Anatomical and single-cell transcriptional profiling of the murine habenular complex
Open accessAbstract
The lateral habenula (LHb) is an epithalamic brain structure critical for processing and adapting to negative action outcomes. However, despite the importance of LHb to behavior and the clear anatomical and molecular diversity of LHb neurons, the neuron types of the habenula remain unknown. Here, we use high-throughput single-cell transcriptional profiling, monosynaptic retrograde tracing, and multiplexed FISH to characterize the cells of the mouse habenula. We find five subtypes of neurons in the medial habenula (MHb) that are organized into anatomical subregions. In the LHb, we describe four neuronal subtypes and show that they differentially target dopaminergic and GABAergic cells in the ventral tegmental area (VTA). These data provide a valuable resource for future study of habenular function and dysfunction and demonstrate neuronal subtype specificity in the LHb-VTA circuit.
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Single-cell sequencing and ISH confirmed that these medial habenula neurons reside in the ventral and ventrolateral subregion and form a genetically distinct subtype.
“Single-cell sequencing and in-situ hybridization (ISH) studies confirmed that these neurons reside in the ventral and ventrolateral subregion of the mHb and form a genet-ically distinct subtype.58,59”
From Synaptic and circuit functions of multitransmitter neurons in the mammalian brain (2023) · cites this paper as [58]
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- Authors
- Wallace, M. L., Huang, K. W., Hochbaum, D., Hyun, M., Radeljic, G., & Sabatini, B. L.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Wallace, Michael L
- Sabatini, Bernardo L
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2020-02-11
- Volume
- 9
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC7012610
- OpenAlex
- W3006252764
- Cited by
- 113
- References
- 66
- Citations by year
- 2026: 13 · 2025: 15 · 2024: 30 · 2023: 15 · 2022: 16 · 2021: 14 · 2020: 10
- FWCI
- 6.30
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Howard Hughes Medical Institute
- National Institute of Neurological Disorders and Stroke (NS103226, NS105883)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- lateral habenula, medial habenula, neuronal cell types, ventral tegmental area, dopaminergic neurons, GABAergic neurons, single-cell transcriptomics, mice
- MeSH terms
- Animals, Brain Mapping, Ventral Tegmental Area, Habenula, Gene Expression Profiling, Mice, Single-Cell Analysis, Dopaminergic Neurons, GABAergic Neurons, Transcriptome
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