Cited by 176Gut microbiota and healthInflammatory Bowel DiseaseOral microbiology and periodontitis research

Altered enteric microbiota ecology in interleukin 10-deficient mice during development and progression of intestinal inflammation

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Maharshak, Nitsan, Packey, Christopher D., Ellermann, Melissa, Manick, Sayeed, Siddle, Jennica P., Huh, Eun Young, Plevy, Scott, Sartor, R. Balfour +1 more·2013·Gut Microbes

Abstract

Inflammatory bowel diseases (IBD) result from dysregulated immune responses toward microbial and perhaps other luminal antigens in a genetically susceptible host, and are associated with altered composition and diversity of the intestinal microbiota. The interleukin 10-deficient (IL-10 (-/-) ) mouse has been widely used to model human IBD; however the specific alterations that occur in the intestinal microbiota of this mouse model during the onset of colonic inflammation have not yet been defined. The aim of our study was to define the changes in diversity and composition that occur in the intestinal microbiota of IL-10 (-/-) mice during the onset and progression of colonic inflammation. We used high throughput sequencing of the 16S rRNA gene to characterize the diversity and composition of formerly germ-free, wild-type and IL-10 (-/-) mice associated with the same intestinal microbiota over time. Following two weeks of colonization with a specific pathogen-free (SPF) microbiota we observed a significant increase in the diversity and richness of the intestinal microbiota of wild-type mice. In contrast, a progressive decrease in diversity and richness was observed at three and four weeks in IL-10 (-/-) mice. This decrease in diversity and richness was mirrored by an increase in Proteobacteria and Escherichia coli in IL-10 (-/-) mice. An increase in E. coli was also observed in conventionally raised IL-10 (-/-) mice at the point of colonic inflammation. Our data reports the sequential changes in diversity and composition of the intestinal microbiota in an immune-mediated mouse model that may help provide insights into the primary vs. secondary role of dysbiosis in human IBD patients.

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  • Lmna G609G/G609G 4mo mice exhibit an increment in Mucispirillum, Enterococcus, Acinetobacter, Staphylococcus, Parabacteroides, Bacteroides, Prevotella and Enterobacteriaceae.

    “By contrast, Lmna G609G/G609G 4mo exhibited an increment in Mucispirillum, Enterococcus, Acinetobacter, Staphylococcus, Parabacteroides, Bacteroides, Prevotella and Enterobacteriaceae, which contains the genus Escherichia and has been associated with dysbiosis and intestinal inflammation 20 (Fig. 1d).”

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Authors
Maharshak, N., Packey, C. D., Ellermann, M., Manick, S., Siddle, J. P., Huh, E. Y., Plevy, S., Sartor, R. B., & Carroll, I. M.
Affiliations
University of North Carolina at Chapel Hill (US)
Corresponding author
Carroll, Ian M.
Venue
Gut Microbes
Published
2013-07-12
Volume
4
Issue
4
Pages
316-324
ISSN
  • 1949-0976 (ISSN-L)
  • 1949-0984
OpenAlex
W1997827675
Cited by
174
References
43
Citations by year
2026: 9 · 2025: 18 · 2024: 23 · 2023: 24 · 2022: 12 · 2021: 17 · 2020: 20 · 2019: 15 · 2018: 8 · 2017: 7 · 2016: 7 · 2015: 12 · 2014: 1 · 2013: 1
FWCI
3.14
Type
journal article
Language
en
Open access
Locations
  • Gut Microbes (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (P40OD010995)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01 DK53347, P30 DK 34987-10, P30‐DK34987, R01DK053347, K01DK092330, T32DK07737, T35DK007386, P30DK034987, T32DK007737)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Keywords
interleukin-10 deficiency, gut microbiota, inflammatory bowel disease, colonic inflammation, dysbiosis, microbial diversity, Escherichia coli, Proteobacteria, germ-free mice, microbiota colonization
MeSH terms
Animals, Disease Models, Animal, Inflammatory Bowel Diseases, Interleukin-10, Mice, Knockout, Gastrointestinal Tract, Mice, Biota

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