-affinity and active site-independent binding observed in vitro facilitates signal transduction in vivo and suggest further that MEK-MAPK interactions may utilize a double-selection mechanism to ensure fidelity in signal transmission and to insulate one signaling pathway from another.

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Crossref · 10.1128/mcb.16.7.3637

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Authors
Bardwell, L., Cook, J. G., Chang, E. C., Cairns, B. R., & Thorner, J.
Affiliations
  • University of California, Berkeley (US)
  • Stanford Medicine (US)
Venue
Molecular and Cellular Biology
Published
1996-07-01
Volume
16
Issue
7
Pages
3637-3650
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2099221935
Cited by
161
References
101
Citations by year
2025: 3 · 2024: 1 · 2023: 4 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 2 · 2014: 5 · 2013: 2 · 2012: 8
FWCI
5.06
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5f32gm015871-02, GM21841)
  • National Institute of General Medical Sciences (GM-07232, GM 21841, T32GM007232, GM15871)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
pheromone response pathway, Saccharomyces cerevisiae, Kss1, FUS3, Ste7, MAP kinase kinase, mitogen-activated protein kinase, protein-protein interaction, signal transduction, kinase phosphorylation, signal fidelity, Ste5, Ste11
MeSH terms
Base Sequence, Binding Sites, Cloning, Molecular, Escherichia coli, Fungal Proteins, Molecular Sequence Data, Oligodeoxyribonucleotides, Phenotype, Pheromones, Protein Binding, Protein Kinases, Recombinant Proteins, Saccharomyces cerevisiae, Transcription, Genetic, Restriction Mapping, Mutagenesis, Site-Directed, Calcium-Calmodulin-Dependent Protein Kinases, Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinase Kinases, Saccharomyces cerevisiae Proteins

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