Cited by 76Muscle metabolism and nutritionMuscle Physiology and DisordersSports Performance and Training

Presleep dietary protein-derived amino acids are incorporated in myofibrillar protein during postexercise overnight recovery

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Trommelen, Jorn, Kouw, Imre W. K., Holwerda, Andrew M., Snijders, Tim, Halson, Shona L., Rollo, Ian, Verdijk, Lex B., van Loon, Luc J. C.·2018·American Journal of Physiology-Endocrinology and Metabolism

Abstract

The purpose of this study was to determine the impact of ingesting 30 g casein protein with and without 2 g free leucine before sleep on myofibrillar protein synthesis rates during postexercise overnight recovery. Thirty-six healthy young men performed a single bout of resistance-type exercise in the evening (1945) after a full day of dietary standardization. Thirty minutes before sleep (2330), subjects ingested 30 g intrinsically l-[1-13 C]phenylalanine-labeled protein with (PRO+leu, n = 12) or without (PRO, n = 12) 2 g free leucine, or a noncaloric placebo (PLA, n = 12). Continuous intravenous l-[ ring-2 H 5]phenylalanine, l-[1-13 C]leucine, and l-[ ring-2 H 2]tyrosine infusions were applied. Blood and muscle tissue samples were collected to assess whole body protein net balance, myofibrillar protein synthesis rates, and overnight incorporation of dietary protein-derived amino acids into myofibrillar protein. Protein ingestion before sleep improved overnight whole body protein net balance ( P < 0.001). Myofibrillar protein synthesis rates did not differ significantly between treatments as assessed by l-[ ring-2 H 5]phenylalanine (0.057 ± 0.002, 0.055 ± 0.002, and 0.055 ± 0.004%/h for PLA, PRO, and PRO+leu, respectively; means ± SE; P = 0.850) or l-[1-13 C]leucine (0.080 ± 0.004, 0.073 ± 0.004, and 0.083 ± 0.006%/h, respectively; P = 0.328). Myofibrillar l-[1-13 C]phenylalanine enrichments increased following protein ingestion but did not differ between the PRO and PRO+leu treatments. In conclusion, protein ingestion before sleep improves whole body protein net balance and provides amino acids that are incorporated into myofibrillar protein during sleep. However, the ingestion of 30 g casein protein with or without additional free leucine before sleep does not increase muscle protein synthesis rates during postexercise overnight recovery.

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  1. Jones PR, Pearson J. Anthropometric determination of leg fat and muscle plus bone volumes in young male and female adults. J Physiol 204: 63P-66P, 1969.

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Authors
Trommelen, J., Kouw, I. W. K., Holwerda, A. M., Snijders, T., Halson, S. L., Rollo, I., Verdijk, L. B., & van Loon, L. J. C.
Affiliations
  • Maastricht University Medical Centre (NL)
  • Australian Sports Commission (AU)
  • TiFN (NL)
  • NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands
Venue
American Journal of Physiology-Endocrinology and Metabolism
Published
2018-05-01
Volume
314
Issue
5
Pages
E457-E467
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
OpenAlex
W2618721965
Cited by
76
References
46
Citations by year
2026: 1 · 2025: 7 · 2024: 6 · 2023: 6 · 2022: 6 · 2021: 13 · 2020: 18 · 2019: 11 · 2018: 5 · 2017: 3
FWCI
5.40
Type
journal article
Language
en
Open access
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion, other-oa)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • Research Publications (Maastricht University) (submittedVersion, other-oa)
Funders
TI Food and Nutrition
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
Keywords
recovery sleep, casein, myofibrillar protein synthesis, post-exercise recovery, amino acid incorporation, resistance training, muscle protein synthesis, phenylalanine, tyrosine, leucine, whole-body protein turnover
MeSH terms
Adult, Amino Acids, Basal Metabolism, Circadian Rhythm, Dietary Proteins, Humans, Male, Muscle Proteins, Rest, Sleep, Time Factors, Exercise, Postprandial Period, Recovery of Function, Resistance Training, Young Adult

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