The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Hypertrophy and Increased Gene Expression of Neurons Containing Neurokinin-B and Substance-P Messenger Ribonucleic Acids in the Hypothalami of Postmenopausal Women*
Abstract
We have previously described hypertrophy of neurons containing estrogen receptor mRNA in the infundibular nucleus of postmenopausal women. In the present investigation we identified peptide mRNAs in the hypertrophied neurons and determined whether postmenopausal neuronal hypertrophy was accompanied by changes in gene expression. In the first study in situ hybridization was performed on sections from hypothalami of postmenopausal women (n = 3) using synthetic 35S-labeled cDNA probes complementary to mRNAs encoding estrogen receptor, substance-P (SP), neurokinin-B (NKB), POMC, cholecystokinin, dynorphin, CRF, enkephalin, galanin, neuropeptide-Y, GH-releasing hormone, and tyrosine hydroxylase. Neuronal cross-sectional areas and cell densities were measured with the aid of a computer microscope system. Neurons labeled with the NKB and SP probes were comparable in size, morphology, and distribution to the hypertrophied neurons containing estrogen receptor mRNA. In contrast, neurons labeled with other cDNA probes were sparsely distributed (CRF and dynorphin), smaller in size (neuropeptide-Y, galanin, GH-releasing hormone, enkephalin, cholecystokinin, and POMC), or located anterior to the hypertrophied population (tyrosine hydroxylase). In the second study sections from hypothalami of premenopausal (n = 3) and postmenopausal (n = 3) women were incubated with cDNA probes complementary to SP or NKB mRNAs. The mean cross-sectional areas of postmenopausal infundibular neurons containing NKB and SP mRNAs increased to 194% and 176% of premenopausal values, respectively. The autoradiographic grain densities of infundibular neurons labeled with either probe were also significantly increased in the postmenopausal group. Finally, the numbers of labeled neurons/tissue increased 6-fold (SP) and 15-fold (NKB) in the postmenopausal infundibular nucleus. These data demonstrate that human menopause is associated with marked increases in hypothalamic NKB and SP gene expression. We propose that neurons containing estrogen receptor, SP, and NKB mRNAs participate in the hypothalamic circuitry regulating estrogen negative feedback in the human.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Absence of oestradiol concentration in cell nuclei of LHRH-immunoreactive neurones
Shivers, B. D., Harlan, R. E., Morrell, J. I., & Pfaff, D. W. (1983) · Nature · doi:10.1038/304345a0 · Cited by 531
LH, FSH AND SKIN TEMPERATURE DURING THE MENOPAUSAL HOT FLAS
Tataryn, I. V., Meldrum, D. R., Lu, K. H., Fruraar, A. M., & Judd, H. L. (1979) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-49-1-152 · Cited by 200
RANCE, N. E., McMULLEN, N. T., SMIALEK, J. E., PRICE, D. L., & YOUNG, W. S. (1990) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-71-1-79 · Cited by 148
Self-citation
Cited by
Drug repurposing: progress, challenges and recommendations
Pushpakom, S., Iorio, F., Eyers, P. A., Escott, K. J., Hopper, S., Wells, A., Doig, A., Guilliams, T., Latimer, J., McNamee, C., et al. (2019) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2018.168 · Cited by 4,718
Lehman, M. N., Coolen, L. M., & Goodman, R. L. (2010) · Endocrinology · doi:10.1210/en.2010-0022 · Cited by 768
Kisspeptin neurons in the arcuate nucleus of the ewe express both dynorphin A and neurokinin B
Goodman, R. L., Lehman, M. N., Smith, J. T., Coolen, L. M., de Oliveira, C. V. R., Jafarzadehshirazi, M. R., Pereira, A., Iqbal, J., Caraty, A., Ciofi, P., et al. (2007) · Endocrinology · doi:10.1210/en.2007-0961 · Cited by 692
Rometo, A. M., Krajewski, S. J., Lou Voytko, M., & Rance, N. E. (2007) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2007-0553 · Cited by 396
Rance, N. E., Dacks, P. A., Mittelman-Smith, M. A., Romanovsky, A. A., & Krajewski-Hall, S. J. (2013) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2013.07.003 · Cited by 380
Self-citationIsgor, C., Kabbaj, M., Akil, H., & Watson, S. J. (2004) · Hippocampus · doi:10.1002/hipo.10207 · Cited by 341
Burke, M. C., Letts, P. A., Krajewski, S. J., & Rance, N. E. (2006) · Journal of Comparative Neurology · doi:10.1002/cne.21086 · Cited by 280
Self-citationRance, N. E. (2009) · Peptides · doi:10.1016/j.peptides.2008.05.016 · Cited by 237
Self-citationNeurokinin B and the hypothalamic regulation of reproduction
Rance, N. E., Krajewski, S. J., Smith, M. A., Cholanian, M., & Dacks, P. A. (2010) · Brain Research · doi:10.1016/j.brainres.2010.08.059 · Cited by 203
Krajewski, S. J., Burke, M. C., Anderson, M. J., McMullen, N. T., & Rance, N. E. (2010) · Neuroscience · doi:10.1016/j.neuroscience.2009.12.053 · Cited by 178
Self-citationThe integrated hypothalamic tachykinin-kisspeptin system as a central coordinator for reproduction
Navarro, V. M., Bosch, M. A., León, S., Simavli, S., True, C., Pinilla, L., Carroll, R. S., Seminara, S. B., Tena-Sempere, M., Rønnekleiv, O. K., et al. (2015) · Endocrinology · doi:10.1210/en.2014-1651 · Cited by 128
Rance, N. E., Young, W. S., & McMullen, N. T. (1994) · Journal of Comparative Neurology · doi:10.1002/cne.903390408 · Cited by 99
Self-citationSandoval‐Guzmán, T., Stalcup, S. T., Krajewski, S. J., Voytko, M. L., & Rance, N. E. (2004) · Journal of Neuroendocrinology · doi:10.1111/j.0953-8194.2004.01143.x · Cited by 60
Self-citationHrabovszky, E., Borsay, B. Á., Rácz, K., Herczeg, L., Ciofi, P., Bloom, S. R., Ghatei, M. A., Dhillo, W. S., & Liposits, Z. (2013) · PLoS ONE · doi:10.1371/journal.pone.0072369 · Cited by 57
Krajewski-Hall, S. J., Miranda Dos Santos, F., McMullen, N. T., Blackmore, E. M., & Rance, N. E. (2019) · Endocrinology · doi:10.1210/en.2018-00934 · Cited by 42
Self-citationNeurokinin B signalling in the human reproductive axis
Topaloglu, A. K., & Semple, R. K. (2011) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2011.07.014 · Cited by 41
Vasopressin and oxytocin gene expression in the human hypothalamus
Sukhov, R. R., Walker, L. C., Rance, N. E., Price, D. L., & Young, W. S. (1993) · Journal of Comparative Neurology · doi:10.1002/cne.903370210 · Cited by 39
Göcz, B., Rumpler, É., Sárvári, M., Skrapits, K., Takács, S., Farkas, I., Csillag, V., Trinh, S. H., Bardóczi, Z., Ruska, Y., et al. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2113749119 · Cited by 35
Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
Pinto, F. M., Pintado, C. O., Pennefather, J. N., Patak, E., & Candenas, L. (2009) · Reproductive Biology and Endocrinology · doi:10.1186/1477-7827-7-77 · Cited by 29
Optogenetics studies of kisspeptin neurons
Ivanova, D., & O’Byrne, K. T. (2023) · Peptides · doi:10.1016/j.peptides.2023.170961 · Cited by 3
Metadata
- Authors
- RANCE, N. E., & YOUNG, W. S.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1991-05
- Volume
- 128
- Issue
- 5
- Pages
- 2239-2247
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2072513181
- Cited by
- 266
- References
- 3
- FWCI
- 4.70
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
