Reconstruction of 1,000 projection neurons reveals new cell types and organization of long-range connectivity in the mouse brain
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Abstract
Neuronal cell types are the nodes of neural circuits that determine the flow of information within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides an essential descriptor of cell type and reveals how individual neurons route their output across the brain. Despite the importance of morphology, few projection neurons in the mouse brain have been reconstructed in their entirety. Here we present a robust and efficient platform for imaging and reconstructing complete neuronal morphologies, including axonal arbors that span substantial portions of the brain. We used this platform to reconstruct more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus. Together, the reconstructed neurons constitute more than 85 meters of axonal length and are available in a searchable online database. Axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity.
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Brain-wide complete reconstruction of single neuron morphology is currently the only method to capture the full extent of a neuron's axon projection pattern and define projection neuron types.
“Brain-wide complete reconstruction of single neuron morphology is currently the only approach to capture the full extent of a neuron’s axon projection pattern and define projection neuron types (Gao et al., 2022; Peng et al., 2021; Winnubst et al., 2019).”
From What is a cell type and how to define it? (2022) · cites this paper as Winnubst et al., 2019
Recent technological advances provide resolution and throughput to analyse whole-brain neuronal morphology and comprehensive projection mapping.
“Other recent technological advances provide the resolution and throughput to analyse whole-brain neuronal morphology and comprehensive projection mapping18,19.”
From A multimodal cell census and atlas of the mammalian primary motor cortex (2021) · cites this paper as [18]
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- Authors
- Winnubst, J., Bas, E., Ferreira, T. A., Wu, Z., Economo, M. N., Edson, P., Arthur, B. J., Bruns, C., Rokicki, K., Schauder, D., Olbris, D. J., Murphy, S. D., Ackerman, D. G., Arshadi, C., Baldwin, P., Blake, R., Elsayed, A., Hasan, M., Ramirez, D., Dos Santos, B., Weldon, M., Zafar, A., Dudman, J. T., Gerfen, C. R., Hantman, A. W., Korff, W., Sternson, S. M., Spruston, N., Svoboda, K., & Chandrashekar, J.
- Affiliations
- Howard Hughes Medical Institute (US)
- Rockefeller University (US)
- National Institute of Mental Health (US)
- Corresponding author
- Chandrashekar, Jayaram
- ORCID
- Winnubst, Johan
- Bas, Erhan
- Ferreira, Tiago A.
- Wu, Zhuhao
- Economo, Michael N.
- Rokicki, Konrad
- Olbris, Donald J.
- Ackerman, David G.
- Arshadi, Cameron
- Elsayed, Ahmad
- Hasan, Mashtura
- Dudman, Joshua T.
- Gerfen, Charles R.
- Hantman, Adam W.
- Korff, Wyatt
- Sternson, Scott M.
- Spruston, Nelson
- Svoboda, Karel
- Chandrashekar, Jayaram
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2019-09
- Volume
- 179
- Issue
- 1
- Pages
- 268-281.e13
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC6754285
- OpenAlex
- W2971899185
- Cited by
- 538
- References
- 94
- Citations by year
- 2026: 61 · 2025: 76 · 2024: 73 · 2023: 91 · 2022: 72 · 2021: 88 · 2020: 63 · 2019: 13 · 2014: 1
- FWCI
- 28.52
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- SSRN Electronic Journal (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- OpenBU (Boston University) (submittedVersion)
- Funders
- National Institute of Mental Health
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- projection neurons, neuronal morphology, neuronal cell types, long-range connectivity, mouse brain, motor cortex, thalamus, subiculum, hypothalamus
- MeSH terms
- Animals, Brain, Female, Mice, Inbred C57BL, Mice, Transgenic, Pyramidal Tracts, Software, Transfection, Neurites, Microscopy, Fluorescence, Multiphoton, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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