Enzyme-digested fucoidan extracts derived from seaweed Mozuku of Cladosiphon novae-caledoniae kylin inhibit invasion and angiogenesis of tumor cells
Open accessAbstract
Fucoidan is a uniquely-structured sulfated polysaccharide found in the cell walls of several types of brown seaweed that has recently, especially as enzyme-digested fucoidan extract, attracted a lot attention due to its anti-tumor potential. In this study, we evaluated the effects of enzyme-digested fucoidan extracts prepared from seaweed Mozuku of Cladosiphon novae-caledoniae kylin on in vitro invasion and angiogenesis abilities of human tumor cells. First, we evaluated the effect of the fucoidan extracts on oxidative stress of tumor cells, and demonstrated that intracellular H(2)O(2) level and released H(2)O(2) from tumor cells were both greatly repressed upon the treatment with the fucoidan extracts, suggesting that fucoidan extracts ameliorate oxidative stress of tumor cells. Next, we tested for the effects of fucoidan extracts on invasion ability of human fibrosarcoma HT1080 cells, showing that fucoidan extracts significantly inhibit their invasion, possibly via suppressing matrix metalloproteinases (MMPs) MMP-2/9 activities. Further, we investigated the effects of the fucoidan extracts on angiogenesis of human uterine carcinoma HeLa cells, and found that fucoidan extracts suppressed expression and secretion of an angiogenesis factor vascular endothelial growth factor (VEGF), resulting in suppressed vascular tubules formation of tumor cells. The results taken together clarified that enzyme-digested fucoidan extracts from Cladosiphon novae-caledoniae kylin possess inhibitory effects on invasion and angiogenesis of tumor cells. These effects might, at least partially, be elicited by the antioxidative potential of enzyme digested fucoidan extracts.
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Fucoidan from C. novae-caledonia kylin digested with abalone glycosidase reduced MMP-2/9 activities and decreased VEGF expression, with subsequent inhibition of invasion and suppression of tubule formation in tumor cells.
“Fucoidan from C. novae-caledonia kylin digested with the abalone glycosidase was responsible for the reduction of MMP-2/9 activities and the decrease in VEGF expression with subsequent inhibition of invasion and suppression of tubules formation in tumor cells [54].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [54]
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Cites
Production of large amounts of hydrogen peroxide by human tumor cells.
Szatrowski, T. P., & Nathan, C. (1991) · PubMed · Cited by 2,504 · see citing works
Regulation of matrix metalloproteinase expression in tumor invasion
Westermarck, J. K., & Kähäri, V. (1999) · The FASEB Journal · doi:10.1096/fasebj.13.8.781 · Cited by 1,494 · see citing works
Vascular Endothelial Growth Factor
Ferrara, N. (2009) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.108.179663 · Cited by 1,283 · see citing works
Rupérez, P., Ahrazem, O., & Leal, J. A. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf010908o · Cited by 599 · see citing works
Oversulfation of fucoidan enhances its anti-angiogenic and antitumor activities
Koyanagi, S., Tanigawa, N., Nakagawa, H., Soeda, S., & Shimeno, H. (2003) · Biochemical Pharmacology · doi:10.1016/s0006-2952(02)01478-8 · Cited by 479 · see citing works
Berkman, R. A., Merrill, M. J., Reinhold, W. C., Monacci, W. T., Saxena, A., Clark, W. C., Robertson, J. T., Ali, I. U., & Oldfield, E. H. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116165 · Cited by 462 · see citing works
Grote, K., Flach, I., Luchtefeld, M., Akin, E., Holland, S. M., Drexler, H., & Schieffer, B. (2003) · Circulation Research · doi:10.1161/01.res.0000077044.60138.7c · Cited by 417 · see citing works
Cancer invasion and tissue remodeling: common themes in proteolytic matrix degradation
Johnsen, M., Lund, L. R., Rømer, J., et al. (1998) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(98)80044-6 · Cited by 360 · see citing works
Wizigmann-Voos, S., Breier, G., Risau, W., & Plate, K. H. (1995) · PubMed · Cited by 326 · see citing works
Liabakk, N., Talbot, I. C., Smith, R. A., Wilkinson, K., & Balkwill, F. R. (1996) · PubMed · Cited by 322 · see citing works
Mattern, J., Koomägi, R., & Volm, M. (1996) · British Journal of Cancer · doi:10.1038/bjc.1996.166 · Cited by 311 · see citing works
Ruch, W., Cooper, P. H., & Baggiolini, M. (1983) · Journal of Immunological Methods · doi:10.1016/s0022-1759(83)80008-8 · Cited by 277 · see citing works
Guidi, A. J., Abu‐Jawdeh, G. M., Berse, B., Jackman, R. W., Tognazzi, K., Dvorak, H. F., & Brown, L. F. (1995) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/87.16.1237 · Cited by 276 · see citing works
Johnson, L. L., Dyer, R. D., & HUPE, D. J. (1998) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(98)80122-1 · Cited by 270 · see citing works
Zhang, Q., Li, N., Zhou, G., Lu, X., Xu, Z., & Li, Z. (2003) · Pharmacological Research · doi:10.1016/s1043-6618(03)00103-8 · Cited by 253 · see citing works
Masuda, M., Suzui, M., Lim, J. T. E., Deguchi, A., Soh, J., & Weinstein, I. B. (2002) · Journal of Experimental Therapeutics and Oncology · doi:10.1046/j.1359-4117.2002.01062.x · Cited by 235 · see citing works
Upregulation of vascular endothelial growth factor by H2O2 in rat heart endothelial cells
Chua, C. C., Hamdy, R. C., & Chua, B. H. L. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00115-4 · Cited by 220 · see citing works
Suzuki, K., Hayashi, N., Miyamoto, Y., et al. (1996) · PubMed · Cited by 216 · see citing works
Xue, C., Yu, G., Hirata, T., Terao, J., & Lin, H. (1998) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.62.206 · Cited by 197 · see citing works
Hydrogen peroxide stimulates macrophage vascular endothelial growth factor release
Cho, M., Hunt, T. K., & Hussain, M. (2001) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.2001.280.5.h2357 · Cited by 197 · see citing works
Vascular endothelial growth factor
Ferrara, N. (1993) · Trends in Cardiovascular Medicine · doi:10.1016/1050-1738(93)90046-9 · Cited by 188 · see citing works
Antitumor Active Fucoidan from the Brown Seaweed,<i>Umitoranoo</i>(<i>Sargassum thunbergii</i>)
Zhuang, C., Itoh, H., Mizuno, T., & Ito, H. (1995) · Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.59.563 · Cited by 184 · see citing works
Antioxidant activities of sulfated polysaccharide fractions from Porphyra haitanesis
Zhang, Q., Yu, P., Li, Z., Zhang, H., Xu, Z., & Li, P. (2003) · Journal of Applied Phycology · doi:10.1023/a:1025137728525 · Cited by 173 · see citing works
(2003) · PubMed · Cited by 170 · see citing works
Brauchle, M. A., Funk, J. O., Kind, P., & Werner, S. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.36.21793 · Cited by 158 · see citing works
Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica
Xue, C., Fang, Y., Lin, H., Chen, L., Li, Z., Deng, D., & Lu, C.-X. (2001) · Journal of Applied Phycology · doi:10.1023/a:1008103611522 · Cited by 147 · see citing works
Okuzumi, J., Nishino, H., Murakoshi, M., Iwashima, A., Tanaka, Y., Yamane, T., Fujita, Y., & Takahashi, T. (1990) · Cancer Letters · doi:10.1016/0304-3835(90)90068-9 · Cited by 142 · see citing works
Belkhiri, A., Richards, C. D., Whaley, M., McQueen, S. A., & Orr, F. (1997) · PubMed · Cited by 140 · see citing works
Zhao, X., Xue, C., Li, Z., Cai, Y.-P., Liu, H., & Qi, H. (2004) · Journal of Applied Phycology · doi:10.1023/b:japh.0000044822.10744.59 · Cited by 116 · see citing works
Okuzumi, J., Takahashi, T., Yamane, T., Kitao, Y., Inagake, M., Ohya, K., Nishino, H., & Tanaka, Y. Y. (1993) · Cancer Letters · doi:10.1016/0304-3835(93)90142-v · Cited by 113 · see citing works
Antitumor activity of marine algae
Noda, H., Amano, H., Arashima, K., et al. (1990) · Hydrobiologia · doi:10.1007/bf00040290 · Cited by 109 · see citing works
Albini, A., Morini, M., D’Agostini, F., Ferrari, N., Campelli, F., Arena, G., Noonan, D. M., Pesce, C. M., & Flora, S. D. (2001) · PubMed · Cited by 95 · see citing works
Tang, F., & Meydani, M. (2001) · Nutrition and Cancer · doi:10.1080/01635581.2001.9680622 · Cited by 90 · see citing works
Role of reactive oxygen species in IL-1β-stimulated sustained ERK activation and MMP-9 induction
Gurjar, M. V., Deleon, J., Sharma, R. V., & Bhalla, R. C. (2001) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.2001.281.6.h2568 · Cited by 90 · see citing works
Antitumor activity of marine algae
Noda, H., Amano, H., Arashima, K., et al. (1990) · Springer eBooks · doi:10.1007/978-94-009-2049-1_84 · Cited by 86 · see citing works
Qian, Y. R., Luo, J., Leonard, S. S., Harris, G. K., Millecchia, L., Flynn, D. C., & Shi, X. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m207517200 · Cited by 83 · see citing works
Chabut, D., Fischer, A., Colliec‐Jouault, S., Laurendeau, I., Matou, S., Bonniec, B. L., & Helley, D. (2003) · Molecular Pharmacology · doi:10.1124/mol.64.3.696 · Cited by 81 · see citing works
Inhibition of Angiogenesis and Promotion of Melanoma Dormancy by Vitamin E Succinate
Malafa, M. P., Fokum, F. D., Smith, L., & Louis, A. (2002) · Annals of Surgical Oncology · doi:10.1007/bf02574523 · Cited by 73 · see citing works
Kolev, K., Skopál, J., Simon, L., et al. (2003) · Thrombosis and Haemostasis · doi:10.1160/th03-02-0070 · Cited by 64 · see citing works
Agarwal, A., Muñoz-Nájar, U., Klueh, U., et al. (2004) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)63727-3 · Cited by 64 · see citing works
Usui, T., Asari, K., & Mizuno, T. (1980) · Agricultural and Biological Chemistry · doi:10.1271/bbb1961.44.1965 · Cited by 63 · see citing works
Cancer chemoprevention by natural carotenoids and their related compounds
Nishino, H. (1995) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240590829 · Cited by 62 · see citing works
Usui, T., Asari, K., & Mizuno, T. (1980) · Agricultural and Biological Chemistry · doi:10.1080/00021369.1980.10864249 · Cited by 48 · see citing works
Yamamoto, I., Takahashi, M., Suzuki, T., Seino, H., & Mori, H. (1984) · PubMed · Cited by 46 · see citing works
Balsari, A., Maier, J. A. M., Colnaghi, M. I., et al. (1999) · PubMed · Cited by 42 · see citing works
Soeda, S., Ishida, S., Shimeno, H., & Nagamatsu, A. (1994) · Japanese Journal of Cancer Research · doi:10.1111/j.1349-7006.1994.tb02920.x · Cited by 39 · see citing works
Upregulation of vascular endothelial growth factor by hydrogen peroxide in human colon cancer
Zhu, J.-W. (2002) · World Journal of Gastroenterology · doi:10.3748/wjg.v8.i1.153 · Cited by 34 · see citing works
Chabut, D., Fischer, A.-M., Helley, D., et al. (2004) · Thrombosis Research · doi:10.1016/j.thromres.2004.01.013 · Cited by 33 · see citing works
In vitro effects of fucans on MDA-MB231 tumor cell adhesion and invasion.
Haroun-Bouhedja, F., Lindenmeyer, F., Lu, H., Soria, C., Jozefonvicz, J., & Boisson‐Vidal, C. (2002) · PubMed · Cited by 30 · see citing works
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Brown seaweed fucoidan: Biological activity and apoptosis, growth signaling mechanism in cancer
Senthilkumar, K., Manivasagan, P., Venkatesan, J., & Kim, S.-K. (2013) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2013.06.030 · Cited by 340 · see citing works
Fucoidan present in brown algae induces apoptosis of human colon cancer cells
Kim, E. J., Park, S. Y., Lee, J.-Y., & Park, J. H. Y. (2010) · BMC Gastroenterology · doi:10.1186/1471-230x-10-96 · Cited by 275 · see citing works
Microalgae in modern cancer therapy: current knowledge
Abd El-Hack, M. E., Abdelnour, S., Alagawany, M., Abdo, M., Sakr, M. A., Khafaga, A. F., Mahgoub, S. A., Elnesr, S. S., & Gebriel, M. G. (2019) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2018.12.069 · Cited by 206 · see citing works
Anticancer properties and mechanisms of fucoidan on mouse breast cancer in vitro and in vivo
Xue, M., Ge, Y., Zhang, J., Wang, Q., Hou, L., Liu, Y., Sun, L., & Li, Q. (2012) · PLoS ONE · doi:10.1371/journal.pone.0043483 · Cited by 192 · see citing works
Lee, H., Kim, J.-S., & Kim, E. (2012) · PLoS ONE · doi:10.1371/journal.pone.0050624 · Cited by 191 · see citing works
Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
Fedorov, S., Ermakova, S., Zvyagintseva, T., & Stonik, V. (2013) · Marine Drugs · doi:10.3390/md11124876 · Cited by 189 · see citing works
Holtkamp, A. D., Kelly, S., Ulber, R., & Lang, S. (2009) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1790-x · Cited by 175 · see citing works
Zhang, Z., Teruya, K., Eto, H., & Shirahata, S. (2011) · PLoS ONE · doi:10.1371/journal.pone.0027441 · Cited by 167 · see citing works
Self-citationAntioxidant and antiproliferative activities of heterofucans from the seaweed Sargassum filipendula
Costa, L. S., Fidelis, G. P., Telles, C. B. S., Dantas-Santos, N., Camara, R. B. G., Cordeiro, S. L., Costa, M. S. S. P., Almeida-Lima, J., Melo-Silveira, R. F., Oliveira, R. M., et al. (2011) · Marine Drugs · doi:10.3390/md9060952 · Cited by 155 · see citing works
Tsai, H.-L., Tai, C.-J., Huang, C.-W., Chang, F.-R., & Wang, J.-Y. (2017) · Marine Drugs · doi:10.3390/md15040122 · Cited by 156 · see citing works
Magalhaes, K. D., Costa, L. S., Fidelis, G. P., Oliveira, R. M., Nobre, L. T. D. B., Dantas-Santos, N., Camara, R. B. G., Albuquerque, I. R. L., Cordeiro, S. L., Sabry, D. A., et al. (2011) · International Journal of Molecular Sciences · doi:10.3390/ijms12053352 · Cited by 97 · see citing works
Athukorala, Y., Ahn, G. N., Jee, Y.-H., Kim, G.-Y., Kim, S.-H., Ha, J.-H., Kang, J.-S., Lee, K.-W., & Jeon, Y.-J. (2009) · Journal of Applied Phycology · doi:10.1007/s10811-008-9368-7 · Cited by 89 · see citing works
Vishchuk, O., Ermakova, S., & Zvyagintseva, T. (2013) · Marine Drugs · doi:10.3390/md11010194 · Cited by 50 · see citing works
Applications of Marine Nutraceuticals in Dairy Products
Vidanarachchi, J. K., Kurukulasuriya, M. S., Malshani Samaraweera, A., & Silva, K. F. S. T. (2012) · Advances in food and nutrition research · doi:10.1016/b978-0-12-416003-3.00030-5 · Cited by 24 · see citing works
- Authors
- Ye, J., Li, Y., Teruya, K., Katakura, Y., Ichikawa, A., Eto, H., Hosoi, M., Hosoi, M., Nishimoto, S., & Shirahata, S.
- Affiliations
- Kyushu University (JP)
- Kyoto Institute of Technology (JP)
- Daiichi-Sankyo (Japan) (JP)
- Corresponding author
- Shirahata, Sanetaka
- Venue
- Cytotechnology
- Published
- 2005-01
- Volume
- 47
- Issue
- 1-3
- Pages
- 117-126
- ISSN
- 0920-9069 (ISSN-L)
- 1573-0778
- PMC
- PMC3449827
- OpenAlex
- W2132589866
- Cited by
- 146
- References
- 50
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 2 · 2023: 14 · 2022: 9 · 2021: 3 · 2020: 7 · 2019: 7 · 2018: 10 · 2017: 7 · 2016: 6 · 2015: 5 · 2014: 21 · 2013: 14 · 2012: 9
- FWCI
- 2.02
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cytotechnology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
- Keywords
- fucoidan, enzymatic digestion, sulfated polysaccharides, tumor invasion, angiogenesis, oxidative stress, hydrogen peroxide, matrix metalloproteinases, MMP-2, MMP-9, fibrosarcoma, HeLa cells, vascular endothelial growth factor, antioxidant activity, anticancer activity
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