Does calorie restriction induce mitochondrial biogenesis? A reevaluation
Open accessAbstract
It has been reported that 30% calorie restriction (CR) for 3 mo results in large increases in mitochondrial biogenesis in heart, brain, liver, and adipose tissue, with concomitant increases in respiration and ATP synthesis. We found these results surprising, and performed this study to determine whether 30% CR does induce an increase in mitochondria in heart, brain, liver, adipose tissue, and/or skeletal muscle. To this end, we measured the levels of a range of mitochondrial proteins, and mRNAs. With the exception of long-chain acyl-CoA dehydrogenase protein level, which was increased ∼60% in adipose tissue, none of the mitochondrial proteins or mRNAs that we measured were increased in rats subjected to 30% CR for 14 wk. There was also no increase in citrate synthase activity. Because it is not possible to have an increase in mitochondria without any increase in key mitochondrial proteins, we conclude that 30% CR does not induce an increase in mitochondria in heart, brain, liver, adipose tissue, or skeletal muscle in laboratory rodents.
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Long-term caloric restriction does not alter markers of mitochondrial biogenesis.
“Similarly, long-term CR did not alter any markers of mitochondrial biogenesis”
From Mitochondrial (Dys)function and Insulin Resistance: From Pathophysiological Molecular Mechanisms to the Impact of Diet (2019) · cites this paper as Hancock et al., 2011
Long-term caloric restriction prevents age-related loss of mitochondrial oxidative capacity and efficiency in isolated mitochondria and in muscle fibres.
“although CR prevented age-related loss of mitochondrial oxidative capacity and efficiency in isolated mitochondria and in muscle fibres”
From Mitochondrial (Dys)function and Insulin Resistance: From Pathophysiological Molecular Mechanisms to the Impact of Diet (2019) · cites this paper as Hancock et al., 2011
Hancock et al. could not detect changes in mRNA or protein levels of markers of mitochondrial biogenesis, or citrate synthase activity, in muscle from rats that underwent 30% caloric restriction for 14 weeks.
“Hancock et al. could not detect any changes in mRNA or protein levels of markers of mitochondrial biogenesis, or citrate synthase activity in muscle from rats that underwent 30% CR for 14 weeks (Hancock et al., 2011).”
From Mitochondrial (Dys)function and Insulin Resistance: From Pathophysiological Molecular Mechanisms to the Impact of Diet (2019) · cites this paper as Hancock et al., 2011
Long-term caloric restriction reduces oxidative damage.
“and reduced oxidative damage (Hancock et al., 2011).”
From Mitochondrial (Dys)function and Insulin Resistance: From Pathophysiological Molecular Mechanisms to the Impact of Diet (2019) · cites this paper as Hancock et al., 2011
The effects of caloric restriction on mitochondrial function are not consistent across studies or across tissues.
“However, this is not consistent across studies or across tissues (Hancock et al., 2011; Lanza et al., 2012; Walsh et al., 2014).”
From Mitochondrial (Dys)function and Insulin Resistance: From Pathophysiological Molecular Mechanisms to the Impact of Diet (2019) · cites this paper as Hancock et al., 2011
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- Authors
- Hancock, C. R., Han, D., Higashida, K., Kim, S. H., & Holloszy, J. O.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Holloszy, John O.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2010-11-03
- Volume
- 25
- Issue
- 2
- Pages
- 785-791
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- PMC
- PMC3023396
- OpenAlex
- W2058512647
- Cited by
- 131
- References
- 45
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 6 · 2021: 3 · 2020: 9 · 2019: 6 · 2018: 3 · 2017: 15 · 2016: 8 · 2015: 14 · 2014: 15 · 2013: 12 · 2012: 13
- FWCI
- 5.21
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The FASEB Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (AG00425)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- caloric restriction, mitochondrial biogenesis, mitochondrial proteins, mitochondrial mRNA, citrate synthase, long-chain acyl-CoA dehydrogenase, heart, brain, liver, adipose tissue, skeletal muscle, mitochondrial respiration, ATP production, rats
- MeSH terms
- Adipose Tissue, Animals, Brain, Gene Expression Regulation, Liver, Male, Mitochondria, Myocardium, RNA, Messenger, Rats, Wistar, Muscle, Skeletal, Mitochondrial Proteins, Caloric Restriction, Rats
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