2D and 3D Cell Cultures - a Comparison of Different Types of Cancer Cell Cultures

Matrix elasticity directs stem cell lineage specification

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The third dimension bridges the gap between cell culture and live tissue

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Exploring and Engineering the Cell Surface Interface

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Capturing complex 3D tissue physiology in vitro

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Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines.

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Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels

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Geometric cues for directing the differentiation of mesenchymal stem cells

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Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

Baker, B. M., & Chen, C. S. (2012) · Journal of Cell Science · doi:10.1242/jcs.079509 · Cited by 1,760

Modeling Tissue Morphogenesis and Cancer in 3D

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Spheroid-based drug screen: Considerations and practical approach

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In vitro and in vivo experimental models for cancer immunotherapy study

Zhang, C., Sui, Y., Liu, S., & Yang, M. J. (2024) · Current Research in Biotechnology · doi:10.1016/j.crbiot.2024.100210 · Cited by 44

Clinically relevant radioresistant rhabdomyosarcoma cell lines: functional, molecular and immune-related characterization

Petragnano, F., Pietrantoni, I., Camero, S., Codenotti, S., Milazzo, L., Vulcano, F., Macioce, G., Giordani, I., Tini, P., Cheleschi, S., et al. (2020) · Journal of Biomedical Science · doi:10.1186/s12929-020-00683-6 · Cited by 35

Utility of Three-Dimensional Cultures of Primary Human Hepatocytes (Spheroids) as Pharmacokinetic Models

Mizoi, K., Arakawa, H., Yano, K., Koyama, S., Kojima, H., & Ogihara, T. (2020) · Biomedicines · doi:10.3390/biomedicines8100374 · Cited by 37

The serological responses to acute exercise in humans reduce cancer cell growth in vitro: A systematic review and meta‐analysis

Orange, S. T., Jordan, A., & Saxton, J. (2020) · Physiological Reports · doi:10.14814/phy2.14635 · Cited by 37

Ex Vivo Culture Models to Indicate Therapy Response in Head and Neck Squamous Cell Carcinoma

Demers, I., Donkers, J., Kremer, B., & Speel, E. M. (2020) · Cells · doi:10.3390/cells9112527 · Cited by 35

Three-Dimensional Printing for Cancer Applications: Research Landscape and Technologies

Li, R., Ting, Y.-H., Youssef, S. H., Song, Y., & Garg, S. (2021) · Pharmaceuticals · doi:10.3390/ph14080787 · Cited by 35

In Vitro 3D Modeling of Neurodegenerative Diseases

Louit, A., Galbraith, T., & Berthod, F. (2023) · Bioengineering · doi:10.3390/bioengineering10010093 · Cited by 37

Liver-on-a-chip: Considerations, advances, and beyond

Yang, Z., Liu, X., Cribbin, E. M., Kim, A. M., Li, J. J., & Yong, K. (2022) · Biomicrofluidics · doi:10.1063/5.0106855 · Cited by 36

Canine osteosarcoma in comparative oncology: Molecular mechanisms through to treatment discovery

Simpson, S., Rizvanov, A. A., Jeyapalan, J. N., de Brot, S., & Rutland, C. S. (2022) · Frontiers in Veterinary Science · doi:10.3389/fvets.2022.965391 · Cited by 38

Characterisation of cell lines derived from prostate cancer patients with localised disease

Moya, L., Walpole, C., Rae, F., Srinivasan, S., Seim, I., Lai, J., Nicol, D. L., Williams, E. D., Clements, J. A., & Batra, J. (2023) · Prostate Cancer and Prostatic Diseases · doi:10.1038/s41391-023-00679-x · Cited by 38

Recent Advances in Electrospun Fibers for Biological Applications

Fromager, B., Marhuenda, E., Louis, B., Bakalara, N., Cambedouzou, J., & Cornu, D. (2023) · Macromol—A Journal of Macromolecular Research · doi:10.3390/macromol3030033 · Cited by 34

CRISPR/Cas9: a powerful tool in colorectal cancer research

Hu, Y., Liu, L., Jiang, Q., Fang, W., Chen, Y., Hong, Y., & Zhai, X. (2023) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-023-02901-z · Cited by 33

Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm

Gabriel, V., Zdyrski, C., Sahoo, D. K., Ralston, A., Wickham, H., Bourgois‐Mochel, A., Ahmed, B. H., Merodio, M. M., Paukner, K., Piñeyro, P. E., et al. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25020701 · Cited by 35

3D cell culture using a clinostat reproduces microgravity-induced skin changes

Choi, D. H., Jeon, B., Lim, M. H., Lee, D. H., Ye, S. K., Jeong, S.-Y., & Kim, S. (2021) · npj Microgravity · doi:10.1038/s41526-021-00148-6 · Cited by 34

Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.) as extracellular matrix for tissue engineering applications

Mahendiran, B., Muthusamy, S., Selvakumar, R., Rajeswaran, N., Sampath, S., Jaisankar, S. N., & Krishnakumar, G. S. (2021) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2021.118494 · Cited by 38

Systems for Muscle Cell Differentiation: From Bioengineering to Future Food

Lee, K.-Y., Loh, H. X., & Wan, A. C. A. (2021) · Micromachines · doi:10.3390/mi13010071 · Cited by 35

MCU controls melanoma progression through a redox‐controlled phenotype switch

Stejerean‐Todoran, I., Zimmermann, K., Gibhardt, C. S., Vultur, A., Ickes, C., Shannan, B., del Rio, Z. B., Wölling, A., Cappello, S., Sung, H., et al. (2022) · EMBO Reports · doi:10.15252/embr.202254746 · Cited by 33

Gene expression in organoids: an expanding horizon

Smirnov, A., Melino, G., & Candi, E. (2023) · Biology Direct · doi:10.1186/s13062-023-00360-2 · Cited by 33

3D Cell Models in Radiobiology: Improving the Predictive Value of In Vitro Research

Antonelli, F. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241310620 · Cited by 34

Development of a cancer metastasis-on-chip assay for high throughput drug screening

Özer, L. Y., Fayed, H. S., Ericsson, J., & Zen, A. A. H. (2024) · Frontiers in Oncology · doi:10.3389/fonc.2023.1269376 · Cited by 33

Recent advances in breast cancer cell line research

Sharma, M. P., Shukla, S., & Misra, G. (2024) · International Journal of Cancer · doi:10.1002/ijc.34849 · Cited by 33

ABCB1-dependent collateral sensitivity of multidrug-resistant colorectal cancer cells to the survivin inhibitor MX106-4C

Lei, Z., Albadari, N. G., Teng, Q., Rahman, H., Wang, J., Wu, Z., Ma, D., Ambudkar, S. V., Wurpel, J. N. D., Pan, Y., et al. (2024) · Drug Resistance Updates · doi:10.1016/j.drup.2024.101065 · Cited by 33

Raman Spectroscopy-Based 3D Analysis of Odontogenic Differentiation of Human Dental Pulp Stem Cell Spheroids

Kim, H., Han, Y., Suhito, I. R., Choi, Y. J., Kwon, M., Son, H., Kim, H., & Kim, T. P. (2021) · Analytical Chemistry · doi:10.1021/acs.analchem.0c05165 · Cited by 33

3D bioprinting technology to mimic the tumor microenvironment: tumor-on-a-chip concept

Samadian, H., Jafari, S., Sepand, M. R., Alaei, L., Malvajerd, S. S., Jaymand, M., Ghobadinezhad, F., Jahanshahi, F., Hamblin, M. R., Derakhshankhah, H., et al. (2021) · Materials Today Advances · doi:10.1016/j.mtadv.2021.100160 · Cited by 33

Modelling Human Physiology on-Chip: Historical Perspectives and Future Directions

Pun, S., Haney, L. C., & Barrile, R. (2021) · Micromachines · doi:10.3390/mi12101250 · Cited by 35

Bidirectional Mechanical Response Between Cells and Their Microenvironment

Mierke, C. T. (2021) · Frontiers in Physics · doi:10.3389/fphy.2021.749830 · Cited by 36

From cell spheroids to vascularized cancer organoids: Microfluidic tumor-on-a-chip models for preclinical drug evaluations

Wu, Y., Zhou, Y., Qin, X., & Liu, Y. (2021) · Biomicrofluidics · doi:10.1063/5.0062697 · Cited by 33

Biofabrication of 3D breast cancer models for dissecting the cytotoxic response of human T cells expressing engineered MAIT cell receptors

Dey, M., Kim, M. H., Nagamine, M., Karhan, E., Kozhaya, L., Dogan, M., Unutmaz, D., & Ozbolat, I. T. (2022) · Biofabrication · doi:10.1088/1758-5090/ac925a · Cited by 34

Transcriptomics indicate nuclear division and cell adhesion not recapitulated in MCF7 and MCF10A compared to luminal A breast tumours

Goh, J. J. H., Goh, C. J. H., Lim, Q. W., Zhang, S., Koh, C.-G., & Chiam, K. (2022) · Scientific Reports · doi:10.1038/s41598-022-24511-z · Cited by 33

Breakthroughs and bottlenecks of psoriasis therapy: Emerging trends and advances in lipid based nano-drug delivery platforms for dermal and transdermal drug delivery

Singh, S., & Awasthi, R. (2023) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2023.104548 · Cited by 34

3D melanoma spheroid model for the development of positronium biomarkers

Karimi, H., Moskal, P., Żak, A., & Stępień, E. Ł. (2023) · Scientific Reports · doi:10.1038/s41598-023-34571-4 · Cited by 32

Glycolipid Biosurfactants in Skincare Applications: Challenges and Recommendations for Future Exploitation

Adu, S. A., Twigg, M. S., NAUGHTON, P. J., Marchant, R., & Banat, I. M. (2023) · Molecules · doi:10.3390/molecules28114463 · Cited by 33

Insights on Three Dimensional Organoid Studies for Stem Cell Therapy in Regenerative Medicine

Mulaudzi, P. E., Abrahamse, H., & Crous, A. (2023) · Stem Cell Reviews and Reports · doi:10.1007/s12015-023-10655-6 · Cited by 38

Advances in cancer mechanobiology: Metastasis, mechanics, and materials

Clevenger, A. J., McFarlin, M. K., Gorley, J. P. M., Solberg, S. C., Madyastha, A. K., & Raghavan, S. A. (2024) · APL Bioengineering · doi:10.1063/5.0186042 · Cited by 33

Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

Klak, M., Bryniarski, T., Kowalska, P. D., Gomółka, M., Tymicki, G., Kosowska, K., Cywoniuk, P., Dobrzański, T., Turowski, P., & Wszola, M. (2020) · Micromachines · doi:10.3390/mi11070646 · Cited by 33

Strategies to Potentiate Paracrine Therapeutic Efficacy of Mesenchymal Stem Cells in Inflammatory Diseases

Seo, Y., Kang, M., & Kim, H. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22073397 · Cited by 32

Anti-carcinogenic effects of exercise-conditioned human serum: evidence, relevance and opportunities

Metcalfe, R. S., Kemp, R. N., Heffernan, S. M., Churm, R., Chen, Y., Ruffino, J. S., Conway, G. E., Tornillo, G., & Orange, S. T. (2021) · European Journal of Applied Physiology · doi:10.1007/s00421-021-04680-x · Cited by 33

Intestinal multicellular organoids to study colorectal cancer

Idris, M., Alves, M. M., Hofstra, R. M. W., Mahé, M. M., & Melotte, V. (2021) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2021.188586 · Cited by 33

Advancements, Challenges, and Future Directions in Tackling Glioblastoma Resistance to Small Kinase Inhibitors

Fabro, F., Lamfers, M. L. M., & Leenstra, S. (2022) · Cancers · doi:10.3390/cancers14030600 · Cited by 33

The Study of the Extracellular Matrix in Chronic Inflammation: A Way to Prevent Cancer Initiation?

Marangio, A., Biccari, A., D’Angelo, E., Sensi, F., Spolverato, G., Pucciarelli, S., & Agostini, M. (2022) · Cancers · doi:10.3390/cancers14235903 · Cited by 34

GelMA and Biomimetic Culture Allow the Engineering of Mineralized, Adipose, and Tumor Tissue Human Microenvironments for the Study of Advanced Prostate Cancer In Vitro and In Vivo

Bessot, A., Gunter, J. H., Waugh, D. J. J., Clements, J. A., Hutmacher, D. W., McGovern, J., & Bock, N. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202201701 · Cited by 32

Mechanobiology of cells and cell systems, such as organoids

Bayır, E., Şendemir, A., & Missirlis, Y. F. (2019) · Biophysical Reviews · doi:10.1007/s12551-019-00590-7 · Cited by 35

A 3D Cell Death Assay to Quantitatively Determine Ferroptosis in Spheroids

Demuynck, R., Efimova, I., Lin, A. G., Declercq, H., & Krysko, D. V. (2020) · Cells · doi:10.3390/cells9030703 · Cited by 32

Opportunities and challenges to engineer 3D models of tumor-adaptive immune interactions

Visalakshan, R. M., Lowrey, M. K., Sousa, M. G. C., Helms, H. R., Samiea, A., Schutt, C. E., Moreau, J. M., & Bertassoni, L. E. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1162905 · Cited by 32

Three-dimensional heterotypic colorectal cancer spheroid models for evaluation of drug response

Yau, J. N. N., & Adriani, G. (2023) · Frontiers in Oncology · doi:10.3389/fonc.2023.1148930 · Cited by 32

Rebuilding the microenvironment of primary tumors in humans: a focus on stroma

Mun, S., Lee, H. J., & Kim, P. (2024) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01191-5 · Cited by 31

The Transformative Role of 3D Culture Models in Triple-Negative Breast Cancer Research

Bittman-Soto, X. S., Thomas, E. S., Ganshert, M., Mendez‐Santacruz, L. L., & Harrell, J. C. (2024) · Cancers · doi:10.3390/cancers16101859 · Cited by 31

Development of an automated 3D high content cell screening platform for organoid phenotyping

Bozal, S. B., Sjogren, G., Costa, A. P., Brown, J. S., Roberts, S., Baker, D. S., Gabriel, P., Ristau, B. T., Samuels, M. L., Flynn, W. F., et al. (2024) · SLAS DISCOVERY · doi:10.1016/j.slasd.2024.100182 · Cited by 35

Unravelling the complexities of resistance mechanism in pancreatic cancer: Insights from in vitro and ex-vivo model systems

Lencioni, G., Gregori, A., Toledo, B., Rebelo, R., Immordino, B., Amrutkar, M., Xavier, C. P. R., Kocijancic, A., Pandey, D. P., Perán, M., et al. (2024) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2024.09.002 · Cited by 32

3D bioprinting for the construction of drug testing models-development strategies and regulatory concerns

Mallya, D., Gadre, M. A., Varadharajan, S., & Vasanthan, K. S. (2025) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1457872 · Cited by 33

Biomaterials affect cell-cell interactions in vitro in tissue engineering

He, D. N., & Li, H. (2020) · Journal of Material Science and Technology · doi:10.1016/j.jmst.2020.03.022 · Cited by 32

Advances in Modeling the Immune Microenvironment of Colorectal Cancer

Yoon, P., Piccolo, N. D., Shirure, V. S., Peng, Y., Kirane, A., Canter, R. J., Fields, R. C., George, S. C., & Gholami, S. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2020.614300 · Cited by 33

3D-Printed Collagen Scaffolds Promote Maintenance of Cryopreserved Patients-Derived Melanoma Explants

Jeong, Y., Bang, C. H., Park, M., Shin, S. Y., Yun, S., Kim, C. M., Jeong, G. H., Chung, Y., Yun, W., Lee, J. H., et al. (2021) · Cells · doi:10.3390/cells10030589 · Cited by 31

Organ-on-a-chip platforms as novel advancements for studying heterogeneity, metastasis, and drug efficacy in breast cancer

Kalot, R., Mhanna, R., & Talhouk, R. (2022) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2022.108156 · Cited by 31

Recent advances for using human induced-pluripotent stem cells as pain-in-a-dish models of neuropathic pain

Labau, J. I. R., Anđelić, M., Faber, C. G., Waxman, S. G., Lauria, G., & Dib‐Hajj, S. D. (2022) · Experimental Neurology · doi:10.1016/j.expneurol.2022.114223 · Cited by 32

Engineered cell culture microenvironments for mechanobiology studies of brain neural cells

Ransanz, L. C., van Altena, P. F. J., Heine, V. M., & Accardo, A. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.1096054 · Cited by 31

PLLA scaffolds with controlled architecture as potential microenvironment for in vitro tumor model

Lombardo, M. E., Pavia, F. C., Vitrano, I., Ghersi, G., Brucato, V., Rosei, F., & Carrubba, V. L. (2019) · Tissue and Cell · doi:10.1016/j.tice.2019.04.004 · Cited by 31

Biocompatibility enhancement via post-processing of microporous scaffolds made by optical 3D printer

Jersovaite, J., Šarachovaitė, U., Matulaitienė, I., Niaura, G., Baltriukienė, D., & Malinauskas, M. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1167753 · Cited by 30

Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels

Majumder, J., Torr, E., Aisenbrey, E. A., Lebakken, C. S., Favreau, P. F., Richards, W. D., Yin, Y., Chang, Q., & Murphy, W. L. (2024) · Journal of Tissue Engineering · doi:10.1177/20417314241230633 · Cited by 30

Advantages of Using 3D Spheroid Culture Systems in Toxicological and Pharmacological Assessment for Osteogenesis Research

Yun, C., Kim, S. H., Kim, K. M., Yang, M. H., Byun, M. R., Kim, J., Kwon, D., Pham, H. T. M., Kim, H.-S., Kim, J.-H., et al. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25052512 · Cited by 33

Emergence and Patterning Dynamics of Mouse Definitive Endoderm

Pour, M., Kumar, A. S., Farag, N., Bolondi, A., Kretzmer, H., Walther, M., Wittler, L., Meissner, A., & Nachman, I. (2021) · iScience · doi:10.1016/j.isci.2021.103556 · Cited by 30

Photobiomodulation therapy at red and near-infrared wavelengths for osteogenic differentiation in the scaffold-free microtissues

Çevik, Z. B. Y., Karaman, O., & Topaloğlu, N. (2022) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2022.112615 · Cited by 30

Progress in biomechanical stimuli on the cell-encapsulated hydrogels for cartilage tissue regeneration

Taheri, S., Ghazali, H. S., Ghazali, Z. S., Bhattacharyya, A., & Noh, I. (2023) · Biomaterials Research · doi:10.1186/s40824-023-00358-x · Cited by 30

3D-bioprinted cholangiocarcinoma-on-a-chip model for evaluating drug responses

Liu, Q., Mille, L. S., Villalobos, C., Anaya, I., Vostatek, M., Yi, S., Li, W., Liao, J., Wu, H., Song, Y., et al. (2023) · Bio-Design and Manufacturing · doi:10.1007/s42242-022-00229-9 · Cited by 30

Production of 3D Tumor Models of Head and Neck Squamous Cell Carcinomas for Nanotheranostics Assessment

Santi, M., Mapanao, A. K., Cappello, V., & Voliani, V. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c00617 · Cited by 31

Implications of Oxidative Stress in Glioblastoma Multiforme Following Treatment with Purine Derivatives

Orlicka-Płocka, M., Fedoruk‐Wyszomirska, A., Gurda, D., Pawelczak, P., Krawczyk, P., Giel-Pietraszuk, M., Framski, G., Ostrowski, T., & Wyszko, E. (2021) · Antioxidants · doi:10.3390/antiox10060950 · Cited by 32

Design and Development of a New Type of Hybrid PLGA/Lipid Nanoparticle as an Ursolic Acid Delivery System against Pancreatic Ductal Adenocarcinoma Cells

Markowski, A., Jaromin, A., Migdał, P., Olczak, E., Zygmunt, A., Zaremba-Czogalla, M., Pawlik, K., & Gubernator, J. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23105536 · Cited by 29

Perspectives on the Current State of Bioprinted Skin Substitutes for Wound Healing

Sörgel, C. A., Cai, A., Schmid, R., & Horch, R. E. (2023) · Biomedicines · doi:10.3390/biomedicines11102678 · Cited by 29

Achieving Precision Healthcare through Nanomedicine and Enhanced Model Systems

Svensson, E., von Mentzer, U., & Stubelius, A. (2023) · ACS Materials Au · doi:10.1021/acsmaterialsau.3c00073 · Cited by 30

Assessing Drug Uptake and Response Differences in 2D and 3D Cellular Environments Using Stimulated Raman Scattering Microscopy

Xu, F. X., Sun, R., Owens, R., Hu, K., & Fu, D. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.4c02592 · Cited by 29

3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

Tanadchangsaeng, N., Pasanaphong, K., Tawonsawatruk, T., Rattanapinyopituk, K., Tangketsarawan, B., Rawiwet, V., Kongchanagul, A., Srikaew, N., Yoyruerop, T., Panupinthu, N., et al. (2024) · Scientific Reports · doi:10.1038/s41598-024-73774-1 · Cited by 29

Molecular Profiling of Glioblastoma Patient-Derived Single Cells Using Combined MALDI-MSI and MALDI-IHC

Krestensen, K. K., Hendriks, T., Grgic, A., Derweduwe, M., Smet, F. D., Heeren, R. M. A., & Cuypers, E. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c03821 · Cited by 29

One section, two worlds: single-cell integration of MALDI-MSI and spatial transcriptomics on the same single tissue section

Hendriks, T., Eijkel, G. B., Visvikis, T., Balluff, B., Heeren, R. M. A., & Cuypers, E. (2025) · Scientific Reports · doi:10.1038/s41598-025-26735-1 · Cited by 29

Mammary gland 3D cell culture systems in farm animals

Finot, L., Chanat, E., & Dessauge, F. (2021) · Veterinary Research · doi:10.1186/s13567-021-00947-5 · Cited by 29

Using Biosensors to Study Organoids, Spheroids and Organs-on-a-Chip: A Mechanobiology Perspective

Yousafzai, M. S., & Hammer, J. A. (2023) · Biosensors · doi:10.3390/bios13100905 · Cited by 30

Microfluidics for 3D Cell and Tissue Cultures: Microfabricative and Ethical Aspects Updates

Limongi, T., Guzzi, F., Parrotta, E. I., Candeloro, P., Scalise, S., Lucchino, V., Gentile, F., Tirinato, L., Coluccio, M. L., Torre, B., et al. (2022) · Cells · doi:10.3390/cells11101699 · Cited by 28

The drug efficacy testing in 3D cultures platform identifies effective drugs for ovarian cancer patients

Åkerlund, E., Gudoitytė, G., Moussaud-Lamodière, E. L., Lind, O., Bwanika, H. C., Lehti, K., Salehi, S., Carlson, J. W., Wallin, E., Fernebro, J., et al. (2023) · npj Precision Oncology · doi:10.1038/s41698-023-00463-z · Cited by 28

Extracellular vesicles secreted by 3D tumor organoids are enriched for immune regulatory signaling biomolecules compared to conventional 2D glioblastoma cell systems

Schuster, M., Braun, F., Chiang, D. M., Ludwig, C., Meng, C., Grätz, C., Kirchner, B., Proescholdt, M. A., Hau, P., Steinlein, O. K., et al. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1388769 · Cited by 28

A systematic review on the culture methods and applications of 3D tumoroids for cancer research and personalized medicine

Kalla, J., Pfneissl, J., Mair, T., Tran, L., & Egger, G. (2024) · Cellular Oncology · doi:10.1007/s13402-024-00960-8 · Cited by 31

Challenges in validation of combination treatment strategies for CRC using patient-derived organoids

Benboubker, V., Ramzy, G. M., Jacobs, S., & Nowak-Sliwinska, P. M. (2024) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-024-03173-x · Cited by 29

Engineered cell-laden alginate microparticles for 3D culture

Namgung, B., Ravi, K., Vikraman, P. P., Sengupta, S., & Jang, H. L. (2021) · Biochemical Society Transactions · doi:10.1042/bst20200673 · Cited by 28

Strategies for developing complex multi-component in vitro tumor models: Highlights in glioblastoma

DePalma, T. J., Sivakumar, H., & Skardal, A. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.114067 · Cited by 30

Exploring the function of stromal cells in cholangiocarcinoma by three-dimensional bioprinting immune microenvironment model

Li, C., Jin, B., Sun, H., Wang, Y., Zhao, H., Sang, X., Yang, H., & Mao, Y. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.941289 · Cited by 28

Tissue-Engineered Models of the Human Brain: State-of-the-Art Analysis and Challenges

Tarricone, G., Carmagnola, I., & Chiono, V. (2022) · Journal of Functional Biomaterials · doi:10.3390/jfb13030146 · Cited by 30

The Three-Dimensional In Vitro Cell Culture Models in the Study of Oral Cancer Immune Microenvironment

Abdolahinia, E. D., & Han, X. (2023) · Cancers · doi:10.3390/cancers15174266 · Cited by 29

Tumor microenvironment – Unknown niche with powerful therapeutic potential

Kolenda, T., Przybyła, W., Kapałczyńska, M., Teresiak, A., Zajączkowska, M. M., Bliźniak, R., & Lamperska, K. M. (2018) · Reports of Practical Oncology & Radiotherapy · doi:10.1016/j.rpor.2018.01.004 · Cited by 29

Engineering and In Vitro Selection of a Novel AAV3B Variant with High Hepatocyte Tropism and Reduced Seroreactivity

Biswas, M., Marsic, D., Li, N., Zou, C., González‐Aseguinolaza, G., Zolotukhin, I., Kumar, S. R. P., Rana, J., Butterfield, J. S. S., Kondratov, O., et al. (2020) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2020.09.019 · Cited by 28

Cytotoxicity of Seaweed Compounds, Alone or Combined to Reference Drugs, against Breast Cell Lines Cultured in 2D and 3D

Malhão, F., Ramos, A. A., Macedo, A. C., & Rocha, E. (2021) · Toxics · doi:10.3390/toxics9020024 · Cited by 28

Understanding current experimental models of glioblastoma-brain microenvironment interactions

Yadav, N., & Purow, B. W. (2024) · Journal of Neuro-Oncology · doi:10.1007/s11060-023-04536-8 · Cited by 32

Advances in, and prospects of, 3D preclinical models for skin drug discovery

Imran, M., Moyle, P. M., Kamato, D., & Mohammed, Y. (2024) · Drug Discovery Today · doi:10.1016/j.drudis.2024.104208 · Cited by 30

Engineering confining microenvironment for studying cancer metastasis

Jiang, K., Liang, L., & Lim, C. T. (2021) · iScience · doi:10.1016/j.isci.2021.102098 · Cited by 27

PD-1/PD-L1 checkpoint inhibitors in combination with olaparib display antitumor activity in ovarian cancer patient-derived three-dimensional spheroid cultures

Appleton, K. M., Elrod, A. K., Lassahn, K. A., Shuford, S., Holmes, L. M., & DesRochers, T. M. (2021) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-021-02849-z · Cited by 27

Organoid biobanks as a new tool for pre-clinical validation of candidate drug efficacy and safety.

Botti, G., Bonito, M. D., & Cantile, M. (2021) · PubMed Central · Cited by 27

Proteomics in systems toxicology

Madeira, C., & Costa, P. M. (2021) · Advances in protein chemistry and structural biology · doi:10.1016/bs.apcsb.2021.03.001 · Cited by 28

The challenge of ovarian tissue culture: 2D versus 3D culture

Pais, A. S., Reis, S. A., Laranjo, M., Caramelo, F. J., Silva, A. I., Botelho, M. F., & Almeida‐Santos, T. (2021) · Journal of Ovarian Research · doi:10.1186/s13048-021-00892-z · Cited by 30

Targeted Delivery of Cabazitaxel Using Cyclic Cell-Penetrating Peptide and Biomarkers of Extracellular Matrix for Prostate and Breast Cancer Therapy

Park, S. E., El‐Sayed, N. S., Shamloo, K., Lohan, S., Kumar, S., Sajid, M. I., & Tiwari, R. K. (2021) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.1c00319 · Cited by 27

Glycomaterials to Investigate the Functional Role of Aberrant Glycosylation in Glioblastoma

Tondepu, C., & Karumbaiah, L. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202101956 · Cited by 27

Epithelial and Mesenchymal Features of Pancreatic Ductal Adenocarcinoma Cell Lines in Two- and Three-Dimensional Cultures

Shichi, Y., Gomi, F., Sasaki, N., Nonaka, K., Arai, T., & Ishiwata, T. (2022) · Journal of Personalized Medicine · doi:10.3390/jpm12050746 · Cited by 27

Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

Sahi, A. K., Gundu, S., Kumari, P., Klepka, T., & Sionkowska, A. (2023) · Biomimetics · doi:10.3390/biomimetics8010055 · Cited by 27

Harnessing three-dimensional (3D) cell culture models for pulmonary infections: State of the art and future directions

Shah, D. D., Raghani, N. R., Chorawala, M. R., Singh, S. K., & Prajapati, B. G. (2023) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/s00210-023-02541-2 · Cited by 30

Three-Dimensional Cell Culture at the Frontiers of in Vitro Cancer Research: Present Perspectives

Soo, L. L., Zainal, A. A., Hidetsugu, T., Takabatake, K., Nakano, K., Chai, W. L., Nagatsuka, H., & Siar, C. H. (2016) · Annals of Dentistry · doi:10.22452/adum.vol23no1.1

Why 3D in vitro cancer models are the future of cancer research?

Petrić, T., & Sabol, M. (2023) · Periodicum Biologorum · doi:10.18054/pb.v124i3-4.24697 · Cited by 3

Life is Three Dimensional—As In Vitro Cancer Cultures Should Be

Levinger, I., Ventura, Y., & Vago, R. (2014) · Advances in cancer research · doi:10.1016/b978-0-12-800249-0.00009-3 · Cited by 45

Current Perspectives on 2D and 3D Cell Culture Models in Cancer Research: Molecular Determinants of Tumor Biology and Therapeutic Response

YILDIRIM, S., Gómez-Salgado, J. C., Khoury-Moreno, L. E., & Özden, Ö. (2026) · Current Issues in Molecular Biology · doi:10.3390/cimb48090914

2D and 3D cell culture: Getting close to mimicking the tumor microenvironment in vitro

Jain, S., Raza, K., Agrawal, A. K., & Vaidya, A. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-817846-1.00027-8