Cited by 74Antifungal resistance and susceptibilityFungal and yeast genetics researchBacterial biofilms and quorum sensing

Proteomics of drug resistance in Candida glabrata biofilms

Seneviratne, C. Jayampath, Wang, Yu, Jin, Lijian, Abiko, Y., Samaranayake, Lakshman P.·2010·PROTEOMICS

Abstract

Candida glabrata is a fungal pathogen that causes a variety of mucosal and systemic infections among compromised patient populations with higher mortality rates. Previous studies have shown that biofilm mode of the growth of the fungus is highly resistant to antifungal agents compared with the free-floating or planktonic mode of growth. Therefore, in the present study, we used 2-D DIGE to evaluate the differential proteomic profiles of C. glabrata under planktonic and biofilm modes of growth. Candida glabrata biofilms were developed on polystyrene surfaces and age-matched planktonic cultures were obtained in parallel. Initially, biofilm architecture, viability, and antifungal susceptibility were evaluated. Differentially expressed proteins more than 1.5-fold in DIGE analysis were subjected to MS/MS. The transcriptomic regulation of these biomarkers was evaluated by quantitative real-time PCR. Candida glabrata biofilms were highly resistant to the antifungals and biocides compared with the planktonic mode of growth. Candida glabrata biofilm proteome when compared with its planktonic proteome showed upregulation of stress response proteins, while glycolysis enzymes were downregulated. Similar trend could be observed at transcriptomic level. In conclusion, C. glabrata biofilms possess higher amount of stress response proteins, which may potentially contribute to the higher antifungal resistance seen in C. glabrata biofilms.

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Crossref · 10.1002/pmic.200900611

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  • Upregulation of Trx1p as a stress-response protein exerts defences to C. glabrata against oxidative stress.

    “Furthermore, the upregulation of Trx1p as a stress-response protein exerts defences to C. glabrata against oxidative stress [72].”
  • Stressed C. glabrata biofilms show a positive regulatory protein response associated with the stress response, including heat shock and other stress proteins Hsp12, Trx1, and Pep4.

    “Seneviratne et al. [163] showed that there is a positive regulatory protein response associated with the stress response in biofilms of stressed C. glabrata, as displayed by the heat shock and other stress proteins (Hsp12, Trx1, and Pep4).”

    From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [163]

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Authors
Seneviratne, C. J., Wang, Y., Jin, L., Abiko, Y., & Samaranayake, L. P.
Affiliations
  • University of Hong Kong (HK)
  • Nihon University (JP)
Corresponding author
Samaranayake, Lakshman P.
Venue
PROTEOMICS
Publisher
Wiley
Published
2010-04
Volume
10
Issue
7
Pages
1444-1454
ISSN
  • 1615-9853 (ISSN-L)
  • 1615-9861
OpenAlex
W2038113654
Cited by
74
References
51
Citations by year
2025: 2 · 2024: 5 · 2023: 2 · 2022: 4 · 2021: 7 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 6 · 2016: 2 · 2015: 7 · 2014: 4 · 2013: 7 · 2012: 10
FWCI
5.18
Type
journal article
Language
en
Open access
closed
Locations
  • PROTEOMICS (publishedVersion)
  • PubMed (publishedVersion)
  • The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
Funders
Hong Kong Research Grants Council (HKU 7624/06M)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords
Candida glabrata, biofilm, antifungal resistance, planktonic versus biofilm growth, proteomic profiling, stress proteins, biocide resistance, fungal virulence factors, differentially expressed proteins
MeSH terms
Stress, Physiological, Electrophoresis, Gel, Two-Dimensional, Polymerase Chain Reaction, Biofilms, Gene Expression Profiling, Drug Resistance, Fungal, Proteomics, Candida glabrata

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