The DOI matches its Crossref record.
A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary
Abstract
Germline stem cells play a pivotal role in gametogenesis; yet little is known about how they are formed, how they divide to self-renew, and how these processes are genetically controlled. Here we describe the self-renewing asymmetric division of germline stem cells in the Drosophila ovarian germline, as marked by the spectrosome, a cytoplasmic structure rich in membrane skeletal proteins. The ontogeny of the spectrosome marks the lineage of germline stem cells. We identified two new groups of mutations in which the divisional asymmetry is disrupted. The first, which we refer to as ovarette (ovt) mutations, was shown to correspond to a novel class of mutations in the pumilio locus. Since pumilio is known to posttranscriptionally repress the expression of target genes at earlier stages of germ cell development, our results suggest that a similar activity is needed to maintain germ line stem cells. We have also identified a second and novel gene, piwi, whose mutations abolish germline stem cell division.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Discrete Small RNA-Generating Loci as Master Regulators of Transposon Activity in Drosophila
Brennecke, J., Aravin, A. A., Stark, A., Dus, M., Kellis, M., Sachidanandam, R., & Hannon, G. J. (2007) · Cell · doi:10.1016/j.cell.2007.01.043 · Cited by 2,524
A germline-specific class of small RNAs binds mammalian Piwi proteins
Girard, A., Sachidanandam, R., Hannon, G. J., & Carmell, M. A. (2006) · Nature · doi:10.1038/nature04917 · Cited by 1,762
PIWI-interacting small RNAs: the vanguard of genome defence
Siomi, M. C., Sato, K., Pezic, D., & Aravin, A. A. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3089 · Cited by 1,319
Deconstructing stem cell self-renewal: genetic insights into cell-cycle regulation
Orford, K. W., & Scadden, D. T. (2008) · Nature Reviews Genetics · doi:10.1038/nrg2269 · Cited by 851
Small RNAs as Guardians of the Genome
Malone, C. D., & Hannon, G. J. (2009) · Cell · doi:10.1016/j.cell.2009.01.045 · Cited by 836
Xie, T., & Spradling, A. C. (1998) · Cell · doi:10.1016/s0092-8674(00)81424-5 · Cited by 668
Self-citationThe power of the 3′ UTR: translational control and development.
Kuersten, S., & Goodwin, E. B. (2003) · Nature Reviews Genetics · doi:10.1038/nrg1125 · Cited by 530
Le Thomas, A., Rogers, A. K., Webster, A., Marinov, G. K., Liao, S. E., Perkins, E. M., Hur, J. K., Aravin, A. A., & Tóth, K. F. (2013) · Genes & Development · doi:10.1101/gad.209841.112 · Cited by 508
Saito, K., Sakaguchi, Y., Suzuki, T., Suzuki, T., Siomi, H., & Siomi, M. C. (2007) · Genes & Development · doi:10.1101/gad.1563607 · Cited by 462
Juliano, C., Wang, J., & Lin, H. (2011) · Annual Review of Genetics · doi:10.1146/annurev-genet-110410-132541 · Cited by 374
Self-citationDrosophila PIWI associates with chromatin and interacts directly with HP1a
Brower-Toland, B., Findley, S. D., Jiang, L., Liu, L., Yin, H., Dus, M., Zhou, P., Elgin, S. C. R., & Lin, H. (2007) · Genes & Development · doi:10.1101/gad.1564307 · Cited by 350
Self-citationDrosophila Brain Tumor is a translational repressor
Sonoda, J., & Wharton, R. P. (2001) · Genes & Development · doi:10.1101/gad.870801 · Cited by 329
Identification of piRNAs in the central nervous system
Lee, E. J., Banerjee, S., Zhou, H., Jammalamadaka, A., Arcila, M., Manjunath, B. S., & Kosik, K. S. (2011) · RNA · doi:10.1261/rna.2565011 · Cited by 289
Wang, S. H., & Elgin, S. C. R. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1107892109 · Cited by 232
Shen, E.-Z., Chen, H., Ozturk, A. R., Tu, S., Shirayama, M., Tang, W., Ding, Y.-H., Dai, S.-Y., Weng, Z., & Mello, C. C. (2018) · Cell · doi:10.1016/j.cell.2018.02.002 · Cited by 226
Human Pumilio Proteins Recruit Multiple Deadenylases to Efficiently Repress Messenger RNAs
Van Etten, J., Schagat, T. L., Hrit, J., Weidmann, C. A., Brumbaugh, J., Coon, J. J., & Goldstrohm, A. C. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.373522 · Cited by 205
Morris, A. R., Mukherjee, N., & Keene, J. D. (2008) · Molecular and Cellular Biology · doi:10.1128/mcb.00155-08 · Cited by 155
Mechanisms of asymmetric cell division during animal development
Knoblich, J. A. (1997) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(97)80085-3 · Cited by 87
Zhang, C., Robinson, B. S., Xu, W., Yang, L., Yao, B., Zhao, H., Byun, P. K., Jin, P., Veraksa, A., & Moberg, K. H. (2015) · Developmental Cell · doi:10.1016/j.devcel.2015.05.010 · Cited by 70
Performance of the Cas9 Nickase System in Drosophila melanogaster
Ren, X., Yang, Z., Mao, D., Chang, Z., Qiao, H.-H., Wang, X., Sun, J., Hu, Q., Cui, Y., Liu, L.-P., et al. (2014) · G3 Genes|Genomes|Genetics · doi:10.1534/g3.114.013821 · Cited by 40
Adaptive Evolution Targets a piRNA Precursor Transcription Network
Parhad, S. S., Yu, T., Zhang, G., Rice, N. P., Weng, Z., & Theurkauf, W. E. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.01.109 · Cited by 28
RG motifs promote piRNA-mediated gene silencing in C. elegans
Wallis, D. C., & Phillips, C. M. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf1331 · Cited by 3
Adaptive evolution targets a piRNA precursor transcription network
Parhad, S. S., Yu, T., Zhang, G., Rice, N. P., Weng, Z., & Theurkauf, W. E. (2019) · doi:10.1101/678227 · Cited by 3
Metadata
- Authors
- Lin, H., & Spradling, A. C.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1997-06-15
- Volume
- 124
- Issue
- 12
- Pages
- 2463-2476
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2100050856
- Cited by
- 698
- References
- 0
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
