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Cited by 186Neurobiology and Insect Physiology ResearchSleep and Wakefulness ResearchMemory and Neural Mechanisms

A single pair of neurons links sleep to memory consolidation in Drosophila melanogaster

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Haynes, Paula R, Christmann, Bethany L, Griffith, Leslie C·2015·eLife
DOI 10.7554/elife.03868PMC PMC4305081
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Abstract

Sleep promotes memory consolidation in humans and many other species, but the physiological and anatomical relationships between sleep and memory remain unclear. Here, we show the dorsal paired medial (DPM) neurons, which are required for memory consolidation in Drosophila, are sleep-promoting inhibitory neurons. DPMs increase sleep via release of GABA onto wake-promoting mushroom body (MB) α'/β' neurons. Functional imaging demonstrates that DPM activation evokes robust increases in chloride in MB neurons, but is unable to cause detectable increases in calcium or cAMP. Downregulation of α'/β' GABAA and GABABR3 receptors results in sleep loss, suggesting these receptors are the sleep-relevant targets of DPM-mediated inhibition. Regulation of sleep by neurons necessary for consolidation suggests that these brain processes may be functionally interrelated via their shared anatomy. These findings have important implications for the mechanistic relationship between sleep and memory consolidation, arguing for a significant role of inhibitory neurotransmission in regulating these processes.

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  • Co-transmission of a fast-acting transmitter and a neuropeptide was more common.

    “Co-transmission of a fast-acting transmitter and a neuropeptide was more common, e.g., Kenyon cells (at least acetylcholine, sparkly and short neuropeptide F),21,25,55,164 the dorsal paired medial (DPM) neuron (GABA, serotonin, sparkly, nplp1, proctolin, orcokinin, eclosion hormone, CG34136)25,56,60 and most central complex, mushroom body output and mushroom body dopaminergic neurons.25,157”

    From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [56]

    The Results section, “DPMs contain 5HT and GABA, but not ACh or dopamine” (page unavailable), reports that DPM neurons contain GABA and serotonin and suggests they release both. It does not identify the listed neuropeptides in DPM neurons or establish that co-transmission of a fast-acting transmitter and a neuropeptide was more common. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

  • DPM has been found to express the fast-acting transmitter GABA, the monoamine serotonin and several neuropeptides (sparkly, nplp1, proctolin, orcokinin, eclosion hormone, CG34136).

    “DPM has been found to express the fast-acting transmitter GABA the monoamine serotonin and several neuropeptides (sparkly, nplp1, proctolin, orcokinin, eclosion hormone, CG34136).25,56,60”

    From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [56]

    The Results section, “DPMs contain 5HT and GABA, but not ACh or dopamine” (page not provided), reports that DPM neurons contain GABA and 5HT (serotonin). The passages do not establish expression of the six listed neuropeptides; searches for their names and for DPM neuropeptide expression found no supporting passage. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Authors
Haynes, P. R., Christmann, B. L., & Griffith, L. C.
Affiliations
University of California, San Francisco (US)
Corresponding author
Griffith, Leslie C
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2015-01-07
Volume
4
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.03868
PMC
PMC4305081
OpenAlex
W2157991741
Cited by
186
References
8
Citations by year
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FWCI
8.71
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institute of Mental Health (R01 MH067284)
  • National Institute of Neurological Disorders and Stroke (F32 NS045713)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords
sleep, memory consolidation, mushroom body, GABAergic inhibition, GABAA receptor, inhibitory neurotransmission, sleep-promoting neurons, Drosophila melanogaster
MeSH terms
TRPA1 Cation Channel, Acetylcholine, Animals, Dopamine, Drosophila melanogaster, gamma-Aminobutyric Acid, Ion Channels, Memory, Motor Activity, Neural Inhibition, Neurons, Serotonin, Sleep, Synapses, Wakefulness, Mushroom Bodies, Drosophila Proteins, TRPC Cation Channels

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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