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Short communication: Influence of various proteolytic sources during fermentation of reconstituted corn grain silages
Open accessAbstract
The objective of this study was to determine the contribution of corn kernel enzymes, bacteria, fungi, and fermentation end-products (main acids and ethanol) to protein solubilization during fermentation of reconstituted corn grain silage. Flint corn kernels were ground (5-mm sieve), rehydrated to 32% of moisture, and treated with no additives (control), gamma irradiation (32 kGy), gamma irradiation + fermentation end-products (1% of lactic acid, 0.3% of acetic acid, and 0.7% of ethanol, as fed), and natamycin (1% as fed). Treated grains were ensiled in nylon-polyethylene bags and stored for 90 d. Protein solubilization was calculated for each treatment and the contributions of proteolytic sources were determined. Bacterial activity was the main contributor to proteolysis (60%) followed by corn kernel enzymes (30%), whereas fungi and fermentation end-products had only minor contributions (∼5% each).
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“In rehydrated corn grain silages, Junges et al. [45] reported that bacterial enzymes were primarily responsible for proteolysis (60%), followed by plant proteases (30%).”
From The Effects of Short-Time Delayed Sealing on Fermentation, Aerobic Stability and Chemical Composition on Maize Silages (2023) · cites this paper as [45]
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Metadata
- Authors
- Junges, D., Morais, G., Spoto, M. H. F., Santos, P. S., Adesogan, A. T., Nussio, L. G., & Daniel, J. L. P.
- Affiliations
- Universidade de São Paulo (BR)
- Instituto de Pesquisas Energéticas e Nucleares (BR)
- University of Florida (US)
- Universidade Estadual de Maringá (BR)
- Corresponding author
- Daniel, J.L.P.
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2017-11
- Volume
- 100
- Issue
- 11
- Pages
- 9048-9051
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2754070343
- Cited by
- 157
- References
- 30
- Citations by year
- 2026: 9 · 2025: 17 · 2024: 14 · 2023: 37 · 2022: 21 · 2021: 20 · 2020: 21 · 2019: 14 · 2018: 4
- FWCI
- 18.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Keywords
- protein solubilization, proteolysis, silage additives, gamma irradiation, natamycin, lactic acid, acetic acid, ethanol, fungal activity, flint corn
- MeSH terms
- Animals, Bacteria, Zea mays, Fermentation, Fungi, Hydrogen-Ion Concentration, Plant Proteins, Silage, Acetic Acid, Proteolysis
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