Resistance Exercise Augments Postprandial Overnight Muscle Protein Synthesis Rates

TROMMELEN, JORN; HOLWERDA, ANDREW M.; KOUW, IMRE W. K.; LANGER, HENNING; HALSON, SHONA L.; ROLLO, IAN; VERDIJK, LEX B.; VAN LOON, LUC J. C.

doi:10.1249/mss.0000000000001045

Cited by 69Muscle metabolism and nutritionMuscle Physiology and DisordersCardiovascular and exercise physiology

Resistance Exercise Augments Postprandial Overnight Muscle Protein Synthesis Rates

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TROMMELEN, JORN, HOLWERDA, ANDREW M., KOUW, IMRE W. K., LANGER, HENNING, HALSON, SHONA L., ROLLO, IAN, VERDIJK, LEX B., VAN LOON, LUC J. C.·2016·Medicine & Science in Sports & Exercise

DOI 10.1249/mss.0000000000001045

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Abstract

INTRODUCTION: We have previously shown that protein ingestion before sleep increases overnight muscle protein synthesis rates. Whether prior exercise further augments the muscle protein synthetic response to presleep protein ingestion remains to be established. OBJECTIVE: This study aimed to assess whether resistance-type exercise performed in the evening increases the overnight muscle protein synthetic response to presleep protein ingestion. METHODS: Twenty-four healthy young men were randomly assigned to ingest 30 g intrinsically L-[1-C]-phenylalanine and L-[1-C]-leucine-labeled casein protein before going to sleep with (PRO + EX, n = 12) or without (PRO, n = 12) prior resistance-type exercise performed in the evening. Continuous intravenous L-[ring-H5]-phenylalanine, L-[1-C]-leucine, and L-[ring-H2]-tyrosine infusions were applied. Blood and muscle tissue samples were collected to assess whole-body protein balance, myofibrillar protein synthesis rates, and overnight incorporation of dietary protein-derived amino acids into de novo myofibrillar protein. RESULTS: A total of 57% ± 1% of the ingested protein-derived phenylalanine appeared in the circulation during overnight sleep. Overnight myofibrillar protein synthesis rates were 37% (0.055%·h ± 0.002%·h vs. 0.040%·h ± 0.003%·h, P < 0.001, based on L-[ring- H5]-phenylalanine) and 31% (0.073%·h ± 0.004%·h vs. 0.055%·h ± 0.006%·h, P = 0.024, based on L-[1-C]-leucine) higher in PRO + EX compared with PRO. Substantially more of the dietary protein-derived amino acids were incorporated into de novo myofibrillar protein during overnight sleep in PRO + EX compared with PRO (0.026 ± 0.003 vs. 0.015 ± 0.003 molar percent excess, P = 0.012). CONCLUSIONS: Resistance-type exercise performed in the evening augments the overnight muscle protein synthetic response to presleep protein ingestion and allows more of the ingested protein-derived amino acids to be used for de novo myofibrillar protein synthesis during overnight sleep.

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Exercise performed in the evening augments the overnight MPS response in both younger and older men.
“data supports that exercise performed in the evening augments the overnight MPS response in both younger and older men [119–121].”
From International Society of Sports Nutrition Position Stand: protein and exercise (2017) · cites this paper as [121]
In 24 young men ingesting 30 g of casein protein with or without completion of a single bout of resistance exercise, rates of MPS were increased, but whole-body protein synthesis rates were not impacted.
“Trommelen and investigators [121] examined 24 young men ingesting 30 g of casein protein with or without completion of a single bout of resistance exercise, and concluded that rates of MPS were increased, but whole-body protein synthesis rates were not impacted.”
From International Society of Sports Nutrition Position Stand: protein and exercise (2017) · cites this paper as [121]

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Resistance Exercise Augments Postprandial Overnight Muscle Protein Synthesis Rates [accepted manuscript]
Trommelen, J., Holwerda, A. M., Kouw, I. W. K., Langer, H., Halson, S. L., Verdijk, L. B., & van Loon, L. J. C. (2016) · Research Bank (Australian Catholic University)
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Exercise Enhances The Overnight Muscle Protein Synthetic Response To Pre-sleep Protein Feeding In Older Males
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Presleep protein ingestion does not compromise the muscle protein synthetic response to protein ingested the following morning [accepted manuscript]
Wall, B. T., Burd, N. A., Franssen, R., Gorissen, S. H. M., Snijders, T., Senden, J. M., Gijsen, A. P., & van Loon, L. J. C. (2016) · Research Bank (Australian Catholic University)
Presleep protein ingestion does not compromise the muscle protein synthetic response to protein ingested the following morning
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Protein Ingestion Before Sleep Provides Precursors For Post-exercise Overnight De Novo Muscle Protein Synthesis
Trommelen, J., Holwerda, A. M., Kouw, I. W. K., Halson, S. L., Verdijk, L. B., & van Loon, L. J. C. (2016) · Medicine & Science in Sports & Exercise · doi:10.1249/01.mss.0000485019.86027.3c
Self-citation

Authors: TROMMELEN, J., HOLWERDA, A. M., KOUW, I. W. K., LANGER, H., HALSON, S. L., ROLLO, I., VERDIJK, L. B., & VAN LOON, L. J. C.
Affiliations: Maastricht University Medical Centre (NL)
ORCID: TROMMELEN, JORN
HOLWERDA, ANDREW M.
KOUW, IMRE W. K.
LANGER, HENNING
HALSON, SHONA L.
ROLLO, IAN
VERDIJK, LEX B.
Venue: Medicine & Science in Sports & Exercise
Publisher: Ovid Technologies (Wolters Kluwer Health)
Published: 2016-12
Volume: 48
Issue: 12
Pages: 2517-2525
ISSN: 0195-9131 (ISSN-L)
DOI: 10.1249/mss.0000000000001045
OpenAlex: W2519362900
Cited by: 69
References: 32
Citations by year: 2025: 4 · 2024: 6 · 2023: 5 · 2022: 10 · 2021: 8 · 2020: 14 · 2019: 9 · 2018: 8 · 2017: 4 · 2016: 1
FWCI: 5.04
Type: journal article
Language: en
Open access: green
research.wur.nl
Locations: Medicine & Science in Sports & Exercise (publishedVersion)
PubMed (publishedVersion)
Research Publications (Maastricht University) (publishedVersion)
Socio-Environmental Systems Modeling (acceptedVersion)
Research Bank (Australian Catholic University) (submittedVersion)
VUBIR (Vrije Universiteit Brussel) (submittedVersion)
Research Publications (Maastricht University) (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Keywords: resistance training, myofibrillar protein synthesis, de novo protein synthesis, casein, protein balance, amino acid incorporation, muscle protein synthesis
MeSH terms: Amino Acids, Blood Glucose, Caseins, Dietary Proteins, Humans, Insulin, Male, Muscle Proteins, Phenylalanine, Sleep, Tyrosine, Muscle, Skeletal, Postprandial Period, Resistance Training, Young Adult

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Resistance Exercise Augments Postprandial Overnight Muscle Protein Synthesis Rates

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