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Cited by 11Cellular transport and secretionFungal and yeast genetics researchAutophagy in Disease and Therapy

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The VINE complex is an endosomal VPS9-domain GEF and SNX-BAR coat

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Shortill, Shawn P, Frier, Mia S, Wongsangaroonsri, Ponthakorn, Davey, Michael, Conibear, Elizabeth·2022·eLife
DOI 10.7554/elife.77035PMC PMC9507130

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Abstract

Membrane trafficking pathways perform important roles in establishing and maintaining the endosomal network. Retrograde protein sorting from the endosome is promoted by conserved SNX-BAR-containing coat complexes including retromer which enrich cargo at tubular microdomains and generate transport carriers. In metazoans, retromer cooperates with VARP, a conserved VPS9-domain GEF, to direct an endosomal recycling pathway. The function of the yeast VARP homolog Vrl1 has been overlooked due to an inactivating mutation found in commonly studied strains. Here, we demonstrate that Vrl1 has features of a SNX-BAR coat protein and forms an obligate complex with Vin1, the paralog of the retromer SNX-BAR protein Vps5. Unique features in the Vin1 N-terminus allow Vrl1 to distinguish it from Vps5, thereby forming a complex that we have named VINE. The VINE complex occupies endosomal tubules and redistributes a conserved mannose 6-phosphate receptor-like protein from endosomes. We also find that membrane recruitment by Vin1 is essential for Vrl1 GEF activity, suggesting that VINE is a multifunctional coat complex that regulates trafficking and signaling events at the endosome.

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Metadata

Authors
Shortill, S. P., Frier, M. S., Wongsangaroonsri, P., Davey, M., & Conibear, E.
Affiliations
University of British Columbia (CA)
Corresponding author
Conibear, Elizabeth
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2022-08-08
Volume
11
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.77035
PMC
PMC9507130
OpenAlex
W4290648813
Cited by
11
References
3
Citations by year
2026: 3 · 2025: 5 · 2024: 2 · 2022: 1
FWCI
1.07
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Natural Sciences and Engineering Research Council of Canada (2022-04573, PGS-D Doctoral Scholarship, CGS-M Frederick Banting and Charles Best Canada Graduate Scholarship)
  • Canadian Institutes of Health Research (247169, 365914)
  • Canada Foundation for Innovation (Leading Edge Fund 30636)
  • BC Children's Hospital Research Institute (Jan M Friedman Graduate Studentship)
  • University of British Columbia (Catalyst Paper Corporation Affiliated Fellowship, 4 Year Doctoral Fellowship, Medical Genetics Rotation Award)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
Keywords
retromer, endosomal recycling, mannose 6-phosphate receptor, membrane trafficking, yeast
MeSH terms
Mannose, Phosphates, Endosomes, Saccharomyces cerevisiae, Guanine Nucleotide Exchange Factors, Protein Transport, Saccharomyces cerevisiae Proteins, Vesicular Transport Proteins, Sorting Nexins

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