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Cited by 293

The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.

The chicken RaxL gene plays a role in the initiation of photoreceptor differentiation

Open access
Chen, C.-M. Amy, Cepko, Constance L.·2002·Development
DOI 10.1242/dev.00114
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Abstract

The paired type homeodomain gene, Rax, was previously identified as a key molecule in early eye formation in mice and humans. We report the expression patterns of two Rax family members from chicken, Rax andRaxL, and on the function of RaxL in photoreceptor development. Both Rax and RaxL are expressed in early retinal progenitor cells, with Rax being expressed at a significantly higher level than RaxL. At the time that photoreceptors begin to form, RaxL appears at a relatively high level in a subset of cells within the zone of proliferating progenitor cells. Subsequently, it is expressed in cells migrating to the photoreceptor layer, where it is highly expressed during the initial, but not late, stages of photoreceptor differentiation. To test the function of RaxL, a putative dominant-negative allele of RaxL comprising a fusion of theengrailed repressor domain and a region of RaxL(EnRaxLΔC) was introduced in vivo into the early chick eye using a retroviral vector. EnRaxLΔC, but not the dominant negative Rax(EnRaxΔC), caused a significant reduction in expression of early markers of photoreceptor cells. Examination of the transactivation activity of RaxL on a reporter construct bearing a canonical photoreceptor-specific enhancer element showed that RaxL exhibited significant activation activity, and that this activity was severely diminished in the presence of EnRaxLΔC. The effect on photoreceptor gene expression in vivo was specific in that other cell types were unaffected, as was general proliferation in the retina. The reduction in numbers of cells expressing photoreceptor markers was probably due to decreased survival of developing photoreceptor cells, as there was increased apoptosis among cells of the retina expressing dominant-negativeRaxL. We propose that RaxL plays a role in the initiation of differentiation, and also possibly commitment, of photoreceptor cells in the chicken retina.

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Metadata

Authors
Chen, C.-M. A., & Cepko, C. L.
Venue
Development
Publisher
The Company of Biologists
Published
2002-12-01
Volume
129
Issue
23
Pages
5363-5375
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
DOI
10.1242/dev.00114
OpenAlex
W2135101102
Cited by
293
References
50
FWCI
7.24
Type
journal article
Language
en
Open access
bronze

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