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Cited by 15Neuroscience and Neural EngineeringElectrochemical sensors and biosensorsTissue Engineering and Regenerative Medicine

Intravital microscopy evaluation of angiogenesis and its effects on glucose sensor performance

Open access
Koschwanez, H. E., Reichert, W. M., Klitzman, B.·2010·Journal of Biomedical Materials Research Part A
DOI 10.1002/jbm.a.32630PMC PMC2916729
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Abstract

An optical window model for the rodent dorsum was used to perform chronic and quantitative intravital microscopy and laser Doppler flowmetry of microvascular networks adjacent to functional and non‐functional glucose sensors. The one‐sided configuration afforded direct, real‐time observation of the tissue response to bare (unmodified, smooth surface) sensors and sensors coated with porous poly‐L‐lactic acid (PLLA). Microvessel length density and red blood cell flux (blood perfusion) within 1 mm of the sensors were measured bi‐weekly over 2 weeks. When non‐functional sensors were fully implanted beneath the windows, the porous coated sensors had two‐fold more vasculature and significantly higher blood perfusion than bare sensors on Day 14. When functional sensors were implanted percutaneously, as in clinical use, no differences in baseline current, neovascularization, or tissue perfusion were observed between bare and porous coated sensors. However, percutaneously implanted bare sensors had two‐fold more vascularity than fully implanted bare sensors by Day 14, indicating the other factors, such as micromotion, might be stimulating angiogenesis. Despite increased angiogenesis adjacent to percutaneous sensors, modest sensor current attenuation occurred over 14 days, suggesting that factors other than angiogenesis may play a dominant role in determining sensor function. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010

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  • Despite increased vasculature, sensor sensitivity did not increase.

    “Despite such increases in vasculature, the sensitivity of the sensor did not increase”

    From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [109]

  • Angiogenesis is not the only factor in assessing the biocompatibility of CGM sensors.

    “indicating that angiogenesis is not the only factor in assessing the biocompatibility of CGM sensors.109”

    From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [109]

  • Vessel length and perfusion of the vasculature increased during the 14-day implantation period.

    “The vessel length and perfusion of the vasculature increased during the implantation period (i.e., 14 d).”

    From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [109]

  • At 10 and 14 days after implantation, vessel lengths and blood perfusion of porous materials were significantly greater than bare sensor coatings when implanted subcutaneously.

    “At 10 and 14 d post-implantation, the vessel lengths and blood perfusion of the porous materials was significantly greater than bare (i.e., non-porous) sensor coatings when implanted subcutaneously.”

    From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [109]

  • The same materials implanted percutaneously produced no observable differences.

    “Despite these observations, the same materials implanted percutaneously resulted in no observable differences.”

    From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [109]

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Research works printed but not linked: 3

  1. [9]Makale MT Lin JT Calou RE Tsai AG Chen PC Gough DA Tissue window chamber system for validation of implanted oxygen sensors American Journal of Physiology-Heart and Circulatory Physiology 2003 Jun 284 6 H2288 H2294 12598234
  2. [29]Von Recum AF New aspects of biocompatibility: motion at the interface Heimke G Soltesz U Lee A Clinical implant materials, advances in biomaterials Amsterdam Elsevier Science Publishers BV 1990 297 302
  3. [36]Dungel P Long N Yu B Moussy Y Moussy F Study of the effects of tissue reactions on the function of implanted glucose sensors Journal of Biomedical Materials Research Part A 2008 Jun 85A 3 699 706 17876777

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Authors
Koschwanez, H. E., Reichert, W. M., & Klitzman, B.
Affiliations
Duke University (US)
Corresponding author
Reichert, W. M.
ORCID
Venue
Journal of Biomedical Materials Research Part A
Publisher
Wiley
Published
2009-11-12
Volume
93A
Issue
4
Pages
1348-1357
ISSN
  • 1549-3296 (ISSN-L)
  • 1552-4965
DOI
10.1002/jbm.a.32630
PMC
PMC2916729
OpenAlex
W1984396335
Cited by
15
References
40
Citations by year
2022: 2 · 2021: 1 · 2019: 1 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 1
FWCI
0.69
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Journal of Biomedical Materials Research Part A (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • DukeSpace (Duke University) (submittedVersion, cc-by-nc-nd)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases (DK54932, R01DK054932, R56DK054932)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
glucose sensor, angiogenesis, intravital microscopy, laser Doppler flowmetry, microvascular networks, blood perfusion, percutaneous implantation, neovascularization, micromotion, implantable biosensors
MeSH terms
Animals, Blood Glucose, Erythrocytes, Glucose, Male, Microscopy, Neovascularization, Pathologic, Perfusion, Polyesters, Polymers, Time Factors, Biosensing Techniques, Porosity, Rats, Sprague-Dawley, Lactic Acid, Rats, Microvessels

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