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Cited by 708Infant Nutrition and HealthGut microbiota and healthInfant Development and Preterm Care

Intestinal dysbiosis in preterm infants preceding necrotizing enterocolitis: a systematic review and meta-analysis

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Pammi, Mohan, Cope, Julia, Tarr, Phillip I., Warner, Barbara B., Morrow, Ardythe L., Mai, Volker, Gregory, Katherine E., Kroll, J. Simon +7 more·2017·Microbiome
DOI 10.1186/s40168-017-0248-8PMC PMC5343300
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Abstract

BACKGROUND: Necrotizing enterocolitis (NEC) is a catastrophic disease of preterm infants, and microbial dysbiosis has been implicated in its pathogenesis. Studies evaluating the microbiome in NEC and preterm infants lack power and have reported inconsistent results. METHODS AND RESULTS: Our objectives were to perform a systematic review and meta-analyses of stool microbiome profiles in preterm infants to discern and describe microbial dysbiosis prior to the onset of NEC and to explore heterogeneity among studies. We searched MEDLINE, PubMed, CINAHL, and conference abstracts from the proceedings of Pediatric Academic Societies and reference lists of relevant identified articles in April 2016. Studies comparing the intestinal microbiome in preterm infants who developed NEC to those of controls, using culture-independent molecular techniques and reported α and β-diversity metrics, and microbial profiles were included. In addition, 16S ribosomal ribonucleic acid (rRNA) sequence data with clinical meta-data were requested from the authors of included studies or searched in public data repositories. We reprocessed the 16S rRNA sequence data through a uniform analysis pipeline, which were then synthesized by meta-analysis. We included 14 studies in this review, and data from eight studies were available for quantitative synthesis (106 NEC cases, 278 controls, 2944 samples). The age of NEC onset was at a mean ± SD of 30.1 ± 2.4 weeks post-conception (n = 61). Fecal microbiome from preterm infants with NEC had increased relative abundances of Proteobacteria and decreased relative abundances of Firmicutes and Bacteroidetes prior to NEC onset. Alpha- or beta-diversity indices in preterm infants with NEC were not consistently different from controls, but we found differences in taxonomic profiles related to antibiotic exposure, formula feeding, and mode of delivery. Exploring heterogeneity revealed differences in microbial profiles by study and the target region of the 16S rRNA gene (V1-V3 or V3-V5). CONCLUSIONS: Microbial dysbiosis preceding NEC in preterm infants is characterized by increased relative abundances of Proteobacteria and decreased relative abundances of Firmicutes and Bacteroidetes. Microbiome optimization may provide a novel strategy for preventing NEC.

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  • Alterations in microbiota may contribute to disease progression in some conditions, including inflammatory bowel diseases.

    “It is very likely that this may contribute to disease progression in some conditions, including inflammatory bowel diseases 22,23 ,”

    From Human microbiome myths and misconceptions (2023) · cites this paper as [23]

    The Background and Discussion (no page numbers available) implicate intestinal dysbiosis in the development of necrotizing enterocolitis in preterm infants, supporting the cautious idea that microbiota alterations may contribute to disease. The paper does not address inflammatory bowel diseases or establish an effect on disease progression in those conditions; its findings concern NEC onset. (LLM, conf=0.85)

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  1. [1]Neu J Walker WA Necrotizing enterocolitis N Engl J Med 2011 364 255 264 10.1056/NEJMra1005408 21247316 PMC3628622 · DOI · Paper page
  2. [2]Lin PW Stoll BJ Necrotising enterocolitis Lancet 2006 368 1271 1283 10.1016/S0140-6736(06)69525-1 17027734 · DOI
  3. [3]Fanaroff AA Stoll BJ Wright LL Carlo WA Ehrenkranz RA Stark AR Bauer CR Donovan EF Korones SB Laptook AR Trends in neonatal morbidity and mortality for very low birthweight infants Am J Obstet Gynecol 2007 196 147 10.1016/j.ajog.2006.09.014 17306659 · DOI
  4. [4]Lemons JA Bauer CR Oh W Korones SB Papile LA Stoll BJ Verter J Temprosa M Wright LL Ehrenkranz RA Very low birth weight outcomes of the National Institute of Child health and human development neonatal research network, January 1995 through December 1996. NICHD Neonatal Research Network Pediatrics 2001 107 E1 10.1542/peds.107.1.e1 11134465 · DOI
  5. [5]Holman RC Stoll BJ Curns AT Yorita KL Steiner CA Schonberger LB Necrotising enterocolitis hospitalisations among neonates in the United States Paediatr Perinat Epidemiol 2006 20 498 506 10.1111/j.1365-3016.2006.00756.x 17052286 · DOI
  6. [6]Stoll BJ Hansen NI Adams-Chapman I Fanaroff AA Hintz SR Vohr B Higgins RD Neurodevelopmental and growth impairment among extremely low-birth-weight infants with neonatal infection JAMA 2004 292 2357 2365 10.1001/jama.292.19.2357 15547163 · DOI
  7. [7]Claud EC Walker WA Bacterial colonization, probiotics, and necrotizing enterocolitis J Clin Gastroenterol 2008 42 Suppl 2 S46 52 10.1097/MCG.0b013e31815a57a8 18520617 · DOI
  8. [8]Nanthakumar NN Fusunyan RD Sanderson I Walker WA Inflammation in the developing human intestine: a possible pathophysiologic contribution to necrotizing enterocolitis Proc Natl Acad Sci U S A 2000 97 6043 6048 10.1073/pnas.97.11.6043 10823949 PMC18555 · DOI
  9. [9]Nanthakumar N Meng D Goldstein AM Zhu W Lu L Uauy R Llanos A Claud EC Walker WA The mechanism of excessive intestinal inflammation in necrotizing enterocolitis: an immature innate immune response PLoS One 2011 6 e17776 10.1371/journal.pone.0017776 21445298 PMC3061868 · DOI
  10. [10]Carlisle EM Morowitz MJ The intestinal microbiome and necrotizing enterocolitis Curr Opin Pediatr 2013 25 382 387 10.1097/MOP.0b013e3283600e91 23657248 · DOI
  11. [11]Wang Y Hoenig JD Malin KJ Qamar S Petrof EO Sun J Antonopoulos DA Chang EB Claud EC 16S rRNA gene-based analysis of fecal microbiota from preterm infants with and without necrotizing enterocolitis ISME J 2009 3 944 954 10.1038/ismej.2009.37 19369970 PMC2713796 · DOI
  12. [12]Afrazi A Sodhi CP Richardson W Neal M Good M Siggers R Hackam DJ New insights into the pathogenesis and treatment of necrotizing enterocolitis: toll-like receptors and beyond Pediatr Res 2011 69 183 188 10.1203/PDR.0b013e3182093280 21135755 PMC3125129 · DOI
  13. [13]Musemeche CA Kosloske AM Bartow SA Umland ET Comparative effects of ischemia, bacteria, and substrate on the pathogenesis of intestinal necrosis J Pediatr Surg 1986 21 536 538 10.1016/S0022-3468(86)80228-7 3723307 · DOI
  14. [14]Cotten CM Taylor S Stoll B Goldberg RN Hansen NI Sanchez PJ Ambalavanan N Benjamin DK Jr Prolonged duration of initial empirical antibiotic treatment is associated with increased rates of necrotizing enterocolitis and death for extremely low birth weight infants Pediatrics 2009 123 58 66 10.1542/peds.2007-3423 19117861 PMC2760222 · DOI
  15. [15]Alexander VN Northrup V Bizzarro MJ Antibiotic exposure in the newborn intensive care unit and the risk of necrotizing enterocolitis J Pediatr 2011 159 392 397 10.1016/j.jpeds.2011.02.035 21489560 PMC3137655 · DOI
  16. [16]Morowitz MJ Poroyko V Caplan M Alverdy J Liu DC Redefining the role of intestinal microbes in the pathogenesis of necrotizing enterocolitis Pediatrics 2010 125 777 785 10.1542/peds.2009-3149 20308210 · DOI
  17. [17]Sodhi CP Neal MD Siggers R Sho S Ma C Branca MF Prindle T Jr Russo AM Afrazi A Good M Intestinal epithelial toll-like receptor 4 regulates goblet cell development and is required for necrotizing enterocolitis in mice Gastroenterology 2012 143 708 718 10.1053/j.gastro.2012.05.053 22796522 PMC3584415 · DOI
  18. [18]Lu P Sodhi CP Hackam DJ Toll-like receptor regulation of intestinal development and inflammation in the pathogenesis of necrotizing enterocolitis Pathophysiology 2014 21 81 93 10.1016/j.pathophys.2013.11.007 24365655 PMC4067244 · DOI
  19. [19]Gevers D Knight R Petrosino JF Huang K McGuire AL Birren BW Nelson KE White O Methe BA Huttenhower C The Human Microbiome Project: a community resource for the healthy human microbiome PLoS Biol 2012 10 e1001377 10.1371/journal.pbio.1001377 22904687 PMC3419203 · DOI · Paper page
  20. [20]Human Microbiome Project C Structure, function and diversity of the healthy human microbiome Nature 2012 486 207 214 10.1038/nature11234 22699609 PMC3564958 · DOI · Paper page
  21. [22]Bell MJ Ternberg JL Feigin RD Keating JP Marshall R Barton L Brotherton T Neonatal necrotizing enterocolitis, therapeutic decisions based upon clinical staging Ann Surg 1978 187 1 7 10.1097/00000658-197801000-00001 413500 PMC1396409 · DOI
  22. [25]Lozupone CA Stombaugh J Gonzalez A Ackermann G Wendel D Vazquez-Baeza Y Jansson JK Gordon JI Knight R Meta-analyses of studies of the human microbiota Genome Res 2013 23 1704 1714 10.1101/gr.151803.112 23861384 PMC3787266 · DOI · Paper page
  23. [26]Viswanathan M, Berkman ND, Dryden DM, Hartling L. Assessing Risk of Bias and Confounding in Observational Studies of Interventions or Exposures: Further Development of the RTI Item Bank. Methods Research Report. (Prepared by RTI–UNC Evidence-based Practice Center under Contract No. 290-2007-10056-I). AHRQ Publication No. 13-EHC106-EF. Rockville: Agency for Healthcare Research and Quality; 2013. www.effectivehealthcare.ahrq.gov/reports/final.cfm. Accessed 27 June 2015. 24006553
  24. [27]Caporaso JG Kuczynski J Stombaugh J Bittinger K Bushman FD Costello EK Fierer N Pena AG Goodrich JK Gordon JI QIIME allows analysis of high-throughput community sequencing data Nat Methods 2010 7 335 336 10.1038/nmeth.f.303 20383131 PMC3156573 · DOI · Paper page
  25. [28]McDonald D Price MN Goodrich J Nawrocki EP DeSantis TZ Probst A Andersen GL Knight R Hugenholtz P An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea ISME J 2012 6 610 618 10.1038/ismej.2011.139 22134646 PMC3280142 · DOI
  26. [29]Edgar RC Search and clustering orders of magnitude faster than BLAST Bioinformatics 2010 26 2460 2461 10.1093/bioinformatics/btq461 20709691 · DOI
  27. [30]Mshvildadze M Neu J Shuster J Theriaque D Li N Mai V Intestinal microbial ecology in premature infants assessed with non-culture-based techniques J Pediatr 2010 156 20 25 10.1016/j.jpeds.2009.06.063 19783002 PMC3628625 · DOI
  28. [31]Mai V Young CM Ukhanova M Wang X Sun Y Casella G Theriaque D Li N Sharma R Hudak M Neu J Fecal microbiota in premature infants prior to necrotizing enterocolitis PLoS One 2011 6 e20647 10.1371/journal.pone.0020647 21674011 PMC3108958 · DOI
  29. [32]McMurtry VE Gupta RW Tran L Blanchard EE Penn D Taylor CM Ferris MJ Bacterial diversity and Clostridia abundance decrease with increasing severity of necrotizing enterocolitis Microbiome 2015 3 11 10.1186/s40168-015-0075-8 25810906 PMC4373520 · DOI
  30. [33]Morrow AL Lagomarcino AJ Schibler KR Taft DH Yu Z Wang B Altaye M Wagner M Gevers D Ward DV Early microbial and metabolomic signatures predict later onset of necrotizing enterocolitis in preterm infants Microbiome 2013 1 13 10.1186/2049-2618-1-13 24450576 PMC3971624 · DOI
  31. [34]Normann E Fahlen A Engstrand L Lilja HE Intestinal microbial profiles in extremely preterm infants with and without necrotizing enterocolitis Acta Paediatr 2013 102 129 136 10.1111/apa.12059 23082780 · DOI
  32. [36]Torrazza RM Ukhanova M Wang X Sharma R Hudak ML Neu J Mai V Intestinal microbial ecology and environmental factors affecting necrotizing enterocolitis PLoS One 2013 8 e83304 10.1371/journal.pone.0083304 24386174 PMC3875440 · DOI
  33. [37]Zhou Y Shan G Sodergren E Weinstock G Walker WA Gregory KE Longitudinal analysis of the premature infant intestinal microbiome prior to necrotizing enterocolitis: a case-control study PLoS One 2015 10 e0118632 10.1371/journal.pone.0118632 25741698 PMC4351051 · DOI
  34. [38]Warner BB, Deych E, Zhou Y, Hall-Moore C, Weinstock GM, Sodergren E, Shaikh N, Hoffmann JA, Linneman LA, Hamvas A, et al. Gut bacteria dysbiosis and necrotising enterocolitis in very low birthweight infants: a prospective case–control study. Lancet. 2016;387(10031):1928–36. PMC5553277 26969089
  35. [39]de la Cochetiere MF Piloquet H des Robert C Darmaun D Galmiche JP Roze JC Early intestinal bacterial colonization and necrotizing enterocolitis in premature infants: the putative role of Clostridium Pediatr Res 2004 56 366 370 10.1203/01.PDR.0000134251.45878.D5 15201403 · DOI
  36. [40]Stewart CJ Marrs EC Magorrian S Nelson A Lanyon C Perry JD Embleton ND Cummings SP Berrington JE The preterm gut microbiota: changes associated with necrotizing enterocolitis and infection Acta Paediatr 2012 101 1121 1127 10.1111/j.1651-2227.2012.02801.x 22845166 · DOI
  37. [41]Yee WH Soraisham AS Shah VS Aziz K Yoon W Lee SK Incidence and timing of presentation of necrotizing enterocolitis in preterm infants Pediatrics 2012 129 e298 304 10.1542/peds.2011-2022 22271701 · DOI
  38. [42]Gonzalez-Rivera R Culverhouse RC Hamvas A Tarr PI Warner BB The age of necrotizing enterocolitis onset: an application of Sartwell’s incubation period model J Perinatol 2011 31 519 523 10.1038/jp.2010.193 21273988 PMC3145821 · DOI
  39. [43]Llanos AR Moss ME Pinzon MC Dye T Sinkin RA Kendig JW Epidemiology of neonatal necrotising enterocolitis: a population-based study Paediatr Perinat Epidemiol 2002 16 342 349 10.1046/j.1365-3016.2002.00445.x 12445151 · DOI
  40. [44]La Rosa PS Warner BB Zhou Y Weinstock GM Sodergren E Hall-Moore CM Stevens HJ Bennett WE Jr Shaikh N Linneman LA Patterned progression of bacterial populations in the premature infant gut Proc Natl Acad Sci U S A 2014 111 12522 12527 10.1073/pnas.1409497111 25114261 PMC4151715 · DOI
  41. [45]Sodhi C Richardson W Gribar S Hackam DJ The development of animal models for the study of necrotizing enterocolitis Dis Model Mech 2008 1 94 98 10.1242/dmm.000315 19048070 PMC2562191 · DOI
  42. [46]Sodhi CP Shi XH Richardson WM Grant ZS Shapiro RA Prindle T Jr Branca M Russo A Gribar SC Ma C Hackam DJ Toll-like receptor-4 inhibits enterocyte proliferation via impaired beta-catenin signaling in necrotizing enterocolitis Gastroenterology 2010 138 185 196 10.1053/j.gastro.2009.09.045 19786028 PMC2813409 · DOI
  43. [47]Jilling T Simon D Lu J Meng FJ Li D Schy R Thomson RB Soliman A Arditi M Caplan MS The roles of bacteria and TLR4 in rat and murine models of necrotizing enterocolitis J Immunol 2006 177 3273 3282 10.4049/jimmunol.177.5.3273 16920968 PMC2697969 · DOI
  44. [48]Ward DV Scholz M Zolfo M Taft DH Schibler KR Tett A Segata N Morrow AL Metagenomic sequencing with strain-level resolution implicates uropathogenic E. coli in necrotizing enterocolitis and mortality in preterm infants Cell Rep 2016 14 2912 2924 10.1016/j.celrep.2016.03.015 26997279 PMC4819403 · DOI
  45. [49]Morowitz MJ Denef VJ Costello EK Thomas BC Poroyko V Relman DA Banfield JF Strain-resolved community genomic analysis of gut microbial colonization in a premature infant Proc Natl Acad Sci U S A 2011 108 1128 1133 10.1073/pnas.1010992108 21191099 PMC3024690 · DOI
  46. [50]Zeissig S Blumberg RS Life at the beginning: perturbation of the microbiota by antibiotics in early life and its role in health and disease Nat Immunol 2014 15 307 310 10.1038/ni.2847 24646587 · DOI
  47. [51]Gibson MK Wang B Ahmadi S Burnham CA Tarr PI Warner BB Dantas G Developmental dynamics of the preterm infant gut microbiota and antibiotic resistome Nat Microbiol 2016 1 16024 10.1038/nmicrobiol.2016.24 27572443 PMC5031140 · DOI · Paper page
  48. [52]Barb JJ Oler AJ Kim HS Chalmers N Wallen GR Cashion A Munson PJ Ames NJ Development of an analysis pipeline characterizing multiple hypervariable regions of 16S rRNA using mock samples PLoS One 2016 11 e0148047 10.1371/journal.pone.0148047 26829716 PMC4734828 · DOI
  49. [53]Zheng W Tsompana M Ruscitto A Sharma A Genco R Sun Y Buck MJ An accurate and efficient experimental approach for characterization of the complex oral microbiota Microbiome 2015 3 48 10.1186/s40168-015-0110-9 26437933 PMC4593206 · DOI
  50. [54]Kumar PS Brooker MR Dowd SE Camerlengo T Target region selection is a critical determinant of community fingerprints generated by 16S pyrosequencing PLoS One 2011 6 e20956 10.1371/journal.pone.0020956 21738596 PMC3126800 · DOI
  51. [55]Taft DH Ambalavanan N Schibler KR Yu Z Newburg DS Ward DV Morrow AL Intestinal microbiota of preterm infants differ over time and between hospitals Microbiome 2014 2 36 10.1186/2049-2618-2-36 25332767 PMC4203463 · DOI
  52. [56]Torrazza RM Neu J The altered gut microbiome and necrotizing enterocolitis Clin Perinatol 2013 40 93 108 10.1016/j.clp.2012.12.009 23415266 PMC3589666 · DOI
  53. [57]Schwiertz A Gruhl B Lobnitz M Michel P Radke M Blaut M Development of the intestinal bacterial composition in hospitalized preterm infants in comparison with breast-fed, full-term infants Pediatr Res 2003 54 393 399 10.1203/01.PDR.0000078274.74607.7A 12788986 · DOI
  54. [58]Bjorkstrom MV Hall L Soderlund S Hakansson EG Hakansson S Domellof M Intestinal flora in very low-birth weight infants Acta Paediatr 2009 98 1762 1767 10.1111/j.1651-2227.2009.01471.x 19673724 · DOI
  55. [59]Latuga MS Stuebe A Seed PC A review of the source and function of microbiota in breast milk Semin Reprod Med 2014 32 68 73 10.1055/s-0033-1361824 24390923 · DOI
  56. [60]Sharon I Morowitz MJ Thomas BC Costello EK Relman DA Banfield JF Time series community genomics analysis reveals rapid shifts in bacterial species, strains, and phage during infant gut colonization Genome Res 2013 23 111 120 10.1101/gr.142315.112 22936250 PMC3530670 · DOI
  57. [61]Grishin A Papillon S Bell B Wang J Ford HR The role of the intestinal microbiota in the pathogenesis of necrotizing enterocolitis Semin Pediatr Surg 2013 22 69 75 10.1053/j.sempedsurg.2013.01.002 23611609 PMC3647029 · DOI
  58. [62]Raveh-Sadka T, Thomas BC, Singh A, Firek B, Brooks B, Castelle CJ, Sharon I, Baker R, Good M, Morowitz MJ, Banfield JF. Gut bacteria are rarely shared by co-hospitalized premature infants, regardless of necrotizing enterocolitis development. Elife. 2015; 4. PMC4384745 25735037
  59. [63]Millar MR, Linton CJ, Cade A, Glancy D, Hall M, Jalal H. Application of 16S rRNA gene PCR to study bowel flora of preterm infants with and without necrotizing enterocolitis. J Clin Microbiol. 1996;34(10):2506–10.​ PMC229306 8880510
  60. [64]Smith B, Bodé S, Skov TH, Mirsepasi H, Greisen G, Krogfelt KA. Investigation of the early intestinal microflora in premature infants with/without necrotizing enterocolitis using two different methods. Pediatr Res. 2012;71(1):115–20. 22289859

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Authors
Pammi, M., Cope, J., Tarr, P. I., Warner, B. B., Morrow, A. L., Mai, V., Gregory, K. E., Kroll, J. S., McMurtry, V., Ferris, M. J., Engstrand, L., Lilja, H. E., Hollister, E. B., Versalovic, J., & Neu, J.
Affiliations
  • Baylor College of Medicine (US)
  • Washington University in St. Louis (US)
  • Cincinnati Children's Hospital Medical Center (US)
  • University of Florida Health (US)
  • Brigham and Women's Hospital (US)
  • Imperial College London (GB)
  • Children's Hospital of New Orleans (US)
  • Karolinska Institutet (SE)
Corresponding author
Pammi, Mohan
ORCID
Venue
Microbiome
Publisher
Springer Science and Business Media LLC
Published
2017-03-09
Volume
5
Issue
1
Pages
31-31
ISSN
2049-2618 (ISSN-L)
DOI
10.1186/s40168-017-0248-8
PMC
PMC5343300
OpenAlex
W2592537218
Cited by
708
References
4
Citations by year
2026: 68 · 2025: 76 · 2024: 83 · 2023: 107 · 2022: 87 · 2021: 92 · 2020: 64 · 2019: 55 · 2018: 51 · 2017: 24
FWCI
5.46
Type
journal article
Language
en
Open access
Licence
Locations
  • Microbiome (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Uppsala University Publications (Uppsala University) (submittedVersion, other-oa)
  • Spiral (Imperial College London) (submittedVersion, other-oa)
Funders
  • National Institute of Diabetes and Digestive and Kidney Diseases (P30DK052574)
  • National Institute of Allergy and Infectious Diseases (UH3 AI083265)
  • Washington University Institute of Clinical and Translational Science (UL1RR024992)
  • National Institutes of Health (K23 NR011320-01)
  • Harvard Catalyst CTSA Grant (UL1 RR 025758-01)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01 HD059140)
  • National Institute for Health Research (NIHR) Biomedical Research Centre
  • Winnicott Foundation
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Infant Development and Preterm Care
Keywords
necrotizing enterocolitis, preterm infants, gut dysbiosis, gut microbiota, fecal microbiota, Proteobacteria, Firmicutes, Bacteroidetes, microbial diversity, antibiotic exposure, bottle feeding, mode of delivery, NEC prevention
MeSH terms
Firmicutes, Bacillota, Gastrointestinal Microbiome, Bacteria, Bacteroides, Feces, Humans, Infant, Newborn, Infant, Premature, Infant, Premature, Diseases, Intestines, RNA, Ribosomal, 16S, Enterocolitis, Necrotizing, Proteobacteria, Dysbiosis

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