The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Protospacer recognition motifs: Mixed identities and functional diversity
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PAMs seem to participate in the interference phase and in self/non-self distinction.
“Furthermore, PAMs seem to participate in the interference phase and in self/non-self distinction as well [26].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [26]
The Conclusions and Perspectives section says that interference depends to some extent on PAM sequences, supporting their proposed role in the interference phase. The passages found do not say that PAMs participate in self/non-self distinction; a work with that phrase in its title appears in the References, but that alone does not establish the claim. (LLM, conf=0.84)
Experimental — accuracy not yet measuredCas9 systems from different bacteria recognise other PAM sites.
“the fact that Cas9 systems from different bacteria recognise other PAM sites [26]”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [26]
SpCas9 can recognize a 5′-NGG protospacer adjacent motif (PAM), which is part of the non-self-sequence recognized by the CRISPR bacterial adaptive immune system.
“SpCas9 can recognize a 5′-NGG protospacer adjacent motif (PAM), which is part of the non-self-sequence recognized by the CRISPR bacterial adaptive immune system.”
From Viral Delivery Systems for CRISPR (2019) · cites this paper as [68]
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Citations
Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
RNA-guided genetic silencing systems in bacteria and archaea
Wiedenheft, B., Sternberg, S. H., & Doudna, J. A. (2012) · Nature · doi:10.1038/nature10886 · Cited by 1,851
Flagged first, then by citations
Research works printed but not linked: 66
- 10.1126/science.1179555 · DOI
- 10.1016/j.mib.2011.03.005 · DOI
- 10.1038/nrmicro2577 · DOI · Paper page
- 10.1016/j.tim.2011.08.002 · DOI · Paper page
- 10.1007/s00239-004-0046-3 · DOI · Paper page
- 10.1099/mic.0.27437-0 · DOI · Paper page
- 10.1099/mic.0.28048-0 · DOI
- 10.1128/JB.01415-07 · DOI
- 10.1126/science.1138140 · DOI · Paper page
- 10.1128/JB.01412-07 · DOI
- 10.1093/nar/gks216 · DOI · Paper page
- 10.1371/journal.pone.0035888 · DOI
- 10.1111/j.1365-2958.2012.08171.x · DOI · Paper page
- 10.1073/pnas.112047299 · DOI
- 10.1111/j.1365-2958.2004.04428.x · DOI
- 10.1155/2006/542818 · DOI
- 10.1111/j.1365-2958.2009.06641.x · DOI
- 10.1126/science.1159689 · DOI · Paper page
- 10.1016/j.molcel.2011.10.023 · DOI
- 10.1099/mic.0.023960-0 · DOI · Paper page
- 10.1042/BST0370023 · DOI
- 10.1073/pnas.1104144108 · DOI · Paper page
- 10.1111/j.1365-2958.2010.07452.x · DOI
- 10.1093/nar/gkr606 · DOI
- 10.1074/jbc.M112.377002 · DOI
- 10.1111/j.1365-2958.2012.08172.x · DOI
- 10.1371/journal.pone.0050797 · DOI
- 10.1016/j.molcel.2012.03.020 · DOI
- 10.1111/j.1365-2958.2011.07586.x · DOI
- 10.1073/pnas.1102716108 · DOI
- 10.1038/ncomms1937 · DOI · Paper page
- 10.1016/j.molcel.2012.03.018 · DOI
- 10.1111/j.1462-2920.2010.02266.x · DOI
- 10.1186/gb-2007-8-4-r61 · DOI
- 10.1016/j.str.2009.03.019 · DOI · Paper page
- 10.1111/j.1365-2958.2010.07465.x · DOI · Paper page
- 10.1074/jbc.M112.382598 · DOI
- 10.1074/jbc.M803225200 · DOI
- 10.1186/1745-6150-7-40 · DOI
- 10.1371/journal.pone.0047232 · DOI
- Díez-Villaseñor C, RNA Biol
- 10.1111/j.1365-2958.2012.08170.x · DOI
- 10.1111/j.1365-2958.2010.07073.x · DOI · Paper page
- 10.1111/j.1365-2958.2010.07315.x · DOI
- 10.1111/j.1365-2958.2010.07265.x · DOI
- 10.1016/j.jmb.2012.06.037 · DOI
- 10.1016/j.resmic.2010.09.001 · DOI
- Charbonnier F, 1994, J Bacteriol, 176, 1251, 10.1128/jb.176.5.1251-1259.1994 · DOI
- 10.1099/mic.0.027508-0 · DOI
- 10.1371/journal.pone.0049549 · DOI
- 10.1099/mic.0.036046-0 · DOI
- 10.1128/JB.01487-10 · DOI
- 10.1038/4361101a · DOI
- 10.1038/nature09523 · DOI
- 10.1371/journal.pone.0033802 · DOI
- Tyson GW, 2008, Environ Microbiol, 10, 200, 10.1111/j.1462-2920.2007.01444.x · DOI
- 10.1128/JB.01184-12 · DOI
- 10.1093/nar/gkr1111 · DOI
- 10.1111/mmi.12152 · DOI
- 10.1126/science.1165771 · DOI · Paper page
- 10.1038/nature08703 · DOI
- 10.1016/j.cell.2009.07.040 · DOI · Paper page
- 10.1016/j.molcel.2011.12.013 · DOI
- 10.1074/jbc.M111.238485 · DOI
- 10.1073/pnas.1112832108 · DOI · Paper page
- 10.1146/annurev-genet-110711-155447 · DOI
Cited by
Sorted by citations
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
Development and Applications of CRISPR-Cas9 for Genome Engineering
Hsu, P. D., Lander, E. S., & Zhang, F. (2014) · Cell · doi:10.1016/j.cell.2014.05.010 · Cited by 5,762
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J.-R. J., et al. (2015) · Nature · doi:10.1038/nature14592 · Cited by 1,624
CIRCLE-Seq: A highly sensitive in vitro screen for genome-wide CRISPR-cas9 nuclease off-targets
Tsai, S. Q., Nguyen, N. T., Malagon-Lopez, J., Topkar, V. V., Aryee, M. J., & Joung, J. K. (2017) · Nature Methods · doi:10.1038/nmeth.4278 · Cited by 814
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis
Hou, Z., Zhang, Y., Propson, N. E., Howden, S. E., Chu, L.-F., Sontheimer, E. J., & Thomson, J. A. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1313587110 · Cited by 681
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition
Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Topkar, V. V., Zheng, Z., & Joung, J. K. (2015) · Nature Biotechnology · doi:10.1038/nbt.3404 · Cited by 611
Viral Delivery Systems for CRISPR
Xu, C. L., Ruan, M. Z. C., Mahajan, V. B., & Tsang, S. H. (2019) · Viruses · doi:10.3390/v11010028 · Cited by 277
The discovery of CRISPR in archaea and bacteria
Mojica, F. J. M., & Rodriguez‐Valera, F. (2016) · The FEBS Journal · doi:10.1111/febs.13766 · Cited by 207
Self-citationLoureiro, A., & da Silva, G. J. (2019) · Antibiotics · doi:10.3390/antibiotics8010018 · Cited by 129
PAM-flexible genome editing with an engineered chimeric Cas9
Zhao, L., Koseki, S. R. T., Silverstein, R. A., Amrani, N., Peng, C., Kramme, C., Savic, N., Pacesa, M., Rodríguez, T. C., Stan, T., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-41829-y · Cited by 59
CRISPR-Cas systems in multicellular cyanobacteria
Hou, S., Brenes-Álvarez, M., Reimann, V., Alkhnbashi, O. S., Backofen, R., Muro-Pastor, A. M., & Hess, W. R. (2019) · RNA Biology · doi:10.1080/15476286.2018.1493330 · Cited by 47
Metadata
- Authors
- Shah, S. A., Erdmann, S., Mojica, F. J. M., & Garrett, R. A.
- Venue
- RNA Biology
- Publisher
- Informa UK Limited
- Published
- 2013-02-12
- Volume
- 10
- Issue
- 5
- Pages
- 891-899
- ISSN
- 1547-6286
- 1555-8584
- PMC
- PMC3737346
- Cited by
- 341
- References
- 2
- Language
- en
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