The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
HuR Regulates p21 mRNA Stabilization by UV Light
Open accessAbstract
Expression of the cyclin-dependent kinase inhibitor p21 is highly induced by many stresses, including exposure to short-wavelength UV light (UVC), which increases p21 mRNA stability. Investigation into the mechanisms underlying this stabilization process revealed that proteins present in cytoplasmic lysates of human RKO colorectal carcinoma cells formed complexes with p21 mRNA that were inducible by treatment with UVC and other stress agents. The ubiquitous Elav-type RNA-binding protein HuR was identified within the p21 mRNA-protein complexes, as antibodies recognizing HuR supershifted these complexes and revealed HuR-immunoreactive proteins complexing with p21 mRNA on Western blots. Lowering of endogenous HuR levels through expression of antisense HuR decreased p21 RNA-protein complexes, greatly reduced the UVC inducibility and half-life of p21 mRNA, and prevented UVC-mediated induction of luciferase activity in p21 3' untranslated region-containing reporter constructs. Our findings indicate that HuR plays a major role in regulating stress-induced p21 expression by enhancing p21 mRNA stability and that these effects are coupled to HuR's elevated presence in the cytoplasm.
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Cites
Mice Lacking p21CIP1/WAF1 undergo normal development, but are defective in G1 checkpoint control
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AU-rich elements: characterization and importance in mRNA degradation
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Self-citationA conserved family of elav-like genes in vertebrates.
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Identification of HuR as a protein implicated in AUUUA‐mediated mRNA decay
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Brennan, C. M., & Steitz*, J. A. (2001) · Cellular and Molecular Life Sciences · doi:10.1007/pl00000854 · Cited by 1,095
AU-rich elements and associated factors: are there unifying principles?
Barreau, C. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki1012 · Cited by 907
Redox regulation of FoxO transcription factors
Klotz, L.-O., Sánchez-Ramos, C., Prieto-Arroyo, I., Urbánek, P., Steinbrenner, H., & Monsalve, M. (2015) · Redox Biology · doi:10.1016/j.redox.2015.06.019 · Cited by 747
MicroRNA regulation by RNA-binding proteins and its implications for cancer
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Diverse molecular functions of Hu proteins
Hinman, M. N., & Lou, H. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-008-8252-6 · Cited by 587
Metadata
- Authors
- Wang, W., Furneaux, H., Cheng, H., Caldwell, M. C., Hutter, D., Liu, Y., Holbrook, N., & Gorospe, M.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2000-02
- Volume
- 20
- Issue
- 3
- Pages
- 760-769
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2049462796
- Cited by
- 539
- References
- 8
- FWCI
- 9.91
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, W., Furneaux, H., Cheng, H., Caldwell, M. C., Hutter, D., Liu, Y., Holbrook, N., & Gorospe, M. (2000). HuR Regulates p21 mRNA Stabilization by UV Light. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.20.3.760-769.2000
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