Androgenic activity of dehydroepiandrosterone and androstenedione in the rat ventral prostate.
Abstract
In order to assess the androgenic potency of physiological plasma concentrations of the adrenal steroids dehydroepiandrosterone (DHEA) and androstenedione (delta 4-dione) in the rat prostate, these two steroids were released from Silastic tubings of appropriate length and size in castrated male rats. Implants of DHEA led to plasma levels of DHEA and 5-androsten-3 beta,17 beta-diol covering the range of concentrations found in adult men while no significant change was observed in plasma levels of delta 4-dione, testosterone and dihydrotestosterone (DHT). delta 4-Dione implants, on the other hand, led to a parallel increase in plasma delta 4-dione and testosterone levels at all doses used while plasma DHT only increased at supraphysiological doses of delta 4-dione. At plasma concentrations comparable to those found in adult men; delta 4-dione (0.8 ng/ml) and DHEA (3.4 ng/ml) stimulated prostate weight 3.7- and 2.1-fold, respectively. In the same groups, prostatic DHT levels were elevated at 4.48 +/- 0.05 and 2.70 +/- 0.73 ng/g tissue, respectively. A close parallelism was observed between prostatic DHT levels and prostatic weight in all groups. The present data show that in the rat, a species having no significant secretion of adrenal androgens, plasma concentrations of DHEA and delta 4-dione maintained within the range of those found in adult men are efficiently converted into DHT and act as potent androgenic stimuli in prostatic tissue. The castrated rat bearing Silastic implants releasing constant and predetermined amounts of adrenal steroids offers a good model to study the recently identified role of adrenal steroids in peripheral tissues.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Most of the androgens in women, especially after menopause, are synthesized in peripheral intracrine tissues from the inactive precursors DHEA and DHEA-S of adrenal origin.
“most of the androgens in women, especially after menopause, are synthesized in peripheral intracrine tissues from the inactive precursors dehydroepiandrosterone (DHEA) and DHEA sulfate (DHEA-S) of adrenal origin.”
From Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone (2003) · cites this paper as [78]
Man is unique, with some other primates, in having adrenals that secrete large amounts of the precursor steroids DHEA and DHEA-S, which are converted into 4-dione and then into potent androgens and/or estrogens in peripheral tissues.
“Man is unique, with some other primates, in having adrenals that secrete large amounts of the precursor steroids DHEA and DHEA-S, which are converted into 4-dione and then into potent androgens and/or estrogens in peripheral tissues (Refs.25, 77, and 78; Fig. 3).”
From Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone (2003) · cites this paper as [78]
DHEA possesses relatively potent androgenic activity and stimulates androgen-dependent gene expression in the rat ventral prostate.
“Labrie and colleagues (78, 249) first demonstrated that DHEA possesses relatively potent androgenic activity and stimulates androgen-dependent gene expression in the rat ventral prostate.”
From Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone (2003) · cites this paper as [78]
Human adrenals secrete large amounts of precursor steroids DHEA and DHEA-S, both converted into androgens in target intracrine tissues.
“the human adrenals secrete large amounts of the precursor steroids DHEA and DHEA-S, both of which are converted into androgens in target intracrine tissues (25,35,78,92,249,254,255).”
From Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone (2003) · cites this paper as [78]
DHEA is converted into androgens, and treatment with DHEA induces androgen-sensitive gene expression in the rat ventral prostate.
“DHEA is well known to be converted into androgens, and treatment with DHEA is known to induce androgen-sensitive gene expression in the rat ventral prostate (78, 249).”
From Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone (2003) · cites this paper as [78]
Inhibition of tumor growth seen after DHEA treatment in ovariectomized animals results from its intracrine in situ conversion into androgens in the mammary gland.
“the inhibition of tumor growth seen after DHEA treatment in ovariectomized animals results from its intracrine in situ conversion into androgens in the mammary gland (25,35,78,92,255).”
From Endocrine and intracrine sources of androgens in women: inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone (2003) · cites this paper as [78]
Cites
Extension of Multiple Range Tests to Group Means with Unequal Numbers of Replications
Kramer, C. Y. (1956) · Biometrics · doi:10.2307/3001469 · Cited by 3,146 · see citing works
COMPUTER ANALYSIS OF RADIOLIGAND ASSAY AND RADIOIMMUNOASSAY DATA
Rodbard, D., & Lewald, J. E. (1970) · European Journal of Endocrinology · doi:10.1530/acta.0.065s079 · Cited by 608 · see citing works
Complete androgen blockade for the treatment of prostate cancer.
Labrie, F., Dupont, A., & Bèlanger, A. (1985) · PubMed · Cited by 285 · see citing works
Self-citationSelective Effect of Androgens on LH and FSH Release in Anterior Pituitary Cells in Culture
Drouin, J., & Labrie, F. (1976) · Endocrinology · doi:10.1210/endo-98-6-1528 · Cited by 255 · see citing works
Self-citationSTEROIDS OF ADRENAL ORIGIN METABOLIZED BY HUMAN PROSTATIC TISSUE BOTH IN VIVO AND IN VITRO
Harper, M. E., Pike, A. W., Peeling, W. B., & Griffiths, K. (1974) · Journal of Endocrinology · doi:10.1677/joe.0.0600117 · Cited by 190 · see citing works
Simultaneous radioimmunoassay of progestins, androgens and estrogens in rat testis
Bèlanger, A., Caron, S., & Picard, V. (1980) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(80)90190-9 · Cited by 177 · see citing works
STUDIES ON THE SECRETION AND INTERCONVERSION OF THE ANDROGENS.
VandeWiele, R. L., Pc, M., Gurpide, E., & Lieberman, S. (1963) · PubMed · Cited by 144 · see citing works
Levels of plasma steroid glucuronides in intact and castrated men with prostatic cancer.
BÉLANGER, A., BROCHU, M., & CLICHE, J. (1986) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-62-5-812 · Cited by 108 · see citing works
ADRENAL SECRETION OF ANDROGENS AND OESTROGENS
Baird, D. T., Uno, A., & Melby, J. C. (1969) · Journal of Endocrinology · doi:10.1677/joe.0.0450135 · Cited by 108 · see citing works
Origin of Plasma Androstanediol Glucuronide in Men*
Moghissi, E. S., Ablan, F., & Horton, R. (1984) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-59-3-417 · Cited by 102 · see citing works
Kirschner, M. A., SINHAMAHAPATRA, S. B., Zucker, I., LORIAUX, L. D., & Nieschlag, E. (1973) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-37-2-183 · Cited by 96 · see citing works
The Effects of Castration on Adrenal Testosterone Secretion in Men with Prostatic Carcinoma
Sanford, E. J., Paulson, D. F., Rohner, T. J., Drago, J. R., Santen, R. J., & Bardin, C. W. (1977) · The Journal of Urology · doi:10.1016/s0022-5347(17)58283-x · Cited by 90 · see citing works
Metabolism of free and sulfoconjugated DHEA in brain tissue in vivo and in vitro
Knapstein, P., David, A., Wu, C.-H., Archer, D. F., Flickinger, G. L., & Touchstone, J. C. (1968) · Steroids · doi:10.1016/s0039-128x(68)80102-3 · Cited by 84 · see citing works
Intratissular androgens in benign prostatic hyperplasia and prostatic cancer
Voigt, K., & BARTSCH, W. (1986) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(86)90304-3 · Cited by 71 · see citing works
Bélanger, A., Dupont, A., & Labrie, F. (1984) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-59-3-422 · Cited by 70 · see citing works
Self-citationTransformation and Conjugation of Dehydroepiandrosterone by Human Skin1
Gallegos, A. J., & Berliner, D. L. (1967) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-27-8-1214 · Cited by 66 · see citing works
Moguilewsky, M., Fiet, J., Tournemine, C., & Raynaud, J. (1986) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(86)90043-9 · Cited by 66 · see citing works
Androgen Metabolism in Human Skin
Flamigni, C., Collins, W. P., KOULLAPIS, E. N., Craft, I., Dewhurst, C., & Sommerville, I. F. (1971) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-32-6-737 · Cited by 63 · see citing works
Guignard, M., Pesquiès, P., Serrurier, B., Gomez-Mérino, D., & Reinberg, A. (1980) · European Journal of Endocrinology · doi:10.1530/acta.0.0940536 · Cited by 58 · see citing works
van der Molen, H. J., & Groen, D. (1968) · European Journal of Endocrinology · doi:10.1530/acta.0.0580419 · Cited by 48 · see citing works
Plasma levels of steroid glucuronides in prepubertal, adult and elderly men
Bélanger, A., Brochu, M., & Clich, J. (1986) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(86)90361-4 · Cited by 41 · see citing works
Testosterone metabolism in vivo by human prostatic tissue
Peeling, W. B., Pike, A. W., Harper, M. E., PIERREPOINT, C. G., & Griffiths, K. (1970) · Biochemical Journal · doi:10.1042/bj1200443 · Cited by 39 · see citing works
Role of Sex Steroids on LH and FSH Secretion in the Rat
Ferland, L., Drouin, J., & Labrie, F. (1976) · Springer eBooks · doi:10.1007/978-1-4684-2598-7_12 · Cited by 36 · see citing works
Self-citationIN VITRO METABOLISM OF ANDROGENS IN WHOLE HUMAN BLOOD
Blaquier, J. A., Forchielli, E., & Dorfman, R. I. (1967) · European Journal of Endocrinology · doi:10.1530/acta.0.0550697 · Cited by 31 · see citing works
LHRH Agonists and Antiandrogens in Prostate Cancer
Labrie, F., Dupont, A., & Bélanger, A. (1987) · Cancer treatment and research · doi:10.1007/978-1-4613-2033-3_15 · Cited by 29 · see citing works
Self-citationCowley, T., Brownsey, B. G., Harper, M. E., Peeling, W. B., & Griffiths, K. (1976) · European Journal of Endocrinology · doi:10.1530/acta.0.0810310 · Cited by 29 · see citing works
Acevedo, H. F., & Goldzieher, J. W. (1965) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(65)90170-4 · Cited by 26 · see citing works
Androgen metabolism in human skin.
Sommerville, I. F., Collins, W. P., Flamigni, C., KOULLAPIS, E. N., & Dewhurst, C. J. (1971) · Europe PMC (PubMed Central) · Cited by 9 · see citing works
METABOLISM OF DEHYDROEPIANDROSTERONE BY ISOLATED HUMAN HAIR FOLLICLES
Fazekas, A. G. (1971) · European Journal of Endocrinology · doi:10.1530/acta.0.067s119 · Cited by 7 · see citing works
Nishida, S., Matsumura, S., Horino, M., et al. (1977) · Steroids · doi:10.1016/s0039-128x(77)80022-6 · Cited by 5 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Labrie, F., Bélanger, A., Cusan, L., Gomez, J.-L., & Candas, B. (1997) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.82.8.4160 · Cited by 713 · see citing works
Self-citationLabrie, F. (1991) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(91)90116-a · Cited by 685 · see citing works
Self-citationLabrie, F., Luu-The, V., Labrie, C., Bélanger, A., Simard, J., Lin, S.-X., & Pelletier, G. (2003) · Endocrine Reviews · doi:10.1210/er.2001-0031 · Cited by 544 · see citing works
Self-citationThe key role of 17β-hydroxysteroid dehydrogenases in sex steroid biology
Labrie, F., Luu-The, V., Lin, S.-X., Claude, L., Simard, J., Breton, R., & Bélanger, A. (1997) · Steroids · doi:10.1016/s0039-128x(96)00174-2 · Cited by 480 · see citing works
Self-citationLabrie, F., Luu-The, V., Labrie, C., & Simard, J. (2001) · Frontiers in Neuroendocrinology · doi:10.1006/frne.2001.0216 · Cited by 366 · see citing works
Self-citationRhéaume, E., Lachance, Y., Zhao, H.-F., Breton, N., Dumont, M., Launoit, Y. D., Trudel, C., Luu-The, V., Simard, J., & Labrie, F. (1991) · Molecular Endocrinology · doi:10.1210/mend-5-8-1147 · Cited by 362 · see citing works
Self-citationLabrie, F., Bélanger, A., Cusan, L., & Candas, B. (1997) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.82.8.4161 · Cited by 350 · see citing works
Self-citationLabrie, F., Bélanger, A., Luu-The, V., Labrie, C., Simard, J., Cusan, L., Gomez, J.-L., & Candas, B. (1998) · Steroids · doi:10.1016/s0039-128x(98)00007-5 · Cited by 347 · see citing works
Self-citationLabrie, F., Luu-The, V, Lin, S., Simard, J., Labrie, C., El-Alfy, M., Pelletier, G., & Belanger, A. (2000) · Journal of Molecular Endocrinology · doi:10.1677/jme.0.0250001 · Cited by 278 · see citing works
Self-citationDHEA and peripheral androgen and estrogen formation: intracrinology
LABRIE, F., BÉLANGER, A., SIMARD, J., LUU‐THE, V., & LABRIE, C. (1995) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1995.tb17369.x · Cited by 280 · see citing works
Self-citationLabrie, F., Diamond, P., Cusan, L., Gomez, J.-L., Bélanger, A., & Candas, B. (1997) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.82.10.4306 · Cited by 292 · see citing works
Self-citationLabrie, F. (2015) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2014.06.001 · Cited by 227 · see citing works
Self-citationIntracrine androgen biosynthesis, metabolism and action revisited
Schiffer, L., Arlt, W., & Storbeck, K.-H. (2018) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2017.08.016 · Cited by 217 · see citing works
“…enzymatically inactivated in the same cell prior to efflux; (6) no significant amount of active hormones are released from the cell into the extracellular space (s paracrinology) or circulation (s endocrinology) to prevent a systemic excess of active hormones (Labrie, 1991; Labrie et al., 1997a).”
InfluentialMarchais-Oberwinkler, S., Henn, C., Möller, G., Klein, T., Negri, M., Oster, A., Spadaro, A., Werth, R., Wetzel, M., Xu, K., et al. (2011) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2010.12.013 · Cited by 216 · see citing works
Reduced testosterone and adrenal C19 steroid levels in obese men
Tchernof, A., Després, J.-P., Bélanger, A., Dupont, A., Prud'homme, D., Moorjani, S., Lupien, P. J., & Labrie, F. (1995) · Metabolism · doi:10.1016/0026-0495(95)90060-8 · Cited by 175 · see citing works
Self-citationA new dawn for androgens: novel lessons from 11-oxygenated C19 steroids
Pretorius, E., Arlt, W., & Storbeck, K.-H. (2017) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2016.08.014 · Cited by 142 · see citing works
Labrie, F., Martel, C., & Balser, J. (2011) · Menopause · doi:10.1097/gme.0b013e3181e195a6 · Cited by 143 · see citing works
Labrie, F., Simard, J., Luu-The, V., Bélanger, A., & Pelletier, G. (1992) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(92)90308-6 · Cited by 132 · see citing works
Self-citationMetabolism of DHEA in postmenopausal women following percutaneous administration
Labrie, F., Bélanger, A., Bélanger, P., Bérubé, R., Martel, C., Cusan, L., Gomez, J., Candas, B., Chaussade, V., Castiel, I., et al. (2007) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2006.09.034 · Cited by 129 · see citing works
Self-citationSwart, A. C., Schloms, L., Storbeck, K.-H., Bloem, L. M., Toit, T. D., Quanson, J. L., Rainey, W. E., & Swart, P. (2013) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2013.04.010 · Cited by 125 · see citing works
Androgens and endometrium: new insights and new targets
Simitsidellis, I., Saunders, P. T. K., & Gibson, D. A. (2018) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2017.09.022 · Cited by 107 · see citing works
Labrie, F., Simard, J., Luu-The, V., Pelletier, G., Bélanger, A., Lachance, Y., Zhao, H. F., Labrie, C., Breton, N., de Launoit, Y., et al. (1992) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(92)90368-s · Cited by 88 · see citing works
LABRIE, C., SIMARD, J., ZHAO, H. F., BELANGER, A., PELLETIER, G., & LABRIE, F. (1989) · Endocrinology · doi:10.1210/endo-124-6-2745 · Cited by 80 · see citing works
Self-citationVandenput, L., Labrie, F., Mellström, D., Swanson, C., Knutsson, T., Peeker, R., Ljunggren, Ö., Orwoll, E., Eriksson, A. L., Damber, J.-E., et al. (2007) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.061018 · Cited by 71 · see citing works
Self-citationLi, S., Yan, X., B�langer, A., & Labrie, F. (1994) · Breast Cancer Research and Treatment · doi:10.1007/bf00665681 · Cited by 70 · see citing works
Self-citationAlmost exclusive androgenic action of dehydroepiandrosterone in the rat mammary gland.
Sourla, A., Martel, C., Labrie, C., & Labrie, F. (1998) · Endocrinology · doi:10.1210/endo.139.2.5762 · Cited by 49 · see citing works
Self-citationInhibitory effect of androgens on DMBA-induced mammary carcinoma in the rat.
Dauvois, S., Li, S., Martel, C., & Labrie, F. (1989) · Breast Cancer Research and Treatment · doi:10.1007/bf01806301 · Cited by 47 · see citing works
Self-citationEffect of dehydroepiandrosterone on vaginal and uterine histomorphology in the rat.
Sourla, A., Flamand, M., Bélanger, A., & Labrie, F. (1998) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/s0960-0760(98)00052-1 · Cited by 40 · see citing works
Self-citationCouillard, S., Labrie, C., Bélanger, A., Candas, B., Pouliot, F., & Labrie, F. (1998) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/90.10.772 · Cited by 38 · see citing works
Self-citationLuo, S., Labrie, C., Bélanger, A., & Labrie, F. (1997) · Endocrinology · doi:10.1210/endo.138.8.5345 · Cited by 34 · see citing works
Self-citationLabrie, F., & Martel, C. (2017) · Hormone Molecular Biology and Clinical Investigation · doi:10.1515/hmbci-2016-0042 · Cited by 17 · see citing works
Self-citation
- Authors
- LABRIE, C., BELANGER, A., & LABRIE, F.
- Affiliations
- Université Laval (CA)
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1988-09
- Volume
- 123
- Issue
- 3
- Pages
- 1412-1417
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2022453124
- Cited by
- 167
- References
- 30
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 4 · 2014: 7 · 2013: 3 · 2012: 3
- FWCI
- 5.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Prostate Cancer Treatment and Research
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- dehydroepiandrosterone, androstenedione, androgenic activity, ventral prostate, dihydrotestosterone, adrenal steroids, steroid implants, castration, prostate enlargement, testosterone, rat model
- MeSH terms
- Androgens, Androstenedione, Animals, Dehydroepiandrosterone, Kinetics, Male, Orchiectomy, Prostate, Rats, Inbred Strains, Reference Values, Silicone Elastomers, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
