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Whey protein isolate attenuates strength decline after eccentrically-induced muscle damage in healthy individuals

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Cooke, Matthew B, Rybalka, Emma, Stathis, Christos G, Cribb, Paul J, Hayes, Alan·2010·Journal of the International Society of Sports Nutrition
DOI 10.1186/1550-2783-7-30PMC PMC2955583
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Abstract

BACKGROUND: We examined the effects of short-term consumption of whey protein isolate on muscle proteins and force recovery after eccentrically-induced muscle damage in healthy individuals. METHODS: Seventeen untrained male participants (23 ± 5 yr, 180 ± 6 cm, 80 ± 11 kg) were randomly separated into two supplement groups: i) whey protein isolate (WPH; n = 9); or ii) carbohydrate (CHO; n = 8). Participants consumed 1.5 g/kg.bw/day supplement (~30 g consumed immediately, and then once with breakfast, lunch, in the afternoon and after the evening meal) for a period of 14 days following a unilateral eccentric contraction-based resistance exercise session, consisting of 4 sets of 10 repetitions at 120% of maximum voluntary contraction on the leg press, leg extension and leg flexion exercise machine. Plasma creatine kinase and lactate dehydrogenase (LDH) levels were assessed as blood markers of muscle damage. Muscle strength was examined by voluntary isokinetic knee extension using a Cybex dynamometer. Data were analyzed using repeated measures ANOVA with an alpha of 0.05. RESULTS: Isometric knee extension strength was significantly higher following WPH supplementation 3 (P < 0.05) and 7 (P < 0.01) days into recovery from exercise-induced muscle damage compared to CHO supplementation. In addition, strong tendencies for higher isokinetic forces (extension and flexion) were observed during the recovery period following WPH supplementation, with knee extension strength being significantly greater (P < 0.05) after 7 days recovery. Plasma LDH levels tended to be lower (P = 0.06) in the WPH supplemented group during recovery. CONCLUSIONS: The major finding of this investigation was that whey protein isolate supplementation attenuated the impairment in isometric and isokinetic muscle forces during recovery from exercise-induced muscle injury.

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  • Blood concentrations of muscle damage markers tended to be lower when four ~30-g doses of a hydrolyzed whey protein isolate were ingested for two weeks following the damaging bout.

    “Additionally, blood concentrations of muscle damage markers tended to be lower when four ~30-g doses of a hydrolyzed whey protein isolate were ingested for two weeks following the damaging bout.”

    From International Society of Sports Nutrition Position Stand: protein and exercise (2017) · cites this paper as [209]

    The Methods (Dietary Supplementation; page not provided) describe roughly four 30-g servings per day for 14 days after the exercise bout. The Results (Plasma Enzyme Activity; page not provided) report a tendency toward lower LDH in the whey group (P = 0.064), but no group effect for CK. Thus the trend is supported for one measured muscle-damage marker, not for the markers collectively. (LLM, conf=0.87)

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  • Three and seven days after a damaging exercise bout, maximal strength levels were higher in the hydrolyzed whey protein group compared to carbohydrate supplementation.

    “Three and seven days after completing the damaging exercise bout, maximal strength levels were higher in the hydrolyzed whey protein group compared to carbohydrate supplementation.”

    From International Society of Sports Nutrition Position Stand: protein and exercise (2017) · cites this paper as [209]

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Cited by

Sorted by citations

Authors
Cooke, M. B., Rybalka, E., Stathis, C. G., Cribb, P. J., & Hayes, A.
Affiliations
  • The University of Queensland (AU)
  • Victoria University (AU)
Corresponding author
Hayes, Alan
ORCID
Venue
Journal of the International Society of Sports Nutrition
Publisher
Informa UK Limited
Published
2010-01-05
Volume
7
Issue
1
Pages
30-30
ISSN
1550-2783 (ISSN-L)
DOI
10.1186/1550-2783-7-30
PMC
PMC2955583
OpenAlex
W2162394269
Cited by
101
References
6
Citations by year
2026: 7 · 2024: 6 · 2023: 4 · 2022: 6 · 2021: 13 · 2020: 6 · 2019: 7 · 2018: 8 · 2017: 9 · 2016: 7 · 2015: 8 · 2014: 5 · 2013: 5 · 2012: 7
FWCI
1.96
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of the International Society of Sports Nutrition (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Victoria University Research Repository (Victoria University) (submittedVersion)
  • Minerva Access (University of Melbourne) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
whey protein isolate, exercise-induced muscle damage, muscle strength recovery, eccentric training, isokinetic knee extension, creatine kinase, lactate dehydrogenase, muscle protein synthesis, protein supplementation, carbohydrate supplementation, healthy young men

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