Cited by 336Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchNeuroinflammation and Neurodegeneration Mechanisms

Intraneuronal Aβ accumulation precedes plaque formation in β-amyloid precursor protein and presenilin-1 double-transgenic mice

Wirths, Oliver, Multhaup, Gerd, Czech, Christian, Blanchard, Véronique, Moussaoui, Saliha, Tremp, Günter, Pradier, Laurent, Beyreuther, Konrad +1 more·2001·Neuroscience Letters

Abstract

beta-Amyloid peptides are key molecules that are involved in the pathology of Alzheimer's disease (AD). The source and place of the neurotoxic action of Abeta, however, is still a matter of controversial debates. In the present report, we studied the neuropathological events in a transgenic mouse model expressing human mutant beta-amyloid precursor protein and human mutant presenilin-1 in neurons. Western blot and immunohistochemical analysis revealed that intracellular Abeta staining preceded plaque deposition, which started in the hippocampal formation. At later stages, many neuritic Abeta positive plaques were found in all cortical, hippocampal and many other brain areas. Interestingly, intraneuronal Abeta staining was no longer detected in the brain of aged double-transgenic mice, which correlates with the typical neuropathology in the brain of chronic AD patients.

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Crossref · 10.1016/s0304-3940(01)01876-6

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Authors
Wirths, O., Multhaup, G., Czech, C., Blanchard, V., Moussaoui, S., Tremp, G., Pradier, L., Beyreuther, K., & Bayer, T. A.
Affiliations
  • University of Bonn (DE)
  • Sanofi (France) (FR)
Corresponding author
Bayer, Thomas A
Venue
Neuroscience Letters
Publisher
Elsevier BV
Published
2001-06
Volume
306
Issue
1-2
Pages
116-120
ISSN
0304-3940 (ISSN-L)
OpenAlex
W1975963996
Cited by
336
References
19
Citations by year
2026: 2 · 2025: 3 · 2024: 8 · 2023: 6 · 2022: 7 · 2021: 7 · 2020: 9 · 2019: 14 · 2018: 8 · 2017: 10 · 2016: 11 · 2015: 17 · 2014: 12 · 2013: 15 · 2012: 16
FWCI
11.16
Type
journal article
Language
en
Open access
closed
Locations
  • Neuroscience Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
amyloid-beta, amyloid plaques, Alzheimer's disease, APP/PS1 double transgenic mice, presenilin 1, amyloid precursor protein, hippocampus, neurotoxicity
MeSH terms
Alzheimer Disease, Animals, Brain, Cerebral Cortex, Gliosis, Hippocampus, Humans, Immunohistochemistry, Membrane Proteins, Mice, Transgenic, Mutation, Neurons, Amyloid beta-Peptides, Amyloid beta-Protein Precursor, Mice, Presenilin-1, Plaque, Amyloid

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