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Cited by 375Gastrointestinal motility and disordersGut microbiota and healthFood composition and properties

Mucin secretion is modulated by luminal factors in the isolated vascularly perfused rat colon

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Barcelo, A, Claustre, J, Moro, F, Chayvialle, J-A, Cuber, J-C, Plaisancié, P·2000·Gut
DOI 10.1136/gut.46.2.218PMC PMC1727811
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Abstract

BACKGROUND: Mucins play an important protective role in the colonic mucosa. Luminal factors modulating colonic mucus release have been not fully identified. AIM: To determine the effect of some dietary compounds on mucus discharge in rat colon. METHODS: An isolated vascularly perfused rat colon model was used. Mucus secretion was induced by a variety of luminal factors administered as a bolus of 1 ml for 30 minutes in the colonic loop. Mucin release was evaluated using a sandwich enzyme linked immunosorbent assay supported by histological analysis. RESULTS: The three dietary fibres tested in this study (pectin, gum arabic, and cellulose) did not provoke mucus secretion. Luminal administration of sodium alginate (an algal polysaccharide used as a food additive) or ulvan (a sulphated algal polymer) induced a dose dependent increase in mucin discharge over the concentration range 1–25 mg/l (p<0.05 for 25 mg/l alginate and p<0.05 for 10 and 25 mg/l ulvan). Glucuronic acid and galacturonic acid, which are major constituents of a variety of fibres, produced significant mucin secretion (p<0.05). Hydrogen sulphide and mercaptoacetate, two sulphides produced in the colonic lumen by microbial fermentation of sulphated polysaccharides, did not modify mucin secretion. Among the short chain fatty acids, acetate (5–100 mM) induced a dose dependent release of mucus (p<0.05 for 100 mM acetate). Interestingly, butyrate at a concentration of 5 mM produced colonic mucin secretion (p<0.05), but increasing its concentration to 100 mM provoked a gradual decrease in mucus discharge. Propionate (5–100 mM) did not induce mucin release. Several dietary phenolic compounds (quercetin, epicatechin, resveratrol) did not provoke mucus discharge. CONCLUSIONS: Two algal polysaccharides (alginate and ulvan), two uronic acids (glucuronic acid and galacturonic acid), and the short chain fatty acids acetate and butyrate induce mucin secretion in rat colon. Taken together, these data suggest that some food constituents and their fermentation products may regulate the secretory function of colonic goblet cells.

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  • SCFAs can enhance the mucosal barrier through the stimulation of mucus production, which is likely mediated through FFAR3.

    “SCFAs can enhance the mucosal barrier through the stimulation of mucus production (Barcelo et al., 2000;Finnie et al., 1995;Jung et al., 2015;Willemsen et al., 2003), which is likely mediated through FFAR3 (Said et al., 2017).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Barcelo et al., 2000

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Authors
Barcelo, A., Claustre, J., Moro, F., Chayvialle, J.-A., Cuber, J.-C., & Plaisancié, P.
Affiliations
Inserm (FR)
Corresponding author
Plaisancié, P
Venue
Gut
Publisher
BMJ
Published
2000-02-01
Volume
46
Issue
2
Pages
218-224
ISSN
  • 0017-5749 (ISSN-L)
  • 1468-3288
DOI
10.1136/gut.46.2.218
PMC
PMC1727811
OpenAlex
W2106528813
Cited by
375
References
1
Citations by year
2026: 10 · 2025: 9 · 2024: 21 · 2023: 17 · 2022: 25 · 2021: 23 · 2020: 24 · 2019: 22 · 2018: 25 · 2017: 18 · 2016: 15 · 2015: 17 · 2014: 20 · 2013: 11 · 2012: 17
FWCI
5.35
Type
journal article
Language
en
Open access
Locations
  • Gut (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • HighWire Press Open Archive (submittedVersion)
Topics
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
Keywords
mucin secretion, colonic mucus, colonic goblet cells, dietary fiber, sodium alginate, ulvan, glucuronic acid, galacturonic acid, short-chain fatty acids, butyrate, acetate, algal polysaccharides, uronic acid, food additives, colonic fermentation, quercetin, epicatechin, resveratrol, mucosal protection
MeSH terms
Acetates, Alginates, Analysis of Variance, Animals, Butyrates, Cellulose, Colon, Dietary Fiber, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Food, Food Additives, Gum Arabic, Hexuronic Acids, Hydrogen Sulfide, Male, Mucins, Pectins, Perfusion, Polysaccharide-Lyases, Stimulation, Chemical, Rats, Wistar, Glucuronic Acid, Rats

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