2D and 3D Cell Cultures - a Comparison of Different Types of Cancer Cell Cultures

Matrix elasticity directs stem cell lineage specification

Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915

The third dimension bridges the gap between cell culture and live tissue

Pampaloni, F., Reynaud, E. G., & Stelzer, E. H. K. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2236 · Cited by 2,817

Exploring and Engineering the Cell Surface Interface

Stevens, M. M., & George, J. H. (2005) · Science · doi:10.1126/science.1106587 · Cited by 2,607

Capturing complex 3D tissue physiology in vitro

Griffith, L. G., & Swartz, M. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1858 · Cited by 2,347

Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines.

Scudiero, D. A., Shoemaker, R. H., Paull, K. D., Monks, A. P., Tierney, S., Nofziger, T. H., Currens, M. J., Seniff, D. A., & Boyd, M. R. (1988) · PubMed · Cited by 2,342

Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels

Benya, P. D., & Shaffer, J. D. (1982) · Cell · doi:10.1016/0092-8674(82)90027-7 · Cited by 2,284

Geometric cues for directing the differentiation of mesenchymal stem cells

Kilian, K. A., Bugarija, B., Lahn, B. T., & Mrksich, M. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0903269107 · Cited by 1,841

Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

Baker, B. M., & Chen, C. S. (2012) · Journal of Cell Science · doi:10.1242/jcs.079509 · Cited by 1,760

Modeling Tissue Morphogenesis and Cancer in 3D

Yamada, K. M., & Cukierman, E. (2007) · Cell · doi:10.1016/j.cell.2007.08.006 · Cited by 1,751

Spheroid-based drug screen: Considerations and practical approach

Friedrich, J., Seidel, C., Ebner, R., & Kunz-Schughart, L. A. (2009) · Nature Protocols · doi:10.1038/nprot.2008.226 · Cited by 1,742

Advances in screening hyperthermic nanomedicines in 3D tumor models

Soeiro, J. F., Sousa, F. L., Monteiro, M. V., Gaspar, V. M., Silva, N. J. O., & Mano, J. F. (2024) · Nanoscale Horizons · doi:10.1039/d3nh00305a · Cited by 27

A Systematic Review on Organ-on-a-Chip in PDMS or Hydrogel in Dentistry: An Update of the Literature

Pierfelice, T. V., D’Amico, E., Petrini, M., Romano, M., D’Arcangelo, C., Sbordone, L., Barone, A. A., Plebani, R., & Iezzi, G. (2024) · Gels · doi:10.3390/gels10020102 · Cited by 26

REGγ potentiates TGF-β/Smad signal dependent epithelial-mesenchymal transition in thyroid cancer cells

Bhatti, M. Z., Pan, L., Wang, T., Shi, P., & Li, L. (2019) · Cellular Signalling · doi:10.1016/j.cellsig.2019.109412 · Cited by 27

Porous Polymeric Microspheres With Controllable Pore Diameters for Tissue Engineered Lung Tumor Model Development

Dhamecha, D. L., Le, D., Movsas, R., Gonsalves, A., & Menon, J. U. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00799 · Cited by 26

Hepatitis E Virus Infection—Immune Responses to an Underestimated Global Threat

Kupke, P., & Werner, J. M. (2021) · Cells · doi:10.3390/cells10092281 · Cited by 28

Bioprinting and its Use in Tumor-On-A-Chip Technology for Cancer Drug Screening: A Review

Fang, L., Liu, Y., Qiu, J., & Wan, W. (2022) · International Journal of Bioprinting · doi:10.18063/ijb.v8i4.603 · Cited by 27

Fractionation and Characterization of Triterpenoids from Vaccinium vitis-idaea L. Cuticular Waxes and Their Potential as Anticancer Agents

Vilkickytė, G., Petrikaitė, V., Marksa, M., Іванаускас, Л., Jakštas, V., & Raudonė, L. (2023) · Antioxidants · doi:10.3390/antiox12020465 · Cited by 26

Recent advances in 3D bioprinted tumor models for personalized medicine

Gnatowski, P., Piłat, E., Kucińska‐Lipka, J., Saeb, M. R., Hamblin, M. R., & Mozafari, M. (2023) · Translational Oncology · doi:10.1016/j.tranon.2023.101750 · Cited by 26

Gaining New Biological and Therapeutic Applications into the Liver with 3D In Vitro Liver Models

Lee, S. W., Jung, D. J., & Jeong, G. S. (2020) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-020-00245-9 · Cited by 26

Drug Delivery of Anticancer Drugs from Injectable 3D Porous Silk Scaffold for Prevention of Gastric Cancer Growth and Recurrence

Gangrade, A., & Mandal, B. B. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c01043 · Cited by 26

Spheroids and organoids derived from colorectal cancer as tools for in vitro drug screening

Zawawi, S. S. A., Salleh, E. A., & Musa, M. (2024) · Exploration of Targeted Anti-tumor Therapy · doi:10.37349/etat.2024.00226 · Cited by 25

Effect of 3D and 2D cell culture systems on trophoblast extracellular vesicle physico-chemical characteristics and potency

Khan, N. L. A., Muhandiram, S., Dissanayake, K., Godakumara, K., Midekessa, G., Andronowska, A., Heath, P. R., Kodithuwakku, S. P., Hart, A. R., & Fazeli, A. (2024) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2024.1382552 · Cited by 26

Simplified low-cost methodology to establish, histologically process and analyze three-dimensional cancer cell spheroid arrays

Moraes, G. D. S., Wink, M. R., Klamt, F., Silva, A. O., & Fernandes, M. D. C. (2020) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2020.151095 · Cited by 25

Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells

Furuhashi, T., Ishii, R., Onishi, H., & Ota, S. (2020) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2020.00116 · Cited by 25

Polyglycerol Ester-Based Low Energy Nanoemulsions with Red Raspberry Seed Oil and Fruit Extracts: Formulation Development toward Effective In Vitro/In Vivo Bioperformance

Gledović, A., Ležaić, A. J., Nikolić, I., Kostov, M. T., Antić‐Stanković, J., Krstonosic, V. S., Randjelovic, D. V., Božić, D. D., Ilić, D., Tamburić, S., et al. (2021) · Nanomaterials · doi:10.3390/nano11010217 · Cited by 25

Fibrous Hydrogels under Multi‐Axial Deformation: Persistence Length as the Main Determinant of Compression Softening

de Almeida, P., Janmey, P. A., & Kouwer, P. H. J. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202010527 · Cited by 25

Double emulsion-pretreated microwell culture for the in vitro production of multicellular spheroids and their in situ analysis

Qu, F., Zhao, S., Cheng, G., Rahman, H., Xiao, Q., Chan, R. W. Y., & Ho, Y. (2021) · Microsystems & Nanoengineering · doi:10.1038/s41378-021-00267-w · Cited by 27

Development of patient‑derived tumor organoids and a drug testing model for renal cell carcinoma

Kazama, A., Anraku, T., Kuroki, H., Shirono, Y., Murata, M., Bilim, V. N., Ugolkov, A. V., Saito, K., & Tomita, Y. (2021) · Oncology Reports · doi:10.3892/or.2021.8177 · Cited by 26

Looking for In Vitro Models for Retinal Diseases

Alfonsetti, M., Castelli, V., d’Angelo, M., Benedetti, E., Allegretti, M., Barboni, B., & Cimini, A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms221910334 · Cited by 27

Engineering Biological Tissues from the Bottom-Up: Recent Advances and Future Prospects

Wang, X., Wang, Z., Zhai, W., Wang, F., Ge, Z., Yu, H., & Yang, W. (2021) · Micromachines · doi:10.3390/mi13010075 · Cited by 26

Development and characterisation of 3D collagen-gelatin based scaffolds for breast cancer research

Redmond, J., McCarthy, H. O., Buchanan, P., Levingstone, T. J., & Dunne, N. (2022) · Biomaterials Advances · doi:10.1016/j.bioadv.2022.213157 · Cited by 26

Three-Dimensional Human Bone Marrow Organoids for the Study and Application of Normal and Abnormal Hematoimmunopoiesis

de Janon, A., Mantalaris, A., & Panoskaltsis, N. (2023) · The Journal of Immunology · doi:10.4049/jimmunol.2200836 · Cited by 26

Cell Culture Model Evolution and Its Impact on Improving Therapy Efficiency in Lung Cancer

Roman, V., Mihaila, M. A., Radu, N., Marineata, S., Diaconu, C. C., & Bostan, M. (2023) · Cancers · doi:10.3390/cancers15204996 · Cited by 26

Doxorubicin-sensitive and -resistant colorectal cancer spheroid models: assessing tumor microenvironment features for therapeutic modulation

Valente, R., Cordeiro, S. Z., Luz, A., Melo, M. C., Roma‐Rodrigues, C., Baptista, P. V., & Fernandes, A. R. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2023.1310397 · Cited by 25

Tumor-on-a-chip platforms for assessing nanoparticle-based cancer therapy

Wang, Y., Cuzzucoli, F., Escobar, A., Lu, S., Liang, L., & Wang, S. (2018) · Nanotechnology · doi:10.1088/1361-6528/aac7a4 · Cited by 26

A microwell platform for high-throughput longitudinal phenotyping and selective retrieval of organoids

Sockell, A., Wong, W. H., Longwell, S. A., Vu, T., Karlsson, K., Mokhtari, D. A., Schaepe, J. M., Lo, Y., Cornelius, V., Kuo, C. J., et al. (2023) · Cell Systems · doi:10.1016/j.cels.2023.08.002 · Cited by 25

Revolutionizing gut health: advances in encapsulation strategies for probiotics and bioactive molecules

Martelli, A., Mohamed, Y. O. A., Ferrer, G. G., Gentile, P., & Girón‐Hernández, J. (2025) · Biotechnology Advances · doi:10.1016/j.biotechadv.2025.108630 · Cited by 25

Photonic crystal biosensor featuring an eye-shaped cavity for precise identification of cancerous cells

Rizk, S., Abd-Elsamee, S., Marzouk, E. S. A., & Areed, N. F. F. (2025) · Scientific Reports · doi:10.1038/s41598-025-07938-y · Cited by 24

Systems Biology and Experimental Model Systems of Cancer

Yalçın, G. D., Danisik, N., Baygin, R. C., & Acar, A. (2020) · Journal of Personalized Medicine · doi:10.3390/jpm10040180 · Cited by 24

An Overview of In Vitro, In Vivo, and Computational Techniques for Cancer‐Associated Angiogenesis Studies

Rahman, H. S., Tan, B. L., Othman, H. H., Chartrand, M. S., Pathak, Y. V., Mohan, S., Abdullah, R., & Alitheen, N. B. (2020) · BioMed Research International · doi:10.1155/2020/8857428 · Cited by 24

Two-Dimensional and Three-Dimensional Cartilage Model Platforms for Drug Evaluation and High-Throughput Screening Assays

Foster, N. C., Hall, N. M., & Haj, A. J. E. (2021) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2020.0354 · Cited by 24

Urine-Derived Epithelial Cells as Models for Genetic Kidney Diseases

Bondue, T., Arcolino, F. O., Veys, K. R. P., Adebayo, O. C., Levtchenko, E. N., van den Heuvel, L. P., & Elmonem, M. A. (2021) · Cells · doi:10.3390/cells10061413 · Cited by 24

Models for Translational Proton Radiobiology—From Bench to Bedside and Back

Suckert, T., Nexhipi, S., Dietrich, A., Koch, R. A., Kunz‐Schughart, L. A., Bahn, E., & Beyreuther, E. (2021) · Cancers · doi:10.3390/cancers13164216 · Cited by 26

Spontaneous Formation of 3D Breast Cancer Tissues on Electrospun Chitosan/Poly(ethylene oxide) Nanofibrous Scaffolds

Rabie, A. M. I., Ali, A. S. M., Al‐Zeer, M. A., Barhoum, A., El-hallouty, S. M., Shousha, W. G., Berg, J., Kurreck, J., & Khalil, A. S. G. (2022) · ACS Omega · doi:10.1021/acsomega.1c05646 · Cited by 24

Evaluation of immunotherapies improving macrophage anti-tumor response using a microfluidic model

Chernyavska, M., Hermans, C. K. J. C., Chan, C., Baumann, N., Rösner, T., Leusen, J. H. W., Valerius, T., & Verdurmen, W. P. R. (2022) · Organs-on-a-Chip · doi:10.1016/j.ooc.2022.100019 · Cited by 25

Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

Gabriel, V., Zdyrski, C., Sahoo, D. K., Dao, K., Bourgois‐Mochel, A., Atherly, T. A., Martinez, M. N., Volpe, D. A., Kopper, J. J., Allenspach, K., et al. (2022) · Journal of Visualized Experiments · doi:10.3791/63612 · Cited by 24

3D Bioprinting: An Enabling Technology to Understand Melanoma

Fernandes, S., Vyas, C., Lim, P., Pereira, R. F., Virós, A., & Bártolo, P. (2022) · Cancers · doi:10.3390/cancers14143535 · Cited by 25

Brazilian red propolis extract free and encapsulated into polymeric nanoparticles against ovarian cancer: formulation, characterisation and biological assays in 2D and 3D models

Justino, I. A., Marincek, A., Ferreira, I. R. S., do Amaral, R. L. F., Fontanezi, B. B., Mejía, J. A. A., Bastos, J. K., & Marcato, P. D. (2023) · Journal of Pharmacy and Pharmacology · doi:10.1093/jpp/rgad030 · Cited by 24

Nanostructured lipid carriers loaded into in situ gels for breast cancer local treatment

Passos, J. S., Apolinário, A. C., Ishida, K., Martins, T. S., & Lopes, L. B. (2023) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2023.106638 · Cited by 24

Mesenchymal stem cells lose the senescent phenotype under 3D cultivation

Krasnova, O. A., Kovaleva, A., Saveleva, A., Kulakova, K. A., Bystrova, O. A., Martynova, M. G., Domnina, A. P., Sopova, J. V., & Neganova, I. E. (2023) · Stem Cell Research & Therapy · doi:10.1186/s13287-023-03599-8 · Cited by 24

Harnessing the Potential of Stem Cells for Disease Modeling: Progress and Promises

Argentati, C., Tortorella, I., Bazzucchi, M., Morena, F., & Martino, S. (2020) · Journal of Personalized Medicine · doi:10.3390/jpm10010008 · Cited by 24

Characterization of an Alginate Encapsulated LS180 Spheroid Model for Anti-colorectal Cancer Compound Screening

Smit, T., Calitz, C., Willers, C., Svitina, H. M., Hamman, J. H., Fey, S. J., Gouws, C., & Wrzesinski, K. (2020) · ACS Medicinal Chemistry Letters · doi:10.1021/acsmedchemlett.0c00076 · Cited by 25

Engineering Cell‐Derived Matrices: From 3D Models to Advanced Personalized Therapies

Rubí-Sans, G., Castaño, Ó., Cano, I., Mateos‐Timoneda, M. A., Pérez‐Amodio, S., & Engel, E. (2020) · Advanced Functional Materials · doi:10.1002/adfm.202000496 · Cited by 26

A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro

Keller, F., Bruch, R., Schneider, R., Meier-Hubberten, J. C., Hafner, M., & Rudolf, R. (2020) · Cells · doi:10.3390/cells9081900 · Cited by 24

Current Landscape in Organic Nanosized Materials Advances for Improved Management of Colorectal Cancer Patients

Ginghină, O., Hudiță, A., Zaharia, C., Tsatsakis, A. M., Mezhuev, Y. O., Costache, M., & Gălățeanu, B. (2021) · Materials · doi:10.3390/ma14092440 · Cited by 24

Advances in microfluidic 3D cell culture for preclinical drug development

(2022) · Progress in Molecular Biology and Translational Science · doi:10.1016/bs.pmbts.2021.07.022 · Cited by 23

Sensory neurons increase keratinocyte proliferation through CGRP release in a tissue engineered in vitro model of innervation in psoriasis

Pépin, R., Ringuet, J., Beaudet, M., Bellenfant, S., Galbraith, T., Veillette, H., Pouliot, R., & Berthod, F. (2024) · Acta Biomaterialia · doi:10.1016/j.actbio.2024.05.021 · Cited by 23

Gradient-induced instability in tumour spheroids unveils the impact of microenvironmental nutrient changes

Ascione, F., Ferraro, R., Dogra, P., Vittorio Cristini, C., Guido, S., & Caserta, S. (2024) · Scientific Reports · doi:10.1038/s41598-024-69570-6 · Cited by 23

Development of droplet-based microfluidics in generation, manipulation and biomedical application

Cai, Y., Sun, R., Ren, Y., & Gou, Y. (2025) · Microchemical Journal · doi:10.1016/j.microc.2025.113101 · Cited by 23

An overview of bio-actuation in collagen hydrogels: a mechanobiological phenomenon

Hameed, P., & Manivasagam, G. (2021) · Biophysical Reviews · doi:10.1007/s12551-021-00804-x · Cited by 26

Development of an in vitro neuroblastoma 3D model and its application for sterigmatocystin-induced cytotoxicity testing

Zingales, V., Torriero, N., Zanella, L., Fernández-Franzón, M., Ruiz, M.-J., Esposito, M. R., & Cimetta, E. (2021) · Food and Chemical Toxicology · doi:10.1016/j.fct.2021.112605 · Cited by 23

A critical analysis of research methods and biological experimental models to study pulp regeneration

Rosa, V., Sriram, G., McDonald, N. J., & Cavalcanti, B. N. (2022) · International Endodontic Journal · doi:10.1111/iej.13712 · Cited by 23

3D Models of Cellular Spheroids As a Universal Tool for Studying the Cytotoxic Properties of Anticancer Compounds In Vitro

Sogomonyan, A. S., Shipunova, V. O., Soloviev, V. D., Larionov, V., Kotelnikova, P. A., & Deyev, S. M. (2022) · Acta Naturae · doi:10.32607/actanaturae.11603 · Cited by 23

Patient-Derived Multiple Myeloma 3D Models for Personalized Medicine—Are We There Yet?

Lourenço, D., Lopes, R., Pestana, C., Queirós, A. C., Joao, C. M., & Carneiro, E. A. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232112888 · Cited by 24

Brain organoid-on-a-chip: A next-generation human brain avatar for recapitulating human brain physiology and pathology

Song, J., Bang, S., Choi, N., & Kim, H. N. (2022) · Biomicrofluidics · doi:10.1063/5.0121476 · Cited by 25

SPRED2: A Novel Regulator of Epithelial-Mesenchymal Transition and Stemness in Hepatocellular Carcinoma Cells

Gao, T., Xu, Y., Fujisawa, M., Ohara, T., Wang, T., Tomonobu, N., Sakaguchi, M., Yoshimura, T., & Matsukawa, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24054996 · Cited by 24

3D-Printed Tumor-on-Chip for the Culture of Colorectal Cancer Microspheres: Mass Transport Characterization and Anti-Cancer Drug Assays

Sánchez-Salazar, M. G., Oliver, R. C.-L., Ramos-Meizoso, S., Jerezano-Flores, V. S., Gallegos-Martínez, S., Bolívar‐Monsalve, E. J., Ceballos‐González, C. F., Santiago, G. T., & Álvarez, M. M. (2023) · Bioengineering · doi:10.3390/bioengineering10050554 · Cited by 23

The feasible application of microfluidic tissue/organ-on-a-chip as an impersonator of oral tissues and organs: a direction for future research

Farshidfar, N., Assar, S., Amiri, M. A., Sahmeddini, S., Hamedani, S., Zarei, M., & Tayebi, L. (2023) · Bio-Design and Manufacturing · doi:10.1007/s42242-023-00235-5 · Cited by 24

Step-by-step fabrication of heart-on-chip systems as models for cardiac disease modeling and drug screening

Deir, S., Mofrad, Y. M., Mashayekhan, S., Shamloo, A., & Mansoori-Kermani, A. (2023) · Talanta · doi:10.1016/j.talanta.2023.124901 · Cited by 23

In Vitro Models of Head and Neck Cancer: From Primitive to Most Advanced

Arutyunyan, I. V., Jumaniyazova, E. D., Макаров, А. В., & Fatkhudinov, T. (2023) · Journal of Personalized Medicine · doi:10.3390/jpm13111575 · Cited by 23

Applications of Engineered Skin Tissue for Cosmetic Component and Toxicology Detection

Wang, M., Zhang, L., Hao, H., Yan, M., & Zhu, Z. (2024) · Cell Transplantation · doi:10.1177/09636897241235464 · Cited by 23

A microfluidic co-culture model for investigating colonocytes–microbiota interactions in colorectal cancer

Penarete-Acosta, D., Stading, R. E., Emerson, L., Horn, M., Chakraborty, S., Han, A., & Jayaraman, A. (2024) · Lab on a Chip · doi:10.1039/d4lc00013g · Cited by 23

Cytotoxic and molecular differences of anticancer agents on 2D and 3D cell culture

Alwahsh, M., Al-Doridee, A., Jasim, S., Awwad, O., Hergenröder, R., & Hamadneh, L. (2024) · Molecular Biology Reports · doi:10.1007/s11033-024-09669-1 · Cited by 25

Towards optimized breast cancer 3D spheroid mono- and co-culture models for pharmacological research and screening

Keller, F., Rudolf, R., & Hafner, M. (2019) · Journal of Cellular Biotechnology · doi:10.3233/jcb-199001 · Cited by 23

Evaluation of Melanoma (SK-MEL-2) Cell Growth between Three-Dimensional (3D) and Two-Dimensional (2D) Cell Cultures with Fourier Transform Infrared (FTIR) Microspectroscopy

Srisongkram, T., Weerapreeyakul, N., & Thumanu, K. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21114141 · Cited by 23

Engineering Biomaterials to Guide Heart Cells for Matured Cardiac Tissue

Jang, Y., Park, Y., & Kim, J. (2020) · Coatings · doi:10.3390/coatings10100925 · Cited by 25

Biomaterial strategies to replicate gynecological tissue

Cadena, I., Chen, A. L., Arvidson, A., & Fogg, K. C. (2020) · Biomaterials Science · doi:10.1039/d0bm01240h · Cited by 23

Prospective prediction of clinical drug response in high-grade gliomas using an ex vivo 3D cell culture assay

Shuford, S., Lipinski, L., Abad, A. P., Smith, A. M., Rayner, M., O’Donnell, L., Stuart, J., Mechtler, L. L., Fabiano, A. J., Edenfield, J., et al. (2021) · Neuro-Oncology Advances · doi:10.1093/noajnl/vdab065 · Cited by 23

Characterization and quantification of necrotic tissues and morphology in multicellular ovarian cancer tumor spheroids using optical coherence tomography

Yan, F., Gunay, G., Valerio, T. I., Wang, C., Wilson, J. A., Haddad, M., Watson, M., Connell, M. O., Davidson, N., Fung, K.-M. A., et al. (2021) · Biomedical Optics Express · doi:10.1364/boe.425512 · Cited by 23

Advancements in chondrocyte 3-dimensional embedded culture: Implications for tissue engineering and regenerative medicine

Chu, Y.-Y., Hikita, A., Asawa, Y., & Hoshi, K. (2024) · Biomedical Journal · doi:10.1016/j.bj.2024.100786 · Cited by 22

Green synthesized Zingiber officinale -ZnO nanoparticles: anticancer efficacy against 3D breast cancer model

Alhaddad, R., Abualsoud, B. M., Al‐Deeb, I., & Nsairat, H. (2024) · Future Science OA · doi:10.1080/20565623.2024.2419806 · Cited by 22

Advanced 3D scaffolds for corneal stroma regeneration: a preclinical progress

Salehi, A. O. M., Soleimani, M., Rios, K. S. C., Ortega-Lara, W. D. L., & Mamidi, N. (2025) · Journal of Materials Chemistry B · doi:10.1039/d5tb00090d · Cited by 22

Bringing ophthalmology into the scientific world: Novel nanoparticle-based strategies for ocular drug delivery

Abbasi, M., Aghamollaei, H., Vaez, A., Amani, A. M., Kamyab, H., Chelliapan, S., Jamalpour, S., & Zambrano-Dávila, R. (2025) · The Ocular Surface · doi:10.1016/j.jtos.2025.03.004 · Cited by 23

Biomarkers and cell-based models to predict the outcome of neoadjuvant therapy for rectal cancer patients

Alkan, A., Hofving, T., Angenete, E., & Yrlid, U. (2021) · Biomarker Research · doi:10.1186/s40364-021-00313-9 · Cited by 22

Head and neck cancer patient-derived tumouroid cultures: opportunities and challenges

Basnayake, B. W. M. T. J., Leo, P., Rao, S., Vasani, S. S., Kenny, L., Haass, N. K., & Punyadeera, C. (2023) · British Journal of Cancer · doi:10.1038/s41416-023-02167-4 · Cited by 23

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Malandain, N., Sanz-Fraile, H., Farré, R., Otero, J., Roig, A., & Laromaine, A. (2023) · ACS Applied Bio Materials · doi:10.1021/acsabm.3c00126 · Cited by 22

Growing Role of 3D In Vitro Cell Cultures in the Study of Cellular and Molecular Mechanisms: Short Focus on Breast Cancer, Endometriosis, Liver and Infectious Diseases

Bloise, N., Giannaccari, M., Guagliano, G., Peluso, E., Restivo, E., Strada, S., Volpini, C., Petrini, P., & Visai, L. (2024) · Cells · doi:10.3390/cells13121054 · Cited by 24

Decoding senescence of aging single cells at the nexus of biomaterials, microfluidics, and spatial omics

Venkataraman, A., Kordic, I., Li, J., Zhang, N. Y., Bharadwaj, N. S., Fang, Z., Das, S., & Coskun, A. F. (2024) · npj Aging · doi:10.1038/s41514-024-00178-w · Cited by 21

Polymersome‐assisted delivery of curcumin: A suitable approach to decrease cancer stemness markers and regulate miRNAs expression in HT29 colorectal cancer cells

Pakizehkar, S., Ranji, N., Sohi, A. N., & Sadeghizadeh, M. (2019) · Polymers for Advanced Technologies · doi:10.1002/pat.4759 · Cited by 22

Modeling the Efficacy of Oncolytic Adenoviruses In Vitro and In Vivo: Current and Future Perspectives

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Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems

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Emerging Neuroblastoma 3D In Vitro Models for Pre-Clinical Assessments

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Fucoxanthin Holds Potential to Become a Drug Adjuvant in Breast Cancer Treatment: Evidence from 2D and 3D Cell Cultures

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3D Cell Spheroids as a Tool for Evaluating the Effectiveness of Carbon Nanotubes as a Drug Delivery and Photothermal Therapy Agents

Anisimov, R. A., Gorin, D. A., & Abalymov, A. A. (2022) · C – Journal of Carbon Research · doi:10.3390/c8040056 · Cited by 23

Effect of GelMA Hydrogel Properties on Long-Term Encapsulation and Myogenic Differentiation of C2C12 Spheroids

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The 3C (Cell Culture, Computer Simulation, Clinical Trial) Solution for Optimizing the 3R (Replace, Reduction, Refine) Framework during Preclinical Research Involving Laboratory Animals

Roy, S., Parveen, M., Bala, A., & Sur, D. (2025) · ACS Pharmacology & Translational Science · doi:10.1021/acsptsci.4c00661 · Cited by 22

Bioelectronic Interfaces and Sensors for Neural Organoids

Wang, Q., Dong, X., Jiang, D., Tian, S., Qiu, Y. L., Zhu, Y., Wu, J., Shang, S., Zhang, Y., Wang, P., et al. (2025) · Microsystems & Nanoengineering · doi:10.1038/s41378-025-01038-7 · Cited by 21

NIR-responsive nanoplatform for pre/intraoperative image-guided carcinoma surgery and photothermal ablation of residual tumor tissue

Song, J., Zhang, L., Yi, H., Huang, J., Zhang, N., Zhong, Y., Hao, L., Yang, K., Wang, Z., Wang, D., et al. (2019) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2019.102020 · Cited by 21

The interplay of signaling pathway in endothelial cells—matrix stiffness dependency with targeted-therapeutic drugs

Vania, V., Wang, L., Tjakra, M., Zhang, T., Qiu, J., Tan, Y., & Wang, G. (2019) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2019.165645 · Cited by 21

A 3D approach to reproduction

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Three-dimensional cell culture (3DCC) improves secretion of signaling molecules of mesenchymal stem cells (MSCs)

Al-Shaibani, M. B. H. (2022) · Biotechnology Letters · doi:10.1007/s10529-021-03216-9 · Cited by 22

Neural Organoids, a Versatile Model for Neuroscience

Lee, J., & Sun, W. (2022) · Molecules and Cells · doi:10.14348/molcells.2022.2019 · Cited by 22

Physiological Cell Culture Media Tune Mitochondrial Bioenergetics and Drug Sensitivity in Cancer Cell Models

Torres‐Quesada, O., Doerrier, C., Strich, S. L., Gnaiger, E., & Stefan, E. (2022) · Cancers · doi:10.3390/cancers14163917 · Cited by 21

3D Multicellular Tumor Spheroids in a Microfluidic Droplet System for Investigation of Drug Resistance

Lee, S. I., Choi, Y. Y., Kang, S. G., Kim, T. H., Choi, J. W., Kim, Y. J., Kim, T. P., Kang, T., & Chung, B. G. (2022) · Polymers · doi:10.3390/polym14183752 · Cited by 21

Mechanism-Driven and Clinically Focused Development of Botanical Foods as Multitarget Anticancer Medicine: Collective Perspectives and Insights from Preclinical Studies, IND Applications and Early-Phase Clinical Trials

Wang, X., Chan, Y. S., Wong, K., Yoshitake, R., Sadava, D., Synold, T. W., Frankel, P. H., Twardowski, P. W., Lau, C., & Chen, S. (2023) · Cancers · doi:10.3390/cancers15030701 · Cited by 21

Cyclic Stretching Triggers Cell Orientation and Extracellular Matrix Remodeling in a Periodontal Ligament 3D In Vitro Model

Chiu, K., Karpat, M., Hahn, J., Chang, K., Weber, M. H., Wolf, M. F., Aveic, S., & Fischer, H. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202301422 · Cited by 21

Biophysical interplay between extracellular matrix remodeling and hypoxia signaling in regulating cancer metastasis

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GelMA hydrogel: A game-changer in 3D tumor modeling

Jiao, W., Shan, J., Gong, X., Sun, Y., Sang, L., Ding, X., Zhou, H., & Yu, M. (2024) · Materials Today Chemistry · doi:10.1016/j.mtchem.2024.102111 · Cited by 21

A Comparison between Shear Bond Strength of VMK Master Porcelain with Three Base-metal Alloys (Ni-cr-T3, VeraBond, Super Cast) and One Noble Alloy (X-33) in Metal-ceramic Restorations.

Ganjibakhsh, M., Aminishakib, P., Farzaneh, P., Karimi, A., Fazeli, S. A. S., Rajabi, M., Nasimian, A., Naini, F. B., Rahmati, H., Gohari, N. S., et al. (2013) · Europe PMC (PubMed Central) · Cited by 21

The Role of C-X-C Chemokine Receptor Type 4 (CXCR4) in Cell Adherence and Spheroid Formation of Human Ewing’s Sarcoma Cells under Simulated Microgravity

Romswinkel, A., Infanger, M., Dietz, C., Strube, F., & Kraus, A. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20236073 · Cited by 21

Three-Dimensional Cell Culture at the Frontiers of in Vitro Cancer Research: Present Perspectives

Soo, L. L., Zainal, A. A., Hidetsugu, T., Takabatake, K., Nakano, K., Chai, W. L., Nagatsuka, H., & Siar, C. H. (2016) · Annals of Dentistry · doi:10.22452/adum.vol23no1.1

Why 3D in vitro cancer models are the future of cancer research?

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Life is Three Dimensional—As In Vitro Cancer Cultures Should Be

Levinger, I., Ventura, Y., & Vago, R. (2014) · Advances in cancer research · doi:10.1016/b978-0-12-800249-0.00009-3 · Cited by 45

Current Perspectives on 2D and 3D Cell Culture Models in Cancer Research: Molecular Determinants of Tumor Biology and Therapeutic Response

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2D and 3D cell culture: Getting close to mimicking the tumor microenvironment in vitro

Jain, S., Raza, K., Agrawal, A. K., & Vaidya, A. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-817846-1.00027-8