Cited by 167Gastrointestinal motility and disordersInflammatory Bowel DiseaseMast cells and histamine

The DOI matches its Crossref record; 45 of the 47 references it cites were found in a registry or an index.

Phenotypic changes in colonocytes following acute stress or activation of mast cells in mice: implications for delayed epithelial barrier dysfunction

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Demaude, J·2006·Gut

Abstract

BACKGROUND AND AIMS: Stressful life events are known to modulate the development or relapse of disease in both inflammatory bowel disease and irritable bowel disease patients but underlying mechanisms remain unclear. Stress is known to effect mast cells, interferon gamma (IFN-gamma), and myosin light chain phosphorylation to trigger colonic epithelial barrier dysfunction. The aim of this study was to investigate whether acute stress induced or chemical mast cell activation impaired expression and function of epithelial tight junctions, and altered colonocyte differentiation in mice. METHODS: Colonic paracellular permeability was assessed as the in vivo lumen to blood ratio of 51Cr-EDTA in different groups of mice (controls, stressed, mast cell degranulator BrX-537A treated), pretreated or not with the mast cell stabiliser doxantrazole. Involvement of mast cells and IFN-gamma was evaluated in wild-type and IFN-gamma deficient mice. Tight junction alteration was assessed by histology, transmission electron microscopy, and real time reverse transcription-polymerase chain reaction. Colonocyte differentiation was determined by protein kinase C zeta (PKCzeta) immunofluorescence and western blotting, and alkaline phosphatase activity assay. RESULTS: Acute stress induced a three day delayed increase in colonic paracellular permeability which involved mast cell degranulation and overproduction of IFN-gamma. The colonic epithelial barrier was morphologically altered and expression of mRNA encoding tight junction proteins ZO-2 and occludin was decreased. Moreover, three days after acute stress, colonocyte differentiation was reduced, as shown by decreased expression of both PKCzeta isotype and alkaline phosphatase. CONCLUSION: These data highlight new mechanisms whereby an acute stress acts on the gastrointestinal tract by inducing alterations in colonocyte differentiation and decreased expression of mRNA encoding tight junction proteins. Thus phenotypic changes in colonocytes could pave the way for stress related intestinal disorders.

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Crossref · 10.1136/gut.2005.078675

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  1. [29]29. Metcalfe DD, Baram D, Mekori YA. Mast cells. Physiol Rev 1997;77:1033-79.

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Metadata

Authors
Demaude, J.
Affiliations
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
Corresponding author
Demaude, J
Venue
Gut
Publisher
BMJ
Published
2006-05-01
Volume
55
Issue
5
Pages
655-661
ISSN
0017-5749 (ISSN-L)
OpenAlex
W2119510464
Cited by
167
References
47
Citations by year
2026: 5 · 2025: 8 · 2024: 8 · 2023: 13 · 2022: 12 · 2021: 12 · 2020: 9 · 2019: 7 · 2018: 7 · 2017: 6 · 2016: 7 · 2015: 6 · 2014: 7 · 2013: 3 · 2012: 12
FWCI
4.36
Type
journal article
Language
en
Open access
Locations
  • Gut (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
  • Institut National de la Santé et de la Recherche Médicale
  • Institut National de la Recherche Agronomique
Topics
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Health Sciences › Medicine › Gastroenterology › Inflammatory Bowel Disease
  • Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
Keywords
acute stress, mast cell activation, mast cell degranulation, tight junction proteins, ZO-2, occludin, interferon-gamma, doxantrazole, paracellular permeability, protein kinase C zeta, alkaline phosphatase, irritable bowel disease, inflammatory bowel disease
MeSH terms
Alkaline Phosphatase, Animals, Cell Differentiation, Cell Membrane Permeability, Colon, Epithelial Cells, Interferon-gamma, Intestinal Mucosa, Male, Mast Cells, Membrane Proteins, Mice, Inbred Strains, Phosphorylation, Protein Kinase C, Stress, Psychological, Time Factors, Cell Degranulation, Mice, Knockout, Myosin Light Chains, Tight Junctions, Reverse Transcriptase Polymerase Chain Reaction, Microscopy, Electron, Transmission, Mice, Occludin, Zonula Occludens-2 Protein

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