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Acute Energy Deprivation Affects Skeletal Muscle Protein Synthesis and Associated Intracellular Signaling Proteins in Physically Active Adults
Open accessAbstract
To date, few studies have characterized the influence of energy deprivation on direct measures of skeletal muscle protein turnover. In this investigation, we characterized the effect of an acute, moderate energy deficit (10 d) on mixed muscle fractional synthetic rate (FSR) and associated intracellular signaling proteins in physically active adults. Eight men and 4 women participated in a 20-d, 2-phase diet intervention study: weight maintenance (WM) and energy deficient (ED; approximately 80% of estimated energy requirements). Dietary protein (1.5 g x kg(-1) x d(-1)) and fat (approximately 30% of total energy) were constant for WM and ED. FSR and intracellular signaling proteins were measured on d 10 of both interventions using a primed, constant infusion of [(2)H(5)]-phenylalanine and Western blotting techniques, respectively. Participants lost approximately 1 kg body weight during ED (P < 0.0001). FSR was reduced approximately 19% (P < 0.05) for ED (0.06 +/- 0.01%/h) compared with WM (0.074 +/- 0.01%/h). Protein kinase B and eukaryotic initiation factor 4E binding protein 1 phosphorylation were lower (P < 0.05) during ED compared with WM. AMP activated protein kinase phosphorylation decreased (P < 0.05) over time regardless of energy status. These findings show that FSR and associated synthetic intracellular signaling proteins are downregulated in response to an acute, moderate energy deficit in physically active adults and provide a basis for future studies assessing the impact of prolonged, and perhaps more severe, energy restriction on skeletal muscle protein turnover.
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“there is good physiological reason to expect it to be constrained under caloric deficit [163]”
From Ribosome Biogenesis as a Putative Bottleneck to Skeletal Muscle Hypertrophy: Mechanisms, Human Evidence, and Practical Modulators (2026) · cites this paper as [163]
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“Energy availability may be even more important than total protein intake in determining whether ribosome biogenesis can be supported over time.”
From Ribosome Biogenesis as a Putative Bottleneck to Skeletal Muscle Hypertrophy: Mechanisms, Human Evidence, and Practical Modulators (2026) · cites this paper as [163]
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“this provides a mechanism whereby enhanced SM proteolysis occurs in response to hypoinsulinaemia (a physiological feature of NEB) [85,90].”
From Optimised Skeletal Muscle Mass as a Key Strategy for Obesity Management (2025) · cites this paper as [90]
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“a 10-day moderate NEB resulted in a 19% reduction in fasting SM protein synthesis [85,90].”
From Optimised Skeletal Muscle Mass as a Key Strategy for Obesity Management (2025) · cites this paper as [90]
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“Pasiakos et al. [85] reported a 19% decrease in MPS during 10 days of moderate caloric deficit (20% below maintenance).”
From International Society of Sports Nutrition position stand: diets for body weight reduction (2026) · cites this paper as [85]
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“Current evidence suggests that the energy deficit likely reduces basal MPS (29) and may also have reduced the sensitivity of MPS to feeding (30,31).”
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“Ribosome production is metabolically expensive”
From Ribosome Biogenesis as a Putative Bottleneck to Skeletal Muscle Hypertrophy: Mechanisms, Human Evidence, and Practical Modulators (2026) · cites this paper as [163]
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Cites
Abràmoff, M. D., Magalhães, P. J., & Ram, S. J. (2004) · Utrecht University Repository (Utrecht University) · Cited by 11,816
Compendium of physical activities: an update of activity codes and MET intensities
AINSWORTH, B. E., HASKELL, W. L., WHITT, M. C., IRWIN, M. L., SWARTZ, A. M., STRATH, S. J., O???BRIEN, W. L., BASSETT, D. R., SCHMITZ, K. H., EMPLAINCOURT, P. O., et al. (2000) · Medicine & Science in Sports & Exercise · doi:10.1097/00005768-200009001-00009 · Cited by 8,497
Reduced Rate of Energy Expenditure as a Risk Factor for Body-Weight Gain
Ravussin, E., Lillioja, S., Knowler, W. C., Christin, L., Freymond, D., Abbott, W. G. H., Boyce, V., Howard, B. V., & Bogardus, C. (1988) · New England Journal of Medicine · doi:10.1056/nejm198802253180802 · Cited by 1,215
Mixed muscle protein synthesis and breakdown after resistance exercise in humans
Phillips, S. M., Tipton, K. D., Aarsland, A., Wolf, S. E., & Wolfe, R. R. (1997) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1997.273.1.e99 · Cited by 1,132
Bolster, D. R., Crozier, S. J., Kimball, S. R., & Jefferson, L. S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c200171200 · Cited by 851
Volpi, E., Kobayashi, H., Sheffield-Moore, M., Mittendorfer, B., & Wolfe, R. R. (2003) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/78.2.250 · Cited by 846
An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein
Biolo, G., Tipton, K. D., Klein, S., & Wolfe, R. R. (1997) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1997.273.1.e122 · Cited by 825
Kimball, S. R., & Jefferson, L. S. (2006) · Journal of Nutrition · doi:10.1093/jn/136.1.227s · Cited by 637
Dreyer, H. C., Fujita, S., Cadenas, J. G., Chinkes, D. L., Volpi, E., & Rasmussen, B. B. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2006.113175 · Cited by 570
Layman, D. K., Boileau, R. A., Erickson, D. J., Painter, J. E., Shiue, H., Sather, C., & Christou, D. D. (2003) · The Journal of Nutrition · doi:10.1093/jn/133.2.411 · Cited by 541
hVps34 Is a Nutrient-regulated Lipid Kinase Required for Activation of p70 S6 Kinase
Byfield, M. P., Murray, J. T., & Backer, J. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m507201200 · Cited by 540
Bohé, J., Low, A., Wolfe, R. R., & Rennie, M. J. (2003) · The Journal of Physiology · doi:10.1113/jphysiol.2003.050674 · Cited by 496
Greenhaff, P. L., Karagounis, L. G., Peirce, N., Simpson, E. J., Hazell, M., Layfield, R., Wackerhage, H., Smith, K., Atherton, P., Selby, A., et al. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90411.2008 · Cited by 495
Noakes, M., Keogh, J. B., Foster, P. R., & Clifton, P. M. (2005) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/81.6.1298 · Cited by 483
Amino acid ingestion improves muscle protein synthesis in the young and elderly
Paddon-Jones, D., Sheffield-Moore, M., Zhang, X.-J., Volpi, E., Wolf, S. E., Aarsland, A., Ferrando, A. A., & Wolfe, R. R. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00368.2003 · Cited by 467
Exogenous amino acids stimulate net muscle protein synthesis in the elderly.
Volpi, E., Ferrando, A. A., Yeckel, C. W., Tipton, K. D., & Wolfe, R. R. (1998) · Journal of Clinical Investigation · doi:10.1172/jci939 · Cited by 390
Self-citationMillward, D. J., Layman, D. K., Tomé, D., & Schaafsma, G. (2008) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/87.5.1576s · Cited by 359
Nutrient signalling in the regulation of human muscle protein synthesis
Fujita, S., Dreyer, H. C., Drummond, M. J., Glynn, E. L., Cadenas, J. G., Yoshizawa, F., Volpi, E., & Rasmussen, B. B. (2007) · The Journal of Physiology · doi:10.1113/jphysiol.2007.134593 · Cited by 339
Drummond, M. J., Dreyer, H. C., Fry, C. S., Glynn, E. L., & Rasmussen, B. B. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.91397.2008 · Cited by 322
P ROTEIN AND A MINO A CID M ETABOLISM D URING AND A FTER E XERCISE AND THE E FFECTS OF N UTRITION
Rennie, M. J., & Tipton, K. D. (2000) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.20.1.457 · Cited by 321
Nutrient control of TORC1, a cell-cycle regulator
Wang, X., & Proud, C. G. (2009) · Trends in Cell Biology · doi:10.1016/j.tcb.2009.03.005 · Cited by 197
Jiang, W., Zhu, Z., & Thompson, H. J. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-6721 · Cited by 176
Dietary composition and physiologic adaptations to energy restriction
Agus, M. S. D., SWAIN, J. F., Larson, C. L., Eckert, E. A., & Ludwig, D. S. (2000) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/71.4.901 · Cited by 163
Leucine Regulates Translation Initiation in Rat Skeletal Muscle Via Enhanced eIF4G Phosphorylation
Bolster, D. R., Vary, T. C., Kimball, S. R., & Jefferson, L. S. (2004) · Journal of Nutrition · doi:10.1093/jn/134.7.1704 · Cited by 143
Nitrogen Balance of Men with Marginal Intakes of Protein and Energy
Calloway, D. H. (1975) · Journal of Nutrition · doi:10.1093/jn/105.7.914 · Cited by 138
Metabolic effects of very low calorie weight reduction diets.
Hoffer, L. J., Bistrian, B. R., Young, V. R., Blackburn, G. L., & Matthews, D. E. (1984) · Journal of Clinical Investigation · doi:10.1172/jci111268 · Cited by 135
Nitrogen Balance in Men with Adequate and Deficient Energy Intake at Three Levels of Work
Todd, K. S., Butterfield, G. E., & Calloway, D. H. (1984) · Journal of Nutrition · doi:10.1093/jn/114.11.2107 · Cited by 119
Bennet, W. M., Connacher, A. A., Scrimgeour, C. M., & Rennie, M. J. (1990) · European Journal of Clinical Investigation · doi:10.1111/j.1365-2362.1990.tb01789.x · Cited by 119
Leucine, glucose, and energy metabolism after 3 days of fasting in healthy human subjects
Nair, K. S., Woolf, P. D., Welle, S. L., & Matthews, D. E. (1987) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/46.4.557 · Cited by 119
The Recommended Dietary Allowance of Protein: A Misunderstood Concept
Wolfe, R. R., & Miller, S. L. (2008) · JAMA · doi:10.1001/jama.299.24.2891 · Cited by 107
Self-citationInfluence of low-energy diets on whole-body protein turnover in obese subjects
Garlick, P. J., Clugston, G. A., & Waterlow, J. C. (1980) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1980.238.3.e235 · Cited by 102
Heated dorsal hand vein sampling for metabolic studies: a reappraisal
Copeland, K. C., Kenney, F. A., & Nair, K. S. (1992) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1992.263.5.e1010 · Cited by 97
Effect of Chronic Endurance Exercise on Retention of Dietary Protein
Friedman, J., & Lemon, P. (1989) · International Journal of Sports Medicine · doi:10.1055/s-2007-1024886 · Cited by 95 · cited 1×
“Apparent nitrogen balance was calculated as the difference of nitrogen intake minus urinary nitrogen excretion plus estimated integumental losses (18).”
BackgroundBasal muscle intracellular amino acid kinetics in women and men
Fujita, S., Rasmussen, B. B., Bell, J. A., Cadenas, J. G., & Volpi, E. (2006) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00173.2006 · Cited by 92
Bolster, D. R., Pikosky, M. A., Gaine, P. C., Martin, W. F., Wolfe, R. R., Tipton, K. D., Maclean, D., Maresh, C. M., & Rodriguez, N. R. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00060.2005 · Cited by 75
Leucine metabolism during fasting and exercise
Knapik, J. J., Meredith, C. N., Jones, B., Fielding, R. A., Young, V. R., & Evans, W. P. (1991) · Journal of Applied Physiology · doi:10.1152/jappl.1991.70.1.43 · Cited by 74
Effect of reduced dietary intake on energy expenditure, protein turnover, and glucose cycling in man
Stein, T. P., Rumpler, W. V., Leskiw, M. J., Schluter, M. D., Staples, R., & Bodwell, C. E. (1991) · Metabolism · doi:10.1016/0026-0495(91)90228-o · Cited by 64
Response of alanine metabolism in humans to manipulation of dietary protein and energy intakes
Yang, R. D., Matthews, D. E., Bier, D. M., Wen, Z. M., & Young, V. R. (1986) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1986.250.1.e39 · Cited by 57
Three weeks of caloric restriction alters protein metabolism in normal-weight, young men
Friedlander, A. L., Braun, B., Pollack, M., MacDonald, J. R., Fulco, C. S., Muza, S. R., Rock, P. B., Henderson, G. C., Horning, M. A., Brooks, G. A., et al. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00001.2005 · Cited by 56
Increased Protein Maintains Nitrogen Balance during Exercise-Induced Energy Deficit
Pikosky, M. A., Smith, T. J., Grediagin, A., Castaneda‐Sceppa, C., Byerley, L. O., Glickman, E. L., & Young, A. (2008) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e31815f6643 · Cited by 52
Increased leucine flux in short-term fasted human subjects: evidence for increased proteolysis
Tsalikian, E., Howard, C., Gerich, J. E., & Haymond, M. W. (1984) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1984.247.3.e323 · Cited by 52
Protein metabolic effects of a prolonged fast and hypocaloric refeeding
Hoffer, L. J., & Forse, R. A. (1990) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1990.258.5.e832 · Cited by 52
VO2max during horizontal and inclined treadmill running
Kasch, F. W., Wallace, J. P., Huhn, R. R., Krogh, L. A., & Hurl, P. M. (1976) · Journal of Applied Physiology · doi:10.1152/jappl.1976.40.6.982 · Cited by 38
Introduction to Protein Summit 2007: Exploring the Impact of High-Quality Protein on Optimal Health
Rodriguez, N. R., & Garlick, P. J. (2008) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/87.5.1551s · Cited by 36
Jiang, W., Zhu, Z., & Thompson, H. J. (2008) · Molecular Carcinogenesis · doi:10.1002/mc.20425 · Cited by 32
Protein and Energy Interactions Throughout Life1
Young, V. R., Yu, Y., & Fukagawa, N. K. (1991) · Acta Paediatrica · doi:10.1111/j.1651-2227.1991.tb18147.x · Cited by 27
Protein metabolism in obese subjects during a very-low-energy diet
Gougeon, R., Hoffer, L. J., Pencharz, P. B., & Eb, M. (1992) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/56.1.249s · Cited by 17
Pasiakos, S. M., Mettel, J. B., West, K. L., Lofgren, I. E., Fernández, M. L., Koo, S. I., & Rodriguez, N. R. (2008) · Metabolism · doi:10.1016/j.metabol.2007.11.006 · Cited by 14
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Biolo, G., Cederholm, T., & Muscaritoli, M. (2014) · Clinical Nutrition · doi:10.1016/j.clnu.2014.03.007 · Cited by 418
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Devries, M. C., & Phillips, S. M. (2015) · Journal of Food Science · doi:10.1111/1750-3841.12802 · Cited by 355
Pasiakos, S. M., Cao, J. J., Margolis, L. M., Sauter, E. R., Whigham, L. D., McClung, J. P., Rood, J. C., Carbone, J. W., Combs, G. F., & Young, A. J. (2013) · The FASEB Journal · doi:10.1096/fj.13-230227 · Cited by 312
Self-citationInternational society of sports nutrition position stand: diets and body composition
Aragon, A. A., Schoenfeld, B. J., Wildman, R., Kleiner, S., VanDusseldorp, T., Taylor, L., Earnest, C. P., Arciero, P. J., Wilborn, C., Kalman, D. S., et al. (2017) · Journal of the International Society of Sports Nutrition · doi:10.1186/s12970-017-0174-y · Cited by 301
Metadata
- Authors
- Pasiakos, S. M., Vislocky, L. M., Carbone, J. W., Altieri, N., Konopelski, K., Freake, H. C., Anderson, J. M., Ferrando, A. A., Wolfe, R. R., & Rodriguez, N. R.
- Affiliations
- University of Connecticut (US)
- University of Arkansas for Medical Sciences (US)
- Corresponding author
- Rodriguez, Nancy R.
- Venue
- The Journal of Nutrition
- Publisher
- Elsevier BV
- Published
- 2010-04
- Volume
- 140
- Issue
- 4
- Pages
- 745-751
- ISSN
- 0022-3166 (ISSN-L)
- OpenAlex
- W2141308679
- Cited by
- 176
- References
- 48
- Citations by year
- 2026: 11 · 2025: 13 · 2024: 12 · 2023: 14 · 2022: 13 · 2021: 9 · 2020: 16 · 2019: 8 · 2018: 11 · 2017: 9 · 2016: 9 · 2015: 10 · 2014: 15 · 2013: 12 · 2012: 8
- FWCI
- 3.49
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Connecticut
- National Cattlemen's Beef Association
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology (medical) › Nutrition and Health in Aging
- Keywords
- caloric restriction, skeletal muscle protein synthesis, fractional synthesis rate, protein turnover, Akt, eIF4E-binding protein 1, AMPK, physically active adults, weight loss, dietary protein
- MeSH terms
- Diet, Energy Metabolism, Female, Gene Expression Regulation, Hormones, Humans, Male, Motor Activity, Muscle Proteins, Phenylalanine, Down-Regulation, Muscle, Skeletal, Caloric Restriction, Intracellular Signaling Peptides and Proteins, Amino-Acid N-Acetyltransferase, Young Adult
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