Protein Blend and Casein Supplementations before Inactive Phase Similarly Activate Mechanistic Target of Rapamycin Signaling in Rat Skeletal Muscle

Vieira, Tales Sambrano; Pinto, Ana P.; Batitucci, Gabriela; da Rocha, Alisson L.; Filho, Hugo T.; Gonçalves, Dawit A.; da Silva, Adelino Sanchez R.; de Freitas, Ellen Cristini

doi:10.4103/cjp.cjp_31_20

Cited by 3Muscle metabolism and nutritionMuscle Physiology and DisordersNutrition and Health in Aging

Protein Blend and Casein Supplementations before Inactive Phase Similarly Activate Mechanistic Target of Rapamycin Signaling in Rat Skeletal Muscle

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Vieira, Tales Sambrano, Pinto, Ana P., Batitucci, Gabriela, da Rocha, Alisson L., Filho, Hugo T., Gonçalves, Dawit A., da Silva, Adelino Sanchez R., de Freitas, Ellen Cristini·2020·Chinese Journal of Physiology

DOI 10.4103/cjp.cjp_31_20

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Abstract

During overnight sleep, the longest postabsorptive and inactive phase of the day causes protein catabolism and loss. However, the daytime ingestion of dairy proteins has been shown to stimulate muscle protein synthesis and growth. This study compared the effects of pre-sleep supplementation of a protein blend (PB) composed of micellar casein (MCa) and whey protein (1:1) versus isolate MCa on the plasma levels of branched-chain amino acids (BCAAs) and the activation of the mechanistic target of rapamycin (mTOR) signaling, a critical intracellular pathway involved in the regulation of muscle protein synthesis. After 10 h of fasting during the active phase, rats were fed with a single dose of PB or MCa (5.6 g protein/kg of body mass) by gavage, and samples of blood and gastrocnemius muscle were collected at 30, 90, and 450 min. PB and MCa supplementations induced an increase (~3-fold, P < 0.001) of plasma BCAAs at 30 and 90 min. Most importantly, the stimulatory phosphorylation levels of mTOR and its downstream target p70 ribosomal protein S6 kinase (p70S6K) were similarly higher (~2.5-fold, P < 0.001) 30 and 90 min after MCa and PB. Plasma levels of leucine, isoleucine, valine, and overall BCAAs were correlated with the activation of mTOR (P < 0.001) and p70S6K (P < 0.001). MCa and PB supplementations before the inactive phase of rats resulted in an anabolic milieu in the skeletal muscle by inducing a transient increase in plasma BCAAs and a similar activation of the mTOR/p70S6K axis.

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Before the inactive phase, rat micellar casein and whey protein (1:1) supplementation increases muscle protein synthesis due to increased plasma branched-chain amino acids and activation of downstream targets of mTOR signaling.
“Similarly, a study showed that before the inactive phase of rat micellar casein and whey protein (1:1) supplementation increase muscle protein synthesis due to increased plasma levels of branched-chain amino acids and the activation of the downstream targets of mTOR signaling [30].”
From Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy (2021) · cites this paper as [30]

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Effects of protein blend and micellar casin suplementations on mTOR signaling pathway in skeletal muscles of Wistar rats
Vieira, T. S. (2019) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)
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Protein blend ingestion following resistance exercise promotes human muscle protein synthesis
Reidy, P. T., Walker, D. K., Dickinson, J. M., Gundermann, D. M., Drummond, M. J., Timmerman, K. L., Fry, C. S., Borack, M. S., Cope, M. B., Mukherjea, R., et al. (2013) · The Journal of Nutrition · doi:10.3945/jn.112.168021 · Cited by 178 · see citing works
Branched-Chain Amino Acid Ingestion Stimulates Muscle Myofibrillar Protein Synthesis following Resistance Exercise in Humans
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Soy-dairy protein blend or whey protein isolate ingestion induces similar postexercise muscle mechanistic target of rapamycin complex 1 signaling and protein synthesis responses in older men
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Authors: Vieira, T. S., Pinto, A. P., Batitucci, G., da Rocha, A. L., Filho, H. T., Gonçalves, D. A., da Silva, A. S. R., & de Freitas, E. C.
Affiliations: Universidade Estadual Paulista (Unesp) (BR)
Universidade de São Paulo (BR)
Universidade Federal de Minas Gerais (BR)
ORCID: Vieira, Tales Sambrano
Pinto, Ana P.
Batitucci, Gabriela
Filho, Hugo T.
Gonçalves, Dawit A.
Venue: Chinese Journal of Physiology
Publisher: Ovid Technologies (Wolters Kluwer Health)
Published: 2020
Volume: 63
Issue: 4
Pages: 171-178
ISSN: 0304-4920 (ISSN-L)
DOI: 10.4103/cjp.cjp_31_20
OpenAlex: W3082129757
Cited by: 3
References: 38
Citations by year: 2024: 1 · 2023: 1 · 2021: 1
FWCI: 0.25
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by-nc-sa
Locations: The Chinese Journal of Physiology (publishedVersion, cc-by-nc-sa)
PubMed (publishedVersion)
LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
Keywords: protein supplementation, micellar casein, whey protein, mTOR signaling, p70 S6 kinase, branched-chain amino acids, skeletal muscle protein synthesis, sleep, leucine, gastrocnemius
MeSH terms: Animals, Caseins, Leucine, Phosphorylation, Muscle, Skeletal, Dietary Supplements, Sirolimus, Rats, TOR Serine-Threonine Kinases

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Protein Blend and Casein Supplementations before Inactive Phase Similarly Activate Mechanistic Target of Rapamycin Signaling in Rat Skeletal Muscle

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