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Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
Open accessAbstract
The use of avidin-biotin interaction in immunoenzymatic techniques provides a simple and sensitive method to localize antigens in formalin-fixed tissues. Among the several staining procedures available, the ABC method, which involves an application of biotin-labeled secondary antibody followed by the addition of avidin-biotin-peroxidase complex, gives a superior result when compared to the unlabeled antibody method. The availability of biotin-binding sites in the complex is created by the incubation of a relative excess of avidin with biotin-labeled peroxidase. During formation of the complex, avidin acts as a bridge between biotin-labeled peroxidase molecules; and biotin-labeled peroxidase molecules, which contains several biotin moieties, serve as a link between the avidin molecules. Consequently, a "lattice" complex containing several peroxidase molecules is likely formed. Binding of this complex to the biotin moieties associated with secondary antibody results in a high staining intensity.
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(1992) · PubMed Central
Metadata
- Authors
- Hsu, S. M., Raine, L., & Fanger, H.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1981-04
- Volume
- 29
- Issue
- 4
- Pages
- 577-580
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2096428903
- Cited by
- 14,140
- References
- 0
- FWCI
- 51.43
- Type
- journal article
- Language
- en
- Open access
- bronze
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