Presleep dietary protein-derived amino acids are incorporated in myofibrillar protein during postexercise overnight recovery
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The purpose of this study was to determine the impact of ingesting 30 g casein protein with and without 2 g free leucine before sleep on myofibrillar protein synthesis rates during postexercise overnight recovery. Thirty-six healthy young men performed a single bout of resistance-type exercise in the evening (1945) after a full day of dietary standardization. Thirty minutes before sleep (2330), subjects ingested 30 g intrinsically l-[1-13 C]phenylalanine-labeled protein with (PRO+leu, n = 12) or without (PRO, n = 12) 2 g free leucine, or a noncaloric placebo (PLA, n = 12). Continuous intravenous l-[ ring-2 H 5]phenylalanine, l-[1-13 C]leucine, and l-[ ring-2 H 2]tyrosine infusions were applied. Blood and muscle tissue samples were collected to assess whole body protein net balance, myofibrillar protein synthesis rates, and overnight incorporation of dietary protein-derived amino acids into myofibrillar protein. Protein ingestion before sleep improved overnight whole body protein net balance ( P < 0.001). Myofibrillar protein synthesis rates did not differ significantly between treatments as assessed by l-[ ring-2 H 5]phenylalanine (0.057 ± 0.002, 0.055 ± 0.002, and 0.055 ± 0.004%/h for PLA, PRO, and PRO+leu, respectively; means ± SE; P = 0.850) or l-[1-13 C]leucine (0.080 ± 0.004, 0.073 ± 0.004, and 0.083 ± 0.006%/h, respectively; P = 0.328). Myofibrillar l-[1-13 C]phenylalanine enrichments increased following protein ingestion but did not differ between the PRO and PRO+leu treatments. In conclusion, protein ingestion before sleep improves whole body protein net balance and provides amino acids that are incorporated into myofibrillar protein during sleep. However, the ingestion of 30 g casein protein with or without additional free leucine before sleep does not increase muscle protein synthesis rates during postexercise overnight recovery.
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A pre-sleep protein dose of ≥40 g has been recommended to sustain MPS rates and minimize overnight muscle protein breakdown.
“a pre-sleep protein dose of ≥ 40 g has been recommended to sustain MPS rates and minimize overnight muscle protein breakdown [75, 76]”
From Nutritional Strategies to Improve Post-exercise Recovery and Subsequent Exercise Performance: A Narrative Review (2025) · cites this paper as [76]
The Discussion (page unavailable) reports that 40 g of casein before sleep increased overnight muscle protein synthesis in previous work, while 30 g did not increase myofibrillar protein synthesis in this study. It calls for research to identify the maximal effective dose; it does not recommend a ≥40 g threshold or show that this dose minimizes overnight muscle protein breakdown. (LLM, conf=0.85)
Experimental — accuracy not yet measuredThere is no need to add extra leucine to pre-sleep casein protein supplementation.
“with no need to add extra leucine [152].”
From The 4R’s Framework of Nutritional Strategies for Post-Exercise Recovery: A Review with Emphasis on New Generation of Carbohydrates (2020) · cites this paper as [152]
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UYKU ÖNCESİ PROTEİN ALIMININ KAS PROTEİN SENTEZİ, KÜTLESİ VE KUVVETİ ÜZERİNE AKUT VE KRONİK ETKİLERİ
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- Authors
- Trommelen, J., Kouw, I. W. K., Holwerda, A. M., Snijders, T., Halson, S. L., Rollo, I., Verdijk, L. B., & van Loon, L. J. C.
- Affiliations
- Maastricht University Medical Centre (NL)
- Australian Sports Commission (AU)
- TiFN (NL)
- NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands
- ORCID
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2018-05-01
- Volume
- 314
- Issue
- 5
- Pages
- E457-E467
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2618721965
- Cited by
- 76
- References
- 46
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 6 · 2023: 6 · 2022: 6 · 2021: 13 · 2020: 18 · 2019: 11 · 2018: 5 · 2017: 3
- FWCI
- 5.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion, other-oa)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Research Publications (Maastricht University) (submittedVersion, other-oa)
- Funders
- TI Food and Nutrition
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
- Keywords
- recovery sleep, casein, myofibrillar protein synthesis, post-exercise recovery, amino acid incorporation, resistance training, muscle protein synthesis, phenylalanine, tyrosine, leucine, whole-body protein turnover
- MeSH terms
- Adult, Amino Acids, Basal Metabolism, Circadian Rhythm, Dietary Proteins, Humans, Male, Muscle Proteins, Rest, Sleep, Time Factors, Exercise, Postprandial Period, Recovery of Function, Resistance Training, Young Adult
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