Cited by 98Single-cell and spatial transcriptomicsMemory and Neural MechanismsNeuroscience and Neuropharmacology Research

The subiculum is a patchwork of discrete subregions

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Cembrowski, Mark S, Wang, Lihua, Lemire, Andrew L, Copeland, Monique, DiLisio, Salvatore F, Clements, Jody, Spruston, Nelson·2018·eLife

Abstract

In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here, we investigated the extent and organizational principles governing pyramidal cell heterogeneity throughout the mouse subiculum. Using single-cell RNA-seq, we find that the subiculum pyramidal cell population can be deconstructed into eight separable subclasses. These subclasses were mapped onto abutting spatial domains, ultimately producing a complex laminar and columnar organization with heterogeneity across classical dorsal-ventral, proximal-distal, and superficial-deep axes. We further show that these transcriptomically defined subclasses correspond to differential protein products and can be associated with specific projection targets. This work deconstructs the complex landscape of subiculum pyramidal cells into spatially segregated subclasses that may be observed, controlled, and interpreted in future experiments.

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  • Comparing this taxonomy with six previous studies, they found generally good but variable correspondences.

    “Comparing this taxonomy with six previous studies (Cembrowski et al., 2018; Harris et al., 2018; Saunders et al., 2018; Tasic et al., 2018; Yao et al., 2020; Zeisel et al., 2018), we found generally good but variable correspondences”

    From A taxonomy of transcriptomic cell types across the isocortex and hippocampal formation (2021) · cites this paper as Cembrowski et al., 2018

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  1. Paxinos G Franklin KBJ 2004 The Mouse Brain in Stereotaxic Coordinates, Compact Second edition Amsterdam; Boston Elsevier Academic Press

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Authors
Cembrowski, M. S., Wang, L., Lemire, A. L., Copeland, M., DiLisio, S. F., Clements, J., & Spruston, N.
Affiliations
Howard Hughes Medical Institute (US)
Corresponding author
Cembrowski, Mark S
Venue
eLife
Published
2018-10-30
Volume
7
ISSN
2050-084X (ISSN-L)
OpenAlex
W2899509757
Cited by
98
References
46
Citations by year
2026: 10 · 2025: 19 · 2024: 10 · 2023: 9 · 2022: 12 · 2021: 13 · 2020: 14 · 2019: 9 · 2018: 2
FWCI
3.38
Type
journal article
Language
en
Open access
Locations
  • eLife (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Howard Hughes Medical Institute
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
subiculum, pyramidal neurons, neuronal heterogeneity, hippocampal output, laminar organization, columnar organization, dorsal-ventral axis, single-cell transcriptomics, mice
MeSH terms
Animals, Gene Expression Regulation, Hippocampus, Male, Mice, Transgenic, S100 Proteins, Reproducibility of Results, Sequence Analysis, RNA, Pyramidal Cells, Gene Expression Profiling, CA1 Region, Hippocampal, Single-Cell Analysis, Transcriptome

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