Anticancer properties and mechanisms of fucoidan on mouse breast cancer in vitro and in vivo
Open accessAbstract
BACKGROUND: Fucoidan is a sulfated polysaccharide derived from brown algae that has been reported to perform multiple biological activities, including antitumor activity. In this study, we examined the influence of crude fucoidan on mouse breast cancer in vitro and in vivo. MATERIALS AND METHODS: In vitro, fluorescent staining, flow cytometry and Western blot were performed to analyze apoptosis and vascular endothelial growth factor (VEGF) expression of mouse breast cancer 4T1 cells. In vivo, therapy experiments were conducted on Babl/c mice bearing breast cancer. The tumor volume and weight were measured. The number of apoptotic cells and microvascular density (MVD) in tumor tissues were assessed by TUNEL and CD34 immunostaining. Immunohistochemical assays and ELISA assay were used to detect the expression of VEGF in tissues. RESULTS: In vitro studies showed that crude fucoidan significantly decreased the viable number of 4T1 cells, induced apoptosis and down-regulated the expression of VEGF. The expression of Bcl-2 was decreased, and the ratio of Bcl-2 to Bax was significantly decreased. The expression of Survivin and phosphorylated extracellular signal regulated protein kinases (ERKs) was decreased. Cytochrome C was released from mitochondria into cytosol, and the cleaved Caspase-3 protein rose after fucoidan treatment. Intraperitoneal injection of fucoidan in breast cancer models reduced the tumor volume and weight. The enhanced antitumor efficacy was associated with decreased angiogenesis and increased induction of apoptosis. CONCLUSION: These findings indicated that crude fucoidan inhibited mouse breast cancer growth in vitro and in vivo. These data suggest that fucoidan may serve as a potential therapeutic agent for breast cancer.
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Treatment of mouse breast cancer cells with fucoidan showed enhanced antitumor activity associated with decreased angiogenesis via down-regulation of VEGF and increased induction of apoptosis.
“Treatment of mouse breast cancer cells with fucoidan showed that the enhanced antitumor activity was associated with decreased angiogenesis via the down-regulation of vascular endothelial growth factor and increased induction of apoptosis [65].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [65]
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Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
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- Authors
- Xue, M., Ge, Y., Zhang, J., Wang, Q., Hou, L., Liu, Y., Sun, L., & Li, Q.
- Affiliations
- Qingdao University (CN)
- Affiliated Hospital of Qingdao University (CN)
- Corresponding author
- Ge, Yinlin
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-08-20
- Volume
- 7
- Issue
- 8
- Pages
- e43483
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3423341
- OpenAlex
- W1988249582
- Cited by
- 192
- References
- 35
- Citations by year
- 2026: 6 · 2025: 13 · 2024: 8 · 2023: 13 · 2022: 15 · 2021: 15 · 2020: 13 · 2019: 22 · 2018: 14 · 2017: 19 · 2016: 14 · 2015: 18 · 2014: 15 · 2013: 7
- FWCI
- 18.74
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Keywords
- fucoidan, breast cancer, anticancer activity, apoptosis, angiogenesis, vascular endothelial growth factor, 4T1 cells, Bcl-2/Bax ratio, survivin, ERK1/2 phosphorylation, cytochrome c release, caspase-3, microvessel density, sulfated polysaccharides, brown algae, tumor growth inhibition
- MeSH terms
- Survivin, Animals, Antineoplastic Agents, Breast Neoplasms, Cell Line, Enzyme-Linked Immunosorbent Assay, Female, Fibroblasts, Flow Cytometry, Polysaccharides, Repressor Proteins, Blotting, Western, Apoptosis, Proto-Oncogene Proteins c-bcl-2, Vascular Endothelial Growth Factor A, Inhibitor of Apoptosis Proteins, Mice
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