Intestinal microflora stimulates myoelectric activity of rat small intestine by promoting cyclic initiation and aboral propagation of migrating myoelectric complex
Abstract
Microbial modulation of myoelectric activity in small intestine was studied. Germ-free male Sprague-Dawley rats were equipped with bipolar electrodes from the duodenojejunal junction to the midpoint of small intestine. Prior to and one week after introduction of conventional intestinal microflora, 32 +/- 5% and 61 +/- 5% (mean +/- SE), respectively, of activity fronts of the migrating myoelectric complex reached the midpoint (P < 0.05), and the interval between activity fronts in proximal jejunum was reduced from 31.2 +/- 2.0 min to 17.5 +/- 0.8 min, respectively (P < 0.01). The pattern of propagation was more regular after conventionalization. Slow-wave frequency in proximal jejunum was 38.5 +/- 1.2/min in germ-free rats and 43.0 +/- 0.8/min in conventional rats (P < 0.01), but introduction of microflora failed to increase the frequency in germ-free rats. The frequency of spike potentials succeeding jejunal infusion of 5 ml of 12.5% glucose remained unchanged after conventionalization. Statistical analyses showed that the interval between activity fronts varied mainly within rats, whereas the propagation velocity showed statistically significant variability between rats (P < 0.01), regardless of intestinal microflora. Luminal control by the resident microflora is important for physiological cycling and aboral propagation of the migrating myoelectric complex, but seems to be of no major consequence for postprandial myoelectric response.
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From Gut microbiota role in irritable bowel syndrome: New therapeutic strategies (2016) · cites this paper as [95]
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- Authors
- Husebye, E., Hellström, P. M., & Midtvedt, T.
- Affiliations
- Karolinska University Hospital (SE)
- Venue
- Digestive Diseases and Sciences
- Published
- 1994-05
- Volume
- 39
- Issue
- 5
- Pages
- 946-956
- ISSN
- 0163-2116 (ISSN-L)
- 1573-2568
- OpenAlex
- W2068183409
- Cited by
- 169
- References
- 26
- Citations by year
- 2025: 2 · 2024: 5 · 2023: 3 · 2022: 7 · 2021: 9 · 2019: 1 · 2018: 7 · 2017: 8 · 2016: 12 · 2015: 15 · 2014: 7 · 2013: 7 · 2012: 7
- FWCI
- 4.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Digestive Diseases and Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
- Keywords
- gut microbiota, migrating motor complex, small intestine motility, germ-free rats, slow wave frequency, glucose infusion, gut-brain axis
- MeSH terms
- Action Potentials, Animals, Bacteria, Eating, Gastrointestinal Transit, Germ-Free Life, Intestine, Small, Male, Myoelectric Complex, Migrating, Rats, Sprague-Dawley, Rats
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