Cited by 37Gut microbiota and healthOral microbiology and periodontitis researchProbiotics and Fermented Foods

Metagenomic Analysis of the Gut Microbiome Reveals Enrichment of Menaquinones (Vitamin K2) Pathway in Diabetes Mellitus

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Dash, Nihar Ranjan, Al Bataineh, Mohammad Tahseen·2021·Diabetes & Metabolism Journal

Abstract

BACKGROUND: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease with a high prevalence worldwide, especially among overweight and obese populations. T2DM is multifactorial with several genetic and acquired risk factors that lead to insulin resistance. Mounting evidence indicates that alteration of gut microbiome composition contribute to insulin resistance and inflammation. However, the precise link between T2DM and gut microbiome role and composition remains unknown. METHODS: We evaluated the metabolic capabilities of the gut microbiome of twelve T2DM and six healthy individuals through shotgun metagenomics using MiSeq platform. RESULTS: We identified no significant differences in the overall taxonomic composition between healthy and T2DM subjects when controlling for differences in diet. However, results showed that T2DM enriched in metabolic pathways involved in menaquinone (vitamin K2) superpathway biosynthesis (PWY-5838) as compared to healthy individuals. Covariance analysis between the bacterial genera and metabolic pathways displaying difference in abundance (analysis of variance P<0.05) in T2DM as compared to healthy subjects revealed that genera belonging Firmicutes, Actinobacteria, and Bacteroidetes phyla contribute significantly to vitamin K2 biosynthesis. Further, the microbiome corresponding to T2DM with high glycosylated hemoglobin (HbA1c) (>6.5%) exhibit high abundance of genes involved in lysine biosynthesis and low abundance of genes involved in creatinine degradation as compared to T2DM with lower HbA1c (<6.5%). CONCLUSION: The identified differences in metabolic capabilities provide important information that may eventually lead to the development of novel biomarkers and more effective management strategies to treat T2DM.

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Authors
Dash, N. R., & Al Bataineh, M. T.
Affiliations
University of Sharjah (AE)
Venue
Diabetes & Metabolism Journal
Published
2021-01-31
Volume
45
Issue
1
Pages
77-85
ISSN
  • 2233-6079 (ISSN-L)
  • 2233-6087
OpenAlex
W3024695169
Cited by
37
References
44
Citations by year
2026: 5 · 2025: 5 · 2024: 6 · 2023: 8 · 2022: 10 · 2021: 3
FWCI
1.70
Type
journal article
Language
en
Open access
Locations
  • Diabetes & Metabolism Journal (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • University of Sharjah (P1701090226)
  • Boehringer Ingelheim (2016-17)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
type 2 diabetes, gut microbiota, menaquinone biosynthesis, vitamin K2, metabolic pathways, insulin resistance, HbA1c, lysine biosynthesis, Firmicutes, actinobacteria, Bacteroidetes, biomarkers
MeSH terms
Gastrointestinal Microbiome, Diabetes Mellitus, Type 2, Humans, Vitamin K 2, Metagenome, Metagenomics

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