Engineering Organoid Vascularization
Open accessAbstract
The development of increasingly biomimetic human tissue analogues has been a long-standing goal in two important biomedical applications: drug discovery and regenerative medicine. In seeking to understand the safety and effectiveness of newly developed pharmacological therapies and replacement tissues for severely injured non-regenerating tissues and organs, there remains a tremendous unmet need in generating tissues with both functional complexity and scale. Over the last decade, the advent of organoids has demonstrated that cells have the ability to reorganize into complex tissue-specific structures given minimal inductive factors. However a major limitation in achieving truly in-vivo-like functionality has been the lack of structured organization and reasonable tissue size. In-vivo, developing tissues are interpenetrated by and interact with a complex network of vasculature which allows not only oxygen, nutrient and waste exchange, but also allow for inductive biochemical exchange and a structural template for growth. Conversely, in-vitro, this aspect of organoid development has remained largely missing, suggesting that these may be the critical cues required for large-scale and more reproducible tissue organization. Here, we review recent technical progress in generating in-vitro vasculature, and seek to provide a framework for understanding how such technologies, together with theoretical and developmentally inspired insights, can be harnessed to enhance next generation organoid development.
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Kidney organoids have been cultured in an OoC device that enables fluid flow to simulate shear stress and the generation of a vascular network.
“Kidney organoids have been cultured in an OoC device which enables fluid flow to simulate shear stress107 and the generation of a vascular network108.”
From Organoids (2022) · cites this paper as [107]
The ‘Induced Angiogenesis in Engineered Tissues’ section describes a vascular network forming in a microfluidic chip containing human lung fibroblast spheroids. It does not report a vascular network generated with kidney organoids in an organ-on-a-chip device; the citation to this paper is attached to the network-generation part of the sentence, not the shear-stress part. No page number is available in our copy. (LLM, conf=0.83)
Experimental — accuracy not yet measuredThe presence of fluid flow enhances kidney organoids' maturation and favors their vascularization in vitro.
“A more recent example shows how the presence of fluid flow enhances kidney organoids’ maturation and favors their vascularization in vitro107.”
From Organoids (2022) · cites this paper as [107]
Our copy does not report that fluid flow enhances kidney organoid maturation or vascularization in vitro. The ‘Induced Angiogenesis in Engineered Tissues’ section describes vascularization of lung fibroblast spheroids in a microfluidic chip, while the ‘Organoid Vascularization via in vivo Organoid Transplantation’ section discusses vascularization after transplantation; neither addresses the claimed kidney-organoid result. Searches for kidney organoids, fluid flow, and perfusion did not locate it. (LLM, conf=0.76)
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Cites
Designing materials for biology and medicine
Langer, R., & Tirrell, D. A. (2004) · Nature · doi:10.1038/nature02388 · Cited by 3,198 · see citing works
Modeling Development and Disease with Organoids
Clevers, H. (2016) · Cell · doi:10.1016/j.cell.2016.05.082 · Cited by 3,196 · see citing works
Capturing complex 3D tissue physiology in vitro
Griffith, L. G., & Swartz, M. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1858 · Cited by 2,274 · see citing works
Vascularized and functional human liver from an iPSC-derived organ bud transplant
Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170 · see citing works
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Miller, J. S., Stevens, K. R., Yang, M. T., Baker, B. M., Nguyen, D.-H. T., Cohen, D. M., Toro, E., Chen, A. A., Galie, P. A., Yu, X., et al. (2012) · Nature Materials · doi:10.1038/nmat3357 · Cited by 1,917 · see citing works
Matrigel: Basement membrane matrix with biological activity
Kleinman, H. K., & Martin, G. R. (2005) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2005.05.004 · Cited by 1,673 · see citing works
Three-dimensional bioprinting of thick vascularized tissues
Kolesky, D. B., Homan, K. A., Skylar-Scott, M. A., & Lewis, J. A. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521342113 · Cited by 1,492 · see citing works
Designer matrices for intestinal stem cell and organoid culture
Gjorevski, N., Sachs, N., Manfrin, A., Giger, S., Bragina, M. E., Ordóñez-Morán, P., Clevers, H., & Lutolf, M. P. (2016) · Nature · doi:10.1038/nature20168 · Cited by 1,426 · see citing works
Designing materials to direct stem-cell fate
Lütolf, M. P., Gilbert, P. M., & Blau, H. M. (2009) · Nature · doi:10.1038/nature08602 · Cited by 1,389 · see citing works
An in vivo model of functional and vascularized human brain organoids
Mansour, A. A., Gonçalves, J. T., Bloyd, C. W., Li, H., Fernandes, S., Quang, D., Johnston, S., Parylak, S. L., Jin, X., & Gage, F. H. (2018) · Nature Biotechnology · doi:10.1038/nbt.4127 · Cited by 1,211 · see citing works
Human cerebral organoids recapitulate gene expression programs of fetal neocortex development
Camp, J. G., Badsha, F., Florio, M., Kanton, S., Gerber, T., Wilsch-Bräuninger, M., Lewitus, E., Sykes, A., Hevers, W., Lancaster, M., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1520760112 · Cited by 1,130 · see citing works
A functional CFTR assay using primary cystic fibrosis intestinal organoids
Dekkers, J. F., Wiegerinck, C. L., de Jonge, H. R., Bronsveld, I., Janssens, H. M., de Winter-de Groot, K. M., Brandsma, A. M., de Jong, N. W. M., Bijvelds, M. J. C., Scholte, B. J., et al. (2013) · Nature Medicine · doi:10.1038/nm.3201 · Cited by 999 · see citing works
Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip
Zhang, Y. S., Arneri, A., Bersini, S., Shin, S.-R., Zhu, K., Goli-Malekabadi, Z., Aleman, J., Colosi, C., Busignani, F., Dell’Erba, V., et al. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.09.003 · Cited by 957 · see citing works
Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs
Bertassoni, L. E., Cecconi, M., Manoharan, V., Nikkhah, M., Hjortnaes, J., Cristino, A. L., Barabaschi, G., Demarchi, D., Dokmeci, M. R., Yang, Y., et al. (2014) · Lab Chip · doi:10.1039/c4lc00030g · Cited by 944 · see citing works
Direct 3D bioprinting of perfusable vascular constructs using a blend bioink
Jia, W., Gungor-Ozkerim, P. S., Zhang, Y. S., Yue, K., Zhu, K., Liu, W., Pi, Q., Byambaa, B., Dokmeci, M. R., Shin, S. R., et al. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.07.038 · Cited by 922 · see citing works
A comprehensive review on droplet-based bioprinting: Past, present and future
Gudapati, H., Dey, M., & Ozbolat, I. T. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.06.012 · Cited by 867 · see citing works
Omnidirectional Printing of 3D Microvascular Networks
Wu, W., DeConinck, A., & Lewis, J. A. (2011) · Advanced Materials · doi:10.1002/adma.201004625 · Cited by 819 · see citing works
Organotypic vasculature: From descriptive heterogeneity to functional pathophysiology
Augustin, H. G., & Koh, G. Y. (2017) · Science · doi:10.1126/science.aal2379 · Cited by 762 · see citing works
Yan, H. H. N., Siu, H. C., Law, S., Ho, S. L., Yue, S. S. K., Tsui, W. Y., Chan, D., Chan, A. S., Ma, S., Lam, K. O., et al. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.09.016 · Cited by 724 · see citing works
Thermal Inkjet Printing in Tissue Engineering and Regenerative Medicine
Cui, X., Boland, T., DʼLima, D. D., & Lotz, M. (2012) · Recent Patents on Drug Delivery & Formulation · doi:10.2174/187221112800672949 · Cited by 587 · see citing works
Direct 3D bioprinting of prevascularized tissue constructs with complex microarchitecture
Zhu, W. Z., Qu, X., Zhu, J., Ma, X., Patel, S. H., Liu, J., Wang, P., Lai, C. S. E., Gou, M., Xu, Y., et al. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.01.042 · Cited by 568 · see citing works
Synthetic hydrogels for human intestinal organoid generation and colonic wound repair
Cruz-Acuña, R., Quirós, M., Farkas, A. E., Dedhia, P. H., Huang, S., Siuda, D., García-Hernández, V., Miller, A. J., Spence, J. R., Nusrat, A., et al. (2017) · Nature Cell Biology · doi:10.1038/ncb3632 · Cited by 558 · see citing works
An in vivo model of human small intestine using pluripotent stem cells
Watson, C. L., Mahe, M. M., Múnera, J., Howell, J., Sundaram, N., Poling, H. M., Schweitzer, J. I., Vallance, J. E., Mayhew, C., Sun, Y., et al. (2014) · Nature Medicine · doi:10.1038/nm.3737 · Cited by 550 · see citing works
Dekkers, J. F., Berkers, G., Kruisselbrink, E., Vonk, A., de Jonge, H. R., Janssens, H. M., Bronsveld, I., van de Graaf, E. A., Nieuwenhuis, E. E. S., Houwen, R. H. J., et al. (2016) · Science Translational Medicine · doi:10.1126/scitranslmed.aad8278 · Cited by 548 · see citing works
Delivery of human fibroblast cells by piezoelectric drop-on-demand inkjet printing
Saunders, R., Gough, J. E., & Derby, B. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.09.032 · Cited by 495 · see citing works
Fabrication of microfluidic hydrogels using molded gelatin as a sacrificial element
Golden, A. P., & Tien, J. (2007) · Lab on a Chip · doi:10.1039/b618409j · Cited by 479 · see citing works
Bioprinting Technology: A Current State-of-the-Art Review
Dababneh, A. B., & Ozbolat, I. T. (2014) · Journal of Manufacturing Science and Engineering · doi:10.1115/1.4028512 · Cited by 427 · see citing works
Generation of human vascularized brain organoids
Pham, M. T., Pollock, K., Rose, M. D., Cary, W., Stewart, H., Zhou, P., Nolta, J., & Waldau, B. (2018) · NeuroReport · doi:10.1097/wnr.0000000000001014 · Cited by 412 · see citing works
Scaffold‐free inkjet printing of three‐dimensional zigzag cellular tubes
Xu, C., Chai, W., Huang, Y. H., & Markwald, R. R. (2012) · Biotechnology and Bioengineering · doi:10.1002/bit.24591 · Cited by 358 · see citing works
Freeform inkjet printing of cellular structures with bifurcations
Christensen, K., Xu, C., Chai, W., Zhang, Z., Fu, J., & Huang, Y. H. (2014) · Biotechnology and Bioengineering · doi:10.1002/bit.25501 · Cited by 352 · see citing works
Dishing out mini-brains: Current progress and future prospects in brain organoid research
Kelava, I., & Lancaster, M. A. (2016) · Developmental Biology · doi:10.1016/j.ydbio.2016.06.037 · Cited by 309 · see citing works
3D printing of microscopic bacterial communities
Connell, J. L., Ritschdorff, E. T., Whiteley, M., & Shear, J. B. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1309729110 · Cited by 305 · see citing works
Radisic, M., Deen, W. M., Langer, R., & Vunjak‐Novakovic, G. (2004) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00787.2004 · Cited by 269 · see citing works
Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device
Nashimoto, Y., Hayashi, T., Kunita, I., Nakamasu, A., Torisawa, Y.-S., Nakayama, M., Takigawa-Imamura, H., Kotera, H., Nishiyama, K., Miura, T., et al. (2017) · Integrative Biology · doi:10.1039/c7ib00024c · Cited by 266 · see citing works
McMurtrey, R. J. (2015) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2015.0375 · Cited by 248 · see citing works
Transcriptional and epigenomic landscapes of CNS and non-CNS vascular endothelial cells
Sabbagh, M. F., Heng, J. S., Luo, C., Castanon, R. G., Nery, J. R., Rattner, A., Goff, L. A., Ecker, J. R., & Nathans, J. (2018) · eLife · doi:10.7554/elife.36187 · Cited by 245 · see citing works
3D niche microarrays for systems-level analyses of cell fate
Ranga, A., Gobaa, S., Okawa, Y., Mosiewicz, K., Negro, A., & Lutolf, M. P. (2014) · Nature Communications · doi:10.1038/ncomms5324 · Cited by 238 · see citing works
Self-citation3D printing of biomimetic microstructures for cancer cell migration
Huang, T. Q., Qu, X., Liu, J., & Chen, S. (2013) · Biomedical Microdevices · doi:10.1007/s10544-013-9812-6 · Cited by 238 · see citing works
Neural tube morphogenesis in synthetic 3D microenvironments
Ranga, A., Girgin, M., Meinhardt, A., Eberle, D., Caiazzo, M., Tanaka, E. M., & Lutolf, M. P. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1603529113 · Cited by 221 · see citing works
Self-citationKloxin, A. M., Tibbitt, M. W., Kasko, A. M., Fairbairn, J. A., & Anseth, K. S. (2009) · Advanced Materials · doi:10.1002/adma.200900917 · Cited by 215 · see citing works
3D Reconstitution of the Patterned Neural Tube from Embryonic Stem Cells
Meinhardt, A., Eberle, D., Tazaki, A., Ranga, A., Niesche, M., Wilsch‐Bräuninger, M., Stec, A., Schackert, G., Lütolf, M. P., & Tanaka, E. M. (2014) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.09.020 · Cited by 214 · see citing works
Self-citationThree-Dimensional Analysis of Vascular Development in the Mouse Embryo
Walls, J. R., Coultas, L., Rossant, J., & Henkelman, R. M. (2008) · PLoS ONE · doi:10.1371/journal.pone.0002853 · Cited by 209 · see citing works
Piezoelectric inkjet printing of polymers: Stem cell patterning on polymer substrates
Kim, J. D., Choi, J. S., Kim, B. S., Choi, Y. C., & Cho, Y. W. (2010) · Polymer · doi:10.1016/j.polymer.2010.03.038 · Cited by 208 · see citing works
Intracerebral grafting: A tool for the neurobiologist
Gage, F. H., & Fisher, L. J. (1991) · Neuron · doi:10.1016/0896-6273(91)90116-h · Cited by 198 · see citing works
Drug discovery through stem cell-based organoid models
Ranga, A., Gjorevski, N., & Lutolf, M. P. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.02.006 · Cited by 195 · see citing works
Self-citationIn Situ Patterning of Microfluidic Networks in 3D Cell‐Laden Hydrogels
Brandenberg, N., & Lütolf, M. P. (2016) · Advanced Materials · doi:10.1002/adma.201601099 · Cited by 192 · see citing works
Kunz‐Schughart, L. A., Schroeder, J. A., Wondrak, M., van Rey, F., Lehle, K., Hofstaedter, F., & Wheatley, D. N. (2006) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00248.2005 · Cited by 184 · see citing works
Biomimetic design of microfluidic manifolds based on a generalised Murray's law
Emerson, D. R., Cieślicki, K., Gu, X.-J., & Barber, R. W. (2006) · Lab on a Chip · doi:10.1039/b516975e · Cited by 166 · see citing works
Ovsianikov, A., Mironov, V., Stampfl, J., & Liska, R. (2012) · Expert Review of Medical Devices · doi:10.1586/erd.12.48 · Cited by 160 · see citing works
Vascularization of three-dimensional engineered tissues for regenerative medicine applications
Kim, J. J., Hou, L., & Huang, N. F. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.06.001 · Cited by 156 · see citing works
Raimondi, M. T., Eaton, S. M., Nava, M. M., Laganà, M., Cerullo, G., & Osellame, R. (2012) · Journal of Applied Biomaterials & Functional Materials · doi:10.5301/jabfm.2012.9278 · Cited by 151 · see citing works
Laser Fabrication of 3D Gelatin Scaffolds for the Generation of Bioartificial Tissues
Ovsianikov, A., Deiwick, A., Vlierberghe, S. V., Pflaum, M., Wilhelmi, M. H., Dubruel, P., & Chichkov, B. N. (2011) · Materials · doi:10.3390/ma4010288 · Cited by 150 · see citing works
Sacchi, V., Mittermayr, R., Hartinger, J., Martino, M. M., Lorentz, K. M., Wolbank, S., Hofmann, A. T., Largo, R. A., Marschall, J. S., Groppa, E., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1404605111 · Cited by 149 · see citing works
Applegate, M. B., Coburn, J. M., Partlow, B. P., Moreau, J. E., Mondia, J. P., Marelli, B., Kaplan, D. L., & Omenetto, F. G. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1509405112 · Cited by 147 · see citing works
Laser Photoablation of Guidance Microchannels into Hydrogels Directs Cell Growth in Three Dimensions
Sarig-Nadir, O., Livnat, N., Zajdman, R., Shoham, S., & Seliktar, D. (2009) · Biophysical Journal · doi:10.1016/j.bpj.2009.03.019 · Cited by 143 · see citing works
Patterned prevascularised tissue constructs by assembly of polyelectrolyte hydrogel fibres
Leong, M. F., Toh, J. K. C., Du, C., Narayanan, K., Lu, H., Lim, T. C., Wan, A. C. A., & Ying, J. Y. (2013) · Nature Communications · doi:10.1038/ncomms3353 · Cited by 143 · see citing works
Electro‐Assisted Bioprinting of Low‐Concentration GelMA Microdroplets
Xie, M., Gao, Q., Zhao, H., Nie, J., Fu, Z., Wang, H., Chen, L., Shao, L., Fu, J., Chen, Z., et al. (2018) · Small · doi:10.1002/smll.201804216 · Cited by 137 · see citing works
Ehrbar, M., Zeisberger, S. M., Raeber, G. P., Hubbell, J. A., Schnell, C., & Zisch, A. H. (2008) · Biomaterials · doi:10.1016/j.biomaterials.2007.12.002 · Cited by 136 · see citing works
3D-printed fluidic networks as vasculature for engineered tissue
Kinstlinger, I. S., & Miller, J. S. (2016) · Lab on a Chip · doi:10.1039/c6lc00193a · Cited by 132 · see citing works
Aqueous multiphoton lithography with multifunctional silk-centred bio-resists
Sun, Y., Li, Q., Sun, S., Huang, J., Zheng, B., Chen, Q., Shao, Z., & Sun, H. (2015) · Nature Communications · doi:10.1038/ncomms9612 · Cited by 130 · see citing works
Oxygen Transport in the Microcirculation and Its Regulation
Pittman, R. N. (2012) · Microcirculation · doi:10.1111/micc.12017 · Cited by 129 · see citing works
Tibbitt, M., Kloxin, A. M., Dyamenahalli, K., & Anseth, K. (2010) · Soft Matter · doi:10.1039/c0sm00174k · Cited by 123 · see citing works
Hynds, R. E., & Giangreco, A. (2012) · Stem Cells · doi:10.1002/stem.1290 · Cited by 105 · see citing works
Nishiyama, Y., Henmi, C., Iwanaga, S., Nakagawa, H., Yamaguchi, K., Akita, K., Mochizuki, S., Takiura, K., & Nakamura, M. (2008) · Journal of Imaging Science and Technology · doi:10.2352/j.imagingsci.technol.(2008)52:6(060201 · Cited by 94 · see citing works
Soft Polymers for Building up Small and Smallest Blood Supplying Systems by Stereolithography
Meyer, W., Engelhardt, S., Novosel, E. C., Elling, B., Wegener, M., & Krüger, H. (2012) · Journal of Functional Biomaterials · doi:10.3390/jfb3020257 · Cited by 85 · see citing works
Groot, K. M. D. W., Janssens, H. M., van Uum, R. T., Dekkers, J. F., Berkers, G., Vonk, A. M., Kruisselbrink, E., Oppelaar, H., Vries, R. G. J., Clevers, H. C., et al. (2018) · European Respiratory Journal · doi:10.1183/13993003.02529-2017 · Cited by 82 · see citing works
Sooppan, R., Paulsen, S. J., Han, J. J., Ta, A. H., Dinh, P. V., Gaffey, A. C., Venkataraman, C. M., Trubelja, A., Hung, G., Miller, J. S., et al. (2015) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2015.0239 · Cited by 64 · see citing works
Roudsari, L. C., Jeffs, S. E., Witt, A. S., Gill, B. J., & West, J. L. (2016) · Scientific Reports · doi:10.1038/srep32726 · Cited by 64 · see citing works
Li, S., Liu, Y.-Y., Liu, L., & Hu, Q. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b07725 · Cited by 61 · see citing works
Sala, A., Hänseler, P., Ranga, A., Lütolf, M. P., Vörös, J., Ehrbar, M., & Weber, F. E. (2011) · Integrative Biology · doi:10.1039/c1ib00045d · Cited by 52 · see citing works
Self-citationGuided Homing of Cells in Multi‐Photon Microfabricated Bioscaffolds
Skylar‐Scott, M. A., Liu, M., Wu, Y., Dixit, A. C., & Yanik, M. F. (2016) · Advanced Healthcare Materials · doi:10.1002/adhm.201600082 · Cited by 49 · see citing works
Xu, C., Zhang, Z., Christensen, K., Huang, Y. H., Fu, J., & Markwald, R. R. (2014) · Journal of Manufacturing Science and Engineering · doi:10.1115/1.4028578 · Cited by 48 · see citing works
Fabrication of multilayered vascular tissues using microfluidic agarose hydrogel platforms
Kinoshita, K., Iwase, M., Yamada, M., Yajima, Y., & Seki, M. (2016) · Biotechnology Journal · doi:10.1002/biot.201600083 · Cited by 46 · see citing works
Huber, B., Engelhardt, S., Meyer, W., Krüger, H., Wenz, A., Schönhaar, V., Tovar, G. E. M., Kluger, P. J., & Borchers, K. (2016) · Journal of Functional Biomaterials · doi:10.3390/jfb7020011 · Cited by 43 · see citing works
A design principle for vascular beds: the effects of complex blood rheology
Alarcón, T., Byrne, H. M., & Maini, P. K. (2005) · Microvascular Research · doi:10.1016/j.mvr.2005.02.002 · Cited by 38 · see citing works
Design of vascular networks: A mathematical model approach
Yang, J., & Wang, Y. (2012) · International Journal for Numerical Methods in Biomedical Engineering · doi:10.1002/cnm.2534 · Cited by 36 · see citing works
Three dimensional microstructuring of biopolymers by femtosecond laser irradiation
Oujja, M., Pérez, S., Fadeeva, E., Koch, J., Chichkov, B. N., & Castillejo, M. (2009) · Applied Physics Letters · doi:10.1063/1.3274127 · Cited by 33 · see citing works
Biomimetic Design of Artificial Micro-vasculatures for Tissue Engineering
Barber, R. W., & Emerson, D. R. (2010) · Alternatives to Laboratory Animals · doi:10.1177/026119291003801s02 · Cited by 28 · see citing works
Construction of Large-Volume Tissue Mimics with 3D Functional Vascular Networks
Kang, T.-Y., Hong, J. M., Jung, J. W., Kang, H., & Cho, D. (2016) · PLoS ONE · doi:10.1371/journal.pone.0156529 · Cited by 17 · see citing works
Design and Assessment of a Microfluidic Network System for Oxygen Transport in Engineered Tissue
Kang, T.-Y., Hong, J. M., Jung, J. W., Yoo, J. J., & Cho, D. (2012) · Langmuir · doi:10.1021/la303552m · Cited by 14 · see citing works
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Zhao, Z., Chen, X., Dowbaj, A. M., Sljukic, A., Bratlie, K., Lin, L., Fong, E. L. S., Balachander, G. M., Chen, Z., Soragni, A., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00174-y · Cited by 1,001 · see citing works
Basic and Therapeutic Aspects of Angiogenesis Updated
Eelen, G., Treps, L., Li, X., & Carmeliet, P. (2020) · Circulation Research · doi:10.1161/circresaha.120.316851 · Cited by 571 · see citing works
Engineering Stem Cell Self-organization to Build Better Organoids
Brassard, J. A., & Lütolf, M. P. (2019) · Cell stem cell · doi:10.1016/j.stem.2019.05.005 · Cited by 379 · see citing works
Vascularized organoids on a chip: strategies for engineering organoids with functional vasculature
Zhang, S., Wan, Z., & Kamm, R. D. (2021) · Lab on a Chip · doi:10.1039/d0lc01186j · Cited by 317 · see citing works
Tumor organoids: applications in cancer modeling and potentials in precision medicine
Xu, H., Jiao, D., Liu, A., & Wu, K. (2022) · Journal of Hematology & Oncology · doi:10.1186/s13045-022-01278-4 · Cited by 286 · see citing works
Bioengineering Approaches for the Advanced Organoid Research
Yi, S. A., Zhang, Y., Rathnam, C., Pongkulapa, T., & Lee, K. (2021) · Advanced Materials · doi:10.1002/adma.202007949 · Cited by 218 · see citing works
Advanced Bottom‐Up Engineering of Living Architectures
Gaspar, V. M., Lavrador, P., Borges, J., Oliveira, M. B., & Mano, J. F. (2019) · Advanced Materials · doi:10.1002/adma.201903975 · Cited by 204 · see citing works
The horizon of bone organoid: A perspective on construction and application
Chen, S., Chen, X., Geng, Z., & Su, J. (2022) · Bioactive Materials · doi:10.1016/j.bioactmat.2022.01.048 · Cited by 197 · see citing works
Engineering Vascularized Organoid-on-a-Chip Models
Shirure, V. S., Hughes, C. C. W., & George, S. C. (2021) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-090120-094330 · Cited by 197 · see citing works
Advances in Engineering Human Tissue Models
Moysidou, C., Barberio, C., & Owens, R. M. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.620962 · Cited by 182 · see citing works
Mechanisms of radiation-induced endothelium damage: Emerging models and technologies
Wijerathne, H., Langston, J. C., Yang, Q., Sun, S., Miyamoto, C. T., Kilpatrick, L. E., & Kiani, M. F. (2021) · Radiotherapy and Oncology · doi:10.1016/j.radonc.2021.02.007 · Cited by 175 · see citing works
Review on the Vascularization of Organoids and Organoids-on-a-Chip
Zhao, X., Xu, Z., Xiao, L., Shi, T., Xiao, H., Wang, Y., Li, Y., Xue, F., & Zeng, W. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.637048 · Cited by 157 · see citing works
Patient-Derived Organoids for Precision Cancer Immunotherapy
Grönholm, M., Feodoroff, M., Antignani, G., Martins, B., Hamdan, F., & Cerullo, V. (2021) · Cancer Research · doi:10.1158/0008-5472.can-20-4026 · Cited by 146 · see citing works
Large-scale perfused tissues via synthetic 3D soft microfluidics
Grebenyuk, S. E., Fattah, A. R. A., Kumar, M., Toprakhisar, B., Rustandi, G., Vananroye, A., Salmon, I., Verfaillie, C. M., Grillo, M. P., & Ranga, A. (2023) · Nature Communications · doi:10.1038/s41467-022-35619-1 · Cited by 153 · see citing works
Self-citationOrganoids as host models for infection biology – a review of methods
Aguilar, C., da Silva, M. A., Saraiva, M., Neyazi, M., Olsson, I. A. S., & Bartfeld, S. (2021) · Experimental & Molecular Medicine · doi:10.1038/s12276-021-00629-4 · Cited by 128 · see citing works
Harane, S. E., Zidi, B., Harane, N. E., Krause, K., Matthes, T., & Preynat‐Seauve, O. (2023) · Cells · doi:10.3390/cells12071001 · Cited by 132 · see citing works
Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine
Fonseca, A. C., Melchels, F. P. W., Ferreira, M. J. S., Moxon, S. R., Potjewyd, G., Dargaville, T. R., Kimber, S. J., & Domingos, M. A. N. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00342 · Cited by 129 · see citing works
Advances in Spheroids and Organoids on a Chip
Fang, G., Chen, Y., Lu, H., & Jin, D. (2023) · Advanced Functional Materials · doi:10.1002/adfm.202215043 · Cited by 127 · see citing works
Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System
Ahn, Y.-L., An, J.-H., Yang, H.-J., Lee, D. G., Kim, J., Koh, H., Park, Y.-H., Song, B., Sim, B.-W., Lee, H. J., et al. (2021) · Cells · doi:10.3390/cells10082036 · Cited by 105 · see citing works
In Vitro Strategies to Vascularize 3D Physiologically Relevant Models
Dellaquila, A., Le Bao, C., Letourneur, D., & Simon‐Yarza, T. (2021) · Advanced Science · doi:10.1002/advs.202100798 · Cited by 115 · see citing works
In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed
Bonanini, F., Kurek, D., Previdi, S., Nicolas, A., Hendriks, D., de Ruiter, S., Meyer, M., Clapés Cabrer, M., Dinkelberg, R., García, S. B., et al. (2022) · Angiogenesis · doi:10.1007/s10456-022-09842-9 · Cited by 112 · see citing works
Microfluidic Tissue Engineering and Bio‐Actuation
Filippi, M., Buchner, T., Yaşa, Ö., Weirich, S., & Katzschmann, R. K. (2022) · Advanced Materials · doi:10.1002/adma.202108427 · Cited by 111 · see citing works
Velazquez, J. J., LeGraw, R., Moghadam, F., Tan, Y., Kilbourne, J. A., Maggiore, J. C., Hislop, J., Liu, S., Cats, D., Lopes, S. M. C. D. S., et al. (2020) · Cell Systems · doi:10.1016/j.cels.2020.11.002 · Cited by 103 · see citing works
El-Sadek, I. A., Miyazawa, A., Shen, L. T., Makita, S., Fukuda, S., Yamashita, T., Oka, Y., Mukherjee, P., Matsusaka, S., Oshika, T., et al. (2020) · Biomedical Optics Express · doi:10.1364/boe.404336 · Cited by 104 · see citing works
Barák, M., Fedorová, V., Pospíšilová, V., Raška, J., Vochyánová, S., Sedmík, J., Hříbková, H., Klímová, H., Váňová, T., & Bohačiaková, D. (2022) · Stem Cell Reviews and Reports · doi:10.1007/s12015-021-10254-3 · Cited by 100 · see citing works
Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategies
Naderi‐Meshkin, H., Cornelius, V. A., Eleftheriadou, M., Potel, K. N., Setyaningsih, W. A. W., & Margariti, A. (2023) · Stem Cell Research & Therapy · doi:10.1186/s13287-023-03521-2 · Cited by 99 · see citing works
Evolution of the Experimental Models of Cholangiocarcinoma
Massa, A., Varamo, C., Vita, F., Tavolari, S., Neia, C. P., Brandi, G., Rizzo, A., Cavalloni, G., & Aglietta, M. (2020) · Cancers · doi:10.3390/cancers12082308 · Cited by 95 · see citing works
Kim, S. K., Kim, Y. H., Park, S., & Cho, S. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.03.002 · Cited by 92 · see citing works
Role of three-dimensional cell culture in therapeutics and diagnostics: an updated review
Biju, T. S., Priya, V. V., & Francis, A. P. (2023) · Drug Delivery and Translational Research · doi:10.1007/s13346-023-01327-6 · Cited by 98 · see citing works
Bertassoni, L. E. (2021) · Advanced Materials · doi:10.1002/adma.202101321 · Cited by 96 · see citing works
Patient-derived cell line, xenograft and organoid models in lung cancer therapy
Huo, K.-G., D’Arcangelo, E., & Tsao, M. (2020) · Translational Lung Cancer Research · doi:10.21037/tlcr-20-154 · Cited by 93 · see citing works
Synthetic scaffolds for 3D cell cultures and organoids: applications in regenerative medicine
Marchini, A., & Gelain, F. (2022) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1932716 · Cited by 91 · see citing works
Sex differences in the blood–brain barrier and neurodegenerative diseases
Weber, C. M., & Clyne, A. M. (2021) · APL Bioengineering · doi:10.1063/5.0035610 · Cited by 91 · see citing works
Past, Present, and Future of Brain Organoid Technology.
Koo, B., Choi, B., Park, H., & Yoon, K. (2019) · Europe PMC (PubMed Central) · doi:10.14348/molcells.2019.0162 · Cited by 90 · see citing works
Peking, P., Krisch, L. M., Wolf, M., Hochmann, S., Hoog, A., Vári, B., Muigg, K., Poupardin, R., Scharler, C., Schmidhuber, S. M., et al. (2021) · Theranostics · doi:10.7150/thno.59661 · Cited by 90 · see citing works
Zheng, F., Xiao, Y., Liu, H., Fan, Y., & Dao, M. (2021) · Advanced Biology · doi:10.1002/adbi.202000024 · Cited by 92 · see citing works
Strategies for re-vascularization and promotion of angiogenesis in trauma and disease
Gonçalves, R. C., Banfi, A., Oliveira, M. B., & Mano, J. F. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120628 · Cited by 89 · see citing works
iPSC-derived organ-on-a-chip models for personalized human genetics and pharmacogenomics studies
Palasantzas, V. E. J. M., Tamargo-Rubio, I., Le, K. T. T., Slager, J., Wijmenga, C., Jonkers, I. H., Kumar, V., Fu, J., & Withoff, S. (2023) · Trends in Genetics · doi:10.1016/j.tig.2023.01.002 · Cited by 88 · see citing works
3D tumor angiogenesis models: recent advances and challenges
Bhat, S. M., Badiger, V. A., Vasishta, S., Chakraborty, J., Seetharam, P., Ghosh, S., & Joshi, M. B. (2021) · Journal of Cancer Research and Clinical Oncology · doi:10.1007/s00432-021-03814-0 · Cited by 86 · see citing works
Organoid Models for Precision Cancer Immunotherapy
Sun, C., Lan, H., Fang, X., Yang, X., & Jin, K. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.770465 · Cited by 87 · see citing works
The endothelium, a key actor in organ development and hPSC-derived organoid vascularization
Vargas-Valderrama, A., Messina, A., Mitjavila‐Garcia, M. T., & Guénou, H. (2020) · Journal of Biomedical Science · doi:10.1186/s12929-020-00661-y · Cited by 86 · see citing works
Chaudhary, S., & Chakraborty, E. (2022) · Beni-Suef University Journal of Basic and Applied Sciences · doi:10.1186/s43088-021-00172-1 · Cited by 79 · see citing works
Bioengineered skin organoids: from development to applications
Hong, Z.-X., Zhu, S.-T., Li, H., Luo, J.-Z., Yang, Y., An, Y., Wang, X., & Wang, K. (2023) · Military Medical Research · doi:10.1186/s40779-023-00475-7 · Cited by 77 · see citing works
Hair follicle stem cells as a skin‐organizing signaling center during adult homeostasis
Li, K. N., & Tumbar, T. (2021) · The EMBO Journal · doi:10.15252/embj.2020107135 · Cited by 75 · see citing works
Advances in development and application of human organoids
Shankaran, A., Prasad, K., Chaudhari, S., Brand, A. M., & Satyamoorthy, K. (2021) · 3 Biotech · doi:10.1007/s13205-021-02815-7 · Cited by 74 · see citing works
Organoid Technology: Current Standing and Future Perspectives
Shariati, L., Esmaeili, Y., Javanmard, S. H., Bidram, E., & Amini, A. (2021) · Stem Cells · doi:10.1002/stem.3379 · Cited by 69 · see citing works
Preclinical Evaluation of CAR T Cell Function: In Vitro and In Vivo Models
Si, X., Xiao, L., Brown, C. E., & Wang, D. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23063154 · Cited by 69 · see citing works
Geometric engineering of organoid culture for enhanced organogenesis in a dish
Park, S. E., Kang, S., Paek, J., Georgescu, A., Chang, J., Yi, A. Y., Wilkins, B. J., Karakasheva, T. A., Hamilton, K. E., & Huh, D. (2022) · Nature Methods · doi:10.1038/s41592-022-01643-8 · Cited by 66 · see citing works
Singh, R. K., & Nasonkin, I. O. (2020) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2020.00179 · Cited by 66 · see citing works
Natural Hydrogels Support Kidney Organoid Generation and Promote In Vitro Angiogenesis
Garreta, E., Moya‐Rull, D., Marco, A., Amato, G., Ullate‐Agote, A., Tarantino, C., Gallo, M., Esporrín‐Ubieto, D., Centeno, A., Vilas‐Zornoza, A., et al. (2024) · Advanced Materials · doi:10.1002/adma.202400306 · Cited by 63 · see citing works
Teng, Y., Zhao, Z., Tasnim, F., Huang, X., & Yu, H. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.120904 · Cited by 62 · see citing works
Advances in Microfluidic Technologies in Organoid Research
Liu, H., Gan, Z., Qin, X., Wang, Y., & Qin, J. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202302686 · Cited by 64 · see citing works
Kim, D., Lim, H., Youn, J., Park, T., & Kim, D. S. (2024) · Nature Communications · doi:10.1038/s41467-024-53073-z · Cited by 65 · see citing works
Establishment of Colorectal Cancer Organoids in Microfluidic-Based System
Pinho, D., Santos, D. R. D., Vila, A., & Carvalho, S. (2021) · Micromachines · doi:10.3390/mi12050497 · Cited by 59 · see citing works
Biohybrid Actuators for Soft Robotics: Challenges in Scaling Up
Won, P., Ko, S. H., Majidi, C., Feinberg, A. W., & Webster‐Wood, V. A. (2020) · Actuators · doi:10.3390/act9040096 · Cited by 60 · see citing works
Pinton, L., Khedr, M., Lionello, V. M., Sarcar, S., Maffioletti, S. M., Dastidar, S., Négroni, E., Choi, S., Khokhar, N., Bigot, A., et al. (2023) · Nature Protocols · doi:10.1038/s41596-022-00790-8 · Cited by 59 · see citing works
Moor, L. D., Smet, J., Plovyt, M., Bekaert, B., Vercruysse, C., Asadian, M., Geyter, N. D., Vlierberghe, S. V., Dubruel, P., & Declercq, H. (2021) · Biofabrication · doi:10.1088/1758-5090/ac24de · Cited by 59 · see citing works
de Jongh, R., Spijkers, X. M., Pasteuning-Vuhman, S., Vulto, P., & Pasterkamp, R. J. (2020) · Journal of Neurochemistry · doi:10.1111/jnc.15289 · Cited by 58 · see citing works
Cassotta, M., Forbes-Hernández, T. Y., Iglesias, R. C., Ruíz, R., Elexpuru-Zabaleta, M., Giampieri, F., & Battino, M. A. (2020) · Nutrients · doi:10.3390/nu12061827 · Cited by 57 · see citing works
Advanced lung organoids for respiratory system and pulmonary disease modeling
Joo, H., Min, S., & Cho, S. (2024) · Journal of Tissue Engineering · doi:10.1177/20417314241232502 · Cited by 60 · see citing works
Yan, L., Moriarty, R. A., & Stroka, K. M. (2021) · Theranostics · doi:10.7150/thno.63195 · Cited by 56 · see citing works
Cancer organoids: A platform in basic and translational research
Ma, X., Wang, Q. L., Li, G., Li, H., Xu, S., & Pang, D. (2023) · Genes & Diseases · doi:10.1016/j.gendis.2023.02.052 · Cited by 60 · see citing works
Bioengineering in vitro models of embryonic development
Gupta, A., Lütolf, M. P., Hughes, A. J., & Sonnen, K. F. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.04.005 · Cited by 53 · see citing works
An Air Bubble-Isolating Rotating Wall Vessel Bioreactor for Improved Spheroid/Organoid Formation
Phelan, M. A., Gianforcaro, A. L., Gerstenhaber, J. A., & Lelkes, P. I. (2019) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2019.0088 · Cited by 55 · see citing works
Bodnar, B. H., Zhang, Y., Liu, J., Lin, Y., Wang, P., Wei, Z., Saribas, S., Zhu, Y., Li, F., Wang, X., et al. (2021) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2021.682272 · Cited by 56 · see citing works
Engineered organoids for biomedical applications
de Barros, N. R., Wang, C., Maity, S., Peirsman, A., Nasiri, R., Herland, A., Ermis, M., Kawakita, S., de Carvalho, B. G., Kouchehbaghi, N. H., et al. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.115142 · Cited by 54 · see citing works
Bhattacharya, A., Alam, K., Roy, N. S., Kaur, K., Kaity, S., Ravichandiran, V., & Roy, S. (2023) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-023-02926-4 · Cited by 59 · see citing works
Generation of Skin Organoids: Potential Opportunities and Challenges
Sun, H., Zhang, Y., & Li, Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.709824 · Cited by 52 · see citing works
Vascularized human brain organoid on-chip.
Tan, S.-Y., Feng, X.-H., Cheng, L. K. W., & Wu, A. (2023) · Lab on a Chip · doi:10.1039/d2lc01109c · Cited by 49 · see citing works
What can biofabrication do for space and what can space do for biofabrication?
Moroni, L., Tabury, K., Stenuit, H., Grimm, D. G., Baatout, S., & Mironov, V. A. (2021) · Trends in biotechnology · doi:10.1016/j.tibtech.2021.08.008 · Cited by 52 · see citing works
Acharya, P., Choi, N.-Y., Shrestha, S., Jeong, S., & Lee, M.-Y. (2023) · Biotechnology and Bioengineering · doi:10.1002/bit.28606 · Cited by 38 · see citing works
Engineering the 3D structure of organoids
Moss, S. P., Bakırcı, E., & Feinberg, A. W. (2024) · Stem Cell Reports · doi:10.1016/j.stemcr.2024.11.009 · Cited by 51 · see citing works
Sound innovations for biofabrication and tissue engineering
Wu, M., Ma, Z., Tian, Z., Rich, J. T., He, X. B., Xia, J., He, Y., Yang, K., Yang, S., Leong, K. W., et al. (2024) · Microsystems & Nanoengineering · doi:10.1038/s41378-024-00759-5 · Cited by 48 · see citing works
Rajkumar, A. S., & Morrissey, J. P. (2020) · Microbial Cell Factories · doi:10.1186/s12934-020-01461-7 · Cited by 48 · see citing works
Human iPSC-Based Modeling of Central Nerve System Disorders for Drug Discovery
Lu, Q., & Julia, T. C. W. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22031203 · Cited by 48 · see citing works
Organoid co‐culture models of the tumor microenvironment promote precision medicine
Gu, Z., Wu, Q., Shang, B., Zhang, K., & Zhang, W. (2024) · Cancer Innovation · doi:10.1002/cai2.101 · Cited by 47 · see citing works
A human induced pluripotent stem cell-based modular platform to challenge sensorineural hearing loss
Zine, A., Messat, Y., & Fritzsch, B. (2021) · Stem Cells · doi:10.1002/stem.3346 · Cited by 46 · see citing works
Toward the next generation of vascularized human neural organoids
Li, M., Gao, L., Zhao, L., Zou, T., & Xu, H. (2022) · Medicinal Research Reviews · doi:10.1002/med.21922 · Cited by 44 · see citing works
Wang, J., Chen, C., Wang, L., Xie, M., Ge, X., Wu, S., He, Y., Mou, X., Ye, C., & Sun, Y. (2022) · Frontiers in Oncology · doi:10.3389/fonc.2022.872531 · Cited by 44 · see citing works
Artificial Muscles and Soft Robotic Devices for Treatment of End‐Stage Heart Failure
Weymann, A., Foroughi, J., Vardanyan, R., Punjabi, P. P., Schmack, B., Aloko, S., Spinks, G. M., Wang, C., Rad, A. A., & Ruhparwar, A. (2022) · Advanced Materials · doi:10.1002/adma.202207390 · Cited by 44 · see citing works
Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease
Gopal, S., Rodrigues, A. L., & Dordick, J. S. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00692 · Cited by 44 · see citing works
Mild formation of core–shell hydrogel microcapsules for cell encapsulation
Liu, Z., Zhang, H., Zhan, Z., Nan, H., Huang, N., Xu, T., Gong, X., & Hu, C. (2020) · Biofabrication · doi:10.1088/1758-5090/abd076 · Cited by 41 · see citing works
Fang, Y., Sun, W., Zhang, T., & Xiong, Z. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2021.121298 · Cited by 40 · see citing works
hPSC-derived organoids: models of human development and disease
Frum, T., & Spence, J. R. (2020) · Journal of Molecular Medicine · doi:10.1007/s00109-020-01969-w · Cited by 40 · see citing works
Panoutsopoulos, A. A. (2020) · The Neuroscientist · doi:10.1177/1073858420960112 · Cited by 39 · see citing works
Vivarelli, S., Candido, S., Caruso, G., Falzone, L., & Libra, M. (2020) · Cancers · doi:10.3390/cancers12123636 · Cited by 41 · see citing works
Human neural organoids: Models for developmental neurobiology and disease
Guy, B., Zhang, J. S., Duncan, L. H., & Johnston, R. J. (2021) · Developmental Biology · doi:10.1016/j.ydbio.2021.06.012 · Cited by 41 · see citing works
Organoid transplant approaches for the liver
Reza, H. A., Okabe, R., & Takebe, T. (2021) · Transplant International · doi:10.1111/tri.14128 · Cited by 39 · see citing works
Patient‐Derived Microphysiological Systems for Precision Medicine
Ko, J., Song, J., Choi, N., & Kim, H. N. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202303161 · Cited by 40 · see citing works
Shaping the Neurovascular Unit Exploiting Human Brain Organoids
Rizzuti, M., Melzi, V., Brambilla, L., Quetti, L., Sali, L., Ottoboni, L., Meneri, M., Ratti, A., Verde, F., Ticozzi, N., et al. (2024) · Molecular Neurobiology · doi:10.1007/s12035-024-03998-9 · Cited by 38 · see citing works
Designer cellular spheroids with DNA origami for drug screening
Wei, J., Sun, Y., Wang, H., Zhu, T., Li, L., Zhou, Y., Liu, Q., Dai, Z., Li, W., Yang, T., et al. (2024) · Science Advances · doi:10.1126/sciadv.ado9880 · Cited by 40 · see citing works
Mesenchymal Stem Cells as a Promising Cell Source for Integration in Novel In Vitro Models
Afflerbach, A., Kiri, M. D., Zur, T. D., & Maoz, B. M. (2020) · Biomolecules · doi:10.3390/biom10091306 · Cited by 39 · see citing works
Precision-based modeling approaches for necrotizing enterocolitis
Kovler, M. L., Sodhi, C. P., & Hackam, D. J. (2020) · Disease Models & Mechanisms · doi:10.1242/dmm.044388 · Cited by 37 · see citing works
Jacquet, A. D. R., Denis, H. L., Cicchetti, F., & Alpaugh, M. (2021) · Molecular Psychiatry · doi:10.1038/s41380-020-00999-7 · Cited by 35 · see citing works
Engineering a Vascularized 3D Hybrid System to Model Tumor-Stroma Interactions in Breast Cancer
Teixeira, F. C., Chaves, S., Torres, A. L., Barrias, C. C., & Bidarra, S. J. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.647031 · Cited by 36 · see citing works
Torrens‐Mas, M., Perelló-Reus, C. M., Navas-Enamorado, C., Ibargüen-González, L., Sánchez-Polo, A., Segura‐Sampedro, J. J., Masmiquel, L., Barceló, C., & González‐Freire, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms221910547 · Cited by 36 · see citing works
Tricot, T., Verfaillie, C. M., & Kumar, M. (2022) · Cells · doi:10.3390/cells11030442 · Cited by 35 · see citing works
Advances in the Model Structure of In Vitro Vascularized Organ-on-a-Chip
Yin, H., Wang, Y., Liu, N., Zhong, S., Li, L., Zhang, Q., Liu, Z., & Yue, T. (2024) · Cyborg and Bionic Systems · doi:10.34133/cbsystems.0107 · Cited by 36 · see citing works
Reverse engineering human brain evolution using organoid models
Mostajo-Radji, M. A., Schmitz, M. T., Torres, S., & Pollen, A. A. (2019) · Brain Research · doi:10.1016/j.brainres.2019.146582 · Cited by 34 · see citing works
Sharma, S., Venzac, B., Burgers, T. C. Q., Gac, S. L., & Schlatt, S. (2020) · Molecular Human Reproduction · doi:10.1093/molehr/gaaa006 · Cited by 34 · see citing works
- Authors
- Grebenyuk, S., & Ranga, A.
- Affiliations
- KU Leuven (BE)
- Corresponding author
- Ranga, Adrian
- Venue
- Frontiers in Bioengineering and Biotechnology
- Publisher
- Frontiers Media SA
- Published
- 2019-03-19
- Volume
- 7
- Pages
- 39-39
- ISSN
- 2296-4185 (ISSN-L)
- PMC
- PMC6433749
- OpenAlex
- W2923099616
- Cited by
- 295
- References
- 80
- Citations by year
- 2026: 27 · 2025: 25 · 2024: 35 · 2023: 47 · 2022: 42 · 2021: 73 · 2020: 38 · 2019: 8
- FWCI
- 3.24
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Lirias (KU Leuven) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Fonds Wetenschappelijk Onderzoek (G087018N, C14/17/111)
- KU Leuven (C32/17/027, C14/17/111)
- Vlaamse regering
- Interreg
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Keywords
- organoids, organoid vascularization, vascular system, tissue engineering, organoid development, tissue organization, drug discovery, regenerative medicine
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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