Maternal obesity markedly increases placental fatty acid transporter expression and fetal blood triglycerides at midgestation in the ewe
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Obesity of women at conception is increasing, a condition associated with offspring obesity. We hypothesized that maternal obesity increases placental fatty acid transporter (FATP) expression, enhancing delivery of fatty acids to their fetuses. Sheep are a commonly utilized biomedical model for pregnancy studies. Nonpregnant ewes were randomly assigned to a control group [100% of National Research Council (NRC) recommendations] or obese group (OB, 150% of NRC) from 60 days before conception to 75 or 135 days of gestation (dG; term = 150 dG), when placental cotyledonary tissue was collected for analysis. Fetuses of OB ewes were markedly heavier ( P < 0.05) on 75 dG than fetuses from control ewes, but this difference disappeared by 135 dG. Maternal obesity markedly increased ( P < 0.05) cholesterol and triglyceride concentrations of both maternal and fetal blood. There is no difference in lipoprotein lipase mRNA expression between control and OB group at either gestational age. On 75 dG, the mRNA expression of FATP1 ( P < 0.05), FATP4 ( P = 0.08), and fatty acid translocase CD (cluster of differentiation) 36 ( P < 0.05) proteins were more enhanced in cotyledonary tissue from OB than control ewes; consistently, protein expression of FATP1 and FATP4 was increased ( P < 0.05). Similarly, on 135 dG, the mRNA levels of FATP1, FATP4, and CD36 were all higher ( P < 0.05), but only FATP4 protein content was enhanced ( P < 0.05) in OB cotyledonary tissue. Peroxisome proliferator-activated receptor (PPAR)-γ regulates the expression of FATPs. Both the mRNA expression and protein content of PPARγ were increased in OB cotyledonary in the midgestation. In conclusion, maternal obesity enhances the mRNA expression and protein content of FATPs in cotyledonary in the midgestation, which is associated with higher PPARγ content in cotyledonary.
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Diet-induced obesity of ewes increases expression of several fatty acid transporters in the placenta at both mid and late gestation.
“In contrast, diet-induced obesity of ewes increases expression of several fatty acid transporters in the placenta at both mid and late gestation (616).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [616]
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- Authors
- Zhu, M. J., Ma, Y., Long, N. M., Du, M., & Ford, S. P.
- Affiliations
- University of Wyoming (US)
- Corresponding author
- Zhu, Mei J.
- Venue
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Publisher
- American Physiological Society
- Published
- 2010-11
- Volume
- 299
- Issue
- 5
- Pages
- R1224-R1231
- ISSN
- 0363-6119 (ISSN-L)
- 1522-1490
- PMC
- PMC2980459
- OpenAlex
- W2137743034
- Cited by
- 137
- References
- 64
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 2 · 2023: 6 · 2022: 4 · 2021: 3 · 2020: 11 · 2019: 10 · 2018: 14 · 2017: 16 · 2016: 11 · 2015: 13 · 2014: 7 · 2013: 18 · 2012: 10
- FWCI
- 9.27
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (2p20rr016474-09)
- National Center for Research Resources (P20-RR-016474)
- Topics
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Reproductive Physiology in Livestock
- Keywords
- maternal obesity, fetal growth, FATP1, FATP4, CD36, PPARγ, placental cotyledons, lipoprotein lipase, fetal lipid metabolism
- MeSH terms
- Animals, Animal Nutritional Physiological Phenomena, Cholesterol, Female, Fetal Blood, Gene Expression Regulation, Gestational Age, Lipoprotein Lipase, Obesity, Placenta, Pregnancy, RNA, Messenger, Sheep, Triglycerides, CD36 Antigens, Fetal Weight, Maternal Nutritional Physiological Phenomena, PPAR gamma, p38 Mitogen-Activated Protein Kinases, Fatty Acid Transport Proteins
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