Subpopulations of Mouse Blood Monocytes Differ in Maturation Stage and Inflammatory Response
Open accessAbstract
Blood monocytes are well-characterized precursors for macrophages and dendritic cells. Subsets of human monocytes with differential representation in various disease states are well known. In contrast, mouse monocyte subsets have been characterized minimally. In this study we identify three subpopulations of mouse monocytes that can be distinguished by differential expression of Ly-6C, CD43, CD11c, MBR, and CD62L. The subsets share the characteristics of extensive phagocytosis, similar expression of M-CSF receptor (CD115), and development into macrophages upon M-CSF stimulation. By eliminating blood monocytes with dichloromethylene-bisphosphonate-loaded liposomes and monitoring their repopulation, we showed a developmental relationship between the subsets. Monocytes were maximally depleted 18 h after liposome application and subsequently reappeared in the circulation. These cells were exclusively of the Ly-6Chigh subset, resembling bone marrow monocytes. Serial flow cytometric analyses of newly released Ly-6Chigh monocytes showed that Ly-6C expression on these cells was down-regulated while in circulation. Under inflammatory conditions elicited either by acute infection with Listeria monocytogenes or chronic infection with Leishmania major, there was a significant increase in immature Ly-6Chigh monocytes, resembling the inflammatory left shift of granulocytes. In addition, acute peritoneal inflammation recruited preferentially Ly-6Cmed-high monocytes. Taken together, these data identify distinct subpopulations of mouse blood monocytes that differ in maturation stage and capacity to become recruited to inflammatory sites.
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Macrophages have dynamic and plastic phenotypes that change with local microenvironmental signals, unlike lymphocytes whose phenotype is relatively fixed upon differentiation.
“It has been shown that unlike lymphocytes, where cellular phenotype is relatively fixed upon differentiation, macrophages have dynamic and plastic phenotypes that change with local microenvironmental signals [5,14–16].”
From Macrophage polarization: an opportunity for improved outcomes in biomaterials and regenerative medicine (2012) · cites this paper as [5]
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- Authors
- Sunderkötter, C., Nikolic, T., Dillon, M. J., van Rooijen, N., Stehling, M., Drevets, D. A., & Leenen, P. J. M.
- Affiliations
- Institute of Experimental Dermatology and Department of Dermatology, University of Münster , Münster ,
- Department of Immunology, Erasmus MC , Rotterdam ,
- Oklahoma City VA Medical Center (US)
- Department of Molecular Cell Biology, Free University Medical Center , Amsterdam ,
- University of Münster (DE)
- Erasmus MC (NL)
- Corresponding author
- Stehling, Martin
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-04-01
- Volume
- 172
- Issue
- 7
- Pages
- 4410-4417
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1956258286
- Cited by
- 1,124
- References
- 0
- Citations by year
- 2026: 8 · 2025: 15 · 2024: 33 · 2023: 39 · 2022: 49 · 2021: 39 · 2020: 42 · 2019: 58 · 2018: 47 · 2017: 72 · 2016: 65 · 2015: 62 · 2014: 75 · 2013: 65 · 2012: 67
- FWCI
- 10.91
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- Funders
- U.S. Department of Veterans Affairs
- Deutsche Forschungsgemeinschaft
- Diabetes Fonds (96.606)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Keywords
- monocyte subsets, monocyte maturation, Ly6C, CD43, CD11c, MBR, L-selectin, M-CSF receptor, macrophage differentiation, bone marrow-derived monocytes, monocyte depletion, Listeria monocytogenes infection, peritonitis, phagocytosis, mice
- MeSH terms
- Animals, Antigens, Ly, Cell Differentiation, Cells, Cultured, Cytoplasmic Granules, Female, Flow Cytometry, Inflammation, Listeriosis, Mice, Inbred C57BL, Monocytes, Down-Regulation, Immunophenotyping, Macrophage-1 Antigen, Leishmaniasis, Cutaneous, CD11b Antigen, Mice
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