Sequential expression and role of Hu RNA-binding proteins during neurogenesis
Abstract
We have identified three avian (chicken) Hu/elav family RNA-binding protein genes. cHuD and cHuC are expressed specifically in neurons of both the central and peripheral nervous systems. Although cHuA is expressed in a wide variety of tissues, including neurogenic precursor cells, it is transiently down-regulated, and is then re-expressed in maturing neurons. Misexpression of cHuD in cultured neural crest cells results in a dramatic increase in the proportion of cells exhibiting neuronal morphology, molecular markers for neurons, and neurotrophin dependence. These data confirm that cHuD protein is involved in regulating neuronal differentiation.
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HuD is one of the earliest markers of the neuronal phenotype.
“Converging findings suggest that HuD is one of the earliest markers of the neuronal phenotype (Good 1995; Okano and Darnell 1997; Wakamatsu and Weston 1997; Hambardzumyan et al. 2009).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Wakamatsu and Weston 1997
HuD-dependent neurogenesis requires commitment of cells to the neuronal lineage.
“Moreover, HuD-dependent neurogenesis requires commitment of cells to the neuronal lineage since ectopic expression of HuD in nonneuronal cells does not result in neuronal differentiation (Wakamatsu and Weston 1997).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Wakamatsu and Weston 1997
HuD has an essential role in terminal differentiation, namely dendritic and axonal outgrowth.
“Studies in which HuD levels were down-regulated or over-expressed in cultured cells have revealed an essential role for HuD in terminal differentiation, namely dendritic and axonal outgrowth (Wakamatsu and Weston 1997; Aranda-Abreu et al. 1999; Mobarak et al. 2000; Anderson et al. 2001; Hubers et al. 2010).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Wakamatsu and Weston 1997
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- Authors
- Wakamatsu, Y., & Weston, J. A.
- Affiliations
- University of Oregon (US)
- Corresponding author
- Wakamatsu, Yoshio
- Weston, James A.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1997-09-01
- Volume
- 124
- Issue
- 17
- Pages
- 3449-3460
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2183024399
- Cited by
- 240
- References
- 4
- Citations by year
- 2026: 3 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 5 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 4 · 2015: 9 · 2014: 6 · 2013: 9 · 2012: 12
- FWCI
- 4.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ns029438-07)
- National Institute of Neurological Disorders and Stroke (NS-29438)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- HUD, HuC, neurogenesis, neuronal differentiation, neural crest cells, chickens, RNA-binding proteins
- MeSH terms
- Amino Acid Sequence, Animals, Cell Differentiation, Cell Division, Cells, Cultured, Chick Embryo, Coturnix, Genetic Markers, Humans, Molecular Sequence Data, Nerve Tissue Proteins, Nervous System, Neural Crest, Neurons, RNA, Messenger, Species Specificity, Transfection, 3T3 Cells, RNA-Binding Proteins, Apoptosis, Sequence Homology, Amino Acid, In Situ Hybridization, Gene Expression Regulation, Developmental, Mice, ELAV Proteins
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